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Freezability And Fertility Of Thai Native Chicken Semen In Different Diluents, Pachara Thananurak, Napapach Chuaychu-Noo, Thevin Vongpralub
Freezability And Fertility Of Thai Native Chicken Semen In Different Diluents, Pachara Thananurak, Napapach Chuaychu-Noo, Thevin Vongpralub
The Thai Journal of Veterinary Medicine
The objective of the present study was to compare the efficacy of three diluents on post-thaw motility, viability, and fertility of frozen chicken semen. Semen from Thai native cocks (Pradu Hang Dam; n=24) was collected, pooled, and randomly allocated into three groups, then diluted at a ratio of 1:3 (v:v) with Schramm, EK, and polyvinylpyrrolidone (PVP)-based medium. The diluted semen samples were cooled down to 5ºC and then mixed with Dimethylformamide (DMF) (6% v/v). Semen straws were subjected to cryopreservation using liquid nitrogen vapor method. For cryopreserved sperm quality assessment, the frozen semen straws were thawed, and motility was assessed …
Effects Of Cryopreservation And Leukodepletion On Morphological Index Of Red Blood Cells In Dogs, Deniz Aktaran Bala, Ibrahim Akyazi
Effects Of Cryopreservation And Leukodepletion On Morphological Index Of Red Blood Cells In Dogs, Deniz Aktaran Bala, Ibrahim Akyazi
The Thai Journal of Veterinary Medicine
Preservation of blood components for transfusion is a long-standing phenomenon. However, studies of cryopreservation of leukodepleted canine erythrocytes are quite limited and no studies are available with respect to morphological evaluation of erythrocytes during the cryopreservation process. The aim of this study was to determine morphological changes caused by cryopreservation, storage and leukocyte filtration. Healthy adult dogs meeting the criteria for blood transfusion were used in this study. Packed red blood cells (pRBCs) were obtained from each dog. The samples were divided into two groups. Leukocyte filtration was performed on one group of pRBCs. The other group received no filtration. …