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Development Of Indirect Enzyme-Linked Immunosorbent Assay For Antibody Detection Against Avibacterium Paragallinarum, Panchita Hongprasertkul, Wisanu Wanasawaeng, Niwat Chansiripornchai
Development Of Indirect Enzyme-Linked Immunosorbent Assay For Antibody Detection Against Avibacterium Paragallinarum, Panchita Hongprasertkul, Wisanu Wanasawaeng, Niwat Chansiripornchai
The Thai Journal of Veterinary Medicine
Indirect enzyme-linked immunosorbent assay (I-ELISA) for antibody detection against A. paragallinarum serovars A, B and C was developed in separated plates. One hundred Babcock 308 female layer chickens were randomly divided into five groups of 20 each. Groups 1, 2, 3 and 4 were different positive control groups of immunized chickens with commercial trivalent mineral oil vaccine, and prepared bacterins of Avibacterium paragallinarum serovars A (221), B (0222) and C (Modesto), respectively. The chickens in Group 5 were assigned as a negative control. Positive and negative control sera from the chickens in Groups 1-5 at 2 weeks after the second …