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Full-Text Articles in Medicine and Health Sciences
Semen Production Capacity Among Boar Breeds And Effect Of Organic Antioxidant Supplementation On Sperm Characteristics During The Hot Season In Thailand, Congbang Ngo, Suriya Sooksong, Nutthee Am-In, Padet Tummaruk
Semen Production Capacity Among Boar Breeds And Effect Of Organic Antioxidant Supplementation On Sperm Characteristics During The Hot Season In Thailand, Congbang Ngo, Suriya Sooksong, Nutthee Am-In, Padet Tummaruk
The Thai Journal of Veterinary Medicine
The present study was carried out to investigate semen production capacity among boar breeds and measure the efficiency of a new organic antioxidant supplementation on semen production and qualities during the hot season in Thailand. A total of 711 ejaculates from 87 boars (i.e., 26 Duroc, 15 Landrace, 30 Yorkshire and 16 crossbred Landrace × Yorkshire) were included in the study. Semen samples were allocated into two treatments – without organic antioxidant (CONTROL; n = 397) and with organic antioxidant supplementation (TREATMENT; n = 314). The experiment was carried out for three months during the hot season in May (n …
Comparison Of The Effects Of Different Thawing Methods On Post-Thaw Sperm Characteristics Of Buffalo Bull Semen, Eser Akal, Murat Selçuk, Burcu Esi̇n, Melih Akar, Cumali Kaya
Comparison Of The Effects Of Different Thawing Methods On Post-Thaw Sperm Characteristics Of Buffalo Bull Semen, Eser Akal, Murat Selçuk, Burcu Esi̇n, Melih Akar, Cumali Kaya
The Thai Journal of Veterinary Medicine
Application proper procedures for thawing of cryopreserved sperm are required for successful artificial insemination. The study was aimed to compare the effects of watery and dry thawing systems on motility, acrosome integrity, plasma membrane integrity, morphologic and kinematic characteristics of buffalo bull spermatozoa. Anatolian Buffalo bull spermatozoa, cryopreserved in volumes of 0.25 mL straws, were thawed by using 1) watery thawing system; a total of 10 straws were thawed in a water bath at 37 °C for 30 sec and 2) dry thawing system; a total of 10 straws were thawed in a dry thawing device at 37 °C for …
Boar Sperm Production In A Tropical Environment, Janyaporn Rungruangsak, Somchai Sangkaphet, Kakanang Buranaamnuay, Panupan Pongpeng, Padet Tummaruk
Boar Sperm Production In A Tropical Environment, Janyaporn Rungruangsak, Somchai Sangkaphet, Kakanang Buranaamnuay, Panupan Pongpeng, Padet Tummaruk
The Thai Journal of Veterinary Medicine
In a tropical environment, sow productivity is strongly associated with boar sperm production. Regular monitoring of semen quality in boars that produce semen for artificial insemination is important. The objective of the present study was to investigate the boar sperm production in an evaporative cooling system in a tropical environment in association with breed and season. A total of 2878 ejaculates from 68 boars were collected from a boar stud in the western region of Thailand. The breeds consisted of 22 Duroc, 22 Yorkshire, 18 Landrace and six hybrid boars. Seasons were classified as cool, hot or rainy. Semen volume, …
Cryopreservation Of Boar Semen: Where We Are, Panida Chanapiwat, Kampon Kaeoket
Cryopreservation Of Boar Semen: Where We Are, Panida Chanapiwat, Kampon Kaeoket
The Thai Journal of Veterinary Medicine
The preservation of boar semen can be divided intotwo methods as follows: fresh boar semen and frozen boar semen. The first method is only to preserve the sperm for a short period, ranging from 3 to 10 days and depends on the semen extender used, however, its benefit is only in use for artificial insemination on a particular farm or for a short distant transportation. The latter method has been developed in order to keep the semen of superior genetic boars for long distant transportation or preservation of their genetics for future production or to set upa new herd in …
Effects Of Centrifugation And Removal Of Seminal Plasma On Motility Of Fresh Boar Sperm, Olivia Bury, Victoria Mcrae, Jose Len, Kate Plush, Roy N. Kirkwood
Effects Of Centrifugation And Removal Of Seminal Plasma On Motility Of Fresh Boar Sperm, Olivia Bury, Victoria Mcrae, Jose Len, Kate Plush, Roy N. Kirkwood
The Thai Journal of Veterinary Medicine
Extended semen from 6 boars was centrifuged and then gently resuspended (CR), had the supernatant decanted to remove residual seminal plasma and resuspended in new extender (CD), or served as non-treated controls. Measures of sperm motility were assessed immediately after centrifugation and then every 24 h until 96 h using a computer-assisted sperm analyser. Total and progressive sperm motilities were reduced (P<0.05) by 48 h in CD. Total motility was not affected in CR sperm but progressive motility was reduced (P<0.05) at 72 h. Changes with time were very similar for VSL, VAP, VCL and BCF. Control values remained relatively stable but VCL, VSL, VAP and ALH were lower within minutes of centrifugation and decanting of CD sperm. Both CR and CD treatment values for all variables were lower (P<0.05) from 3 d. These data indicate that centrifugation can negatively affect boar sperm motility and that even the small amount of residual seminal plasma in fresh-extended boar semen has a protective role. Sperm age-associated reduced motility can be countered with caffeine.
Effects Of Environmental Factors, Ages And Breeds On Semen Characteristics In Thai Indigenous Chickens: A One-Year Study, Pornjit Sonseeda, Thevin Vongpralub, Banyat Laopaiboon
Effects Of Environmental Factors, Ages And Breeds On Semen Characteristics In Thai Indigenous Chickens: A One-Year Study, Pornjit Sonseeda, Thevin Vongpralub, Banyat Laopaiboon
The Thai Journal of Veterinary Medicine
Several reports on the semen characteristics of domestic fowl have indicated that environmental factors, breed and age significantly affect semen quality. There is, however, a paucity of data on effects of environmental factor, breed and age on semen characteristic in Thai indigenous chickens. The aims of the present study were to investigate the effects of annual ambient temperature and humidity, local breeds (i.e. Lueng hang kaow, Pradoo hang dam and Chee), and ages (10 and 18 months) on sperm production and semen quality among Thai native cocks. Thirty-six Thai native cocks were housed under natural environmental conditions, and semen was …
A Comparative Study On The Efficacy Of Four Semen Extenders And Thawing By Seminal Plasma On The Quality Of Frozenthawed Boar Semen, Chonthida Rienprayoon, Chonlapat Klangnak, Suttiporn Onton, Chanyuth Tretipskul, Padet Tummaruk
A Comparative Study On The Efficacy Of Four Semen Extenders And Thawing By Seminal Plasma On The Quality Of Frozenthawed Boar Semen, Chonthida Rienprayoon, Chonlapat Klangnak, Suttiporn Onton, Chanyuth Tretipskul, Padet Tummaruk
The Thai Journal of Veterinary Medicine
The present study investigated the influence of four different commercial semen extenders used during theholding time and the use of seminal plasma as a thawing medium on the frozen-thawed (FT) boar semen quality.Sperm-rich fractions were collected from 11 boars and were divided into four groups according to the type of semenextender used during the holding time (A, B, C and D). The extended semen was cooled down and centrifuged. Thesperm pellets were diluted in lactose-egg yolk extender plus glycerol and Equex STM Paste. The semen were loadedinto 0.5 ml straws and frozen. Thawing was achieved by immersing the straws in …
The Sperm Dna Damage After Cryopreservation Of Boar Semen In Relation To Post-Thawed Semen Qualities, Antioxidant Supplementation And Boars Effects, Panida Chanapiwat, Kampon Kaeoket, Padet Tummaruk
The Sperm Dna Damage After Cryopreservation Of Boar Semen In Relation To Post-Thawed Semen Qualities, Antioxidant Supplementation And Boars Effects, Panida Chanapiwat, Kampon Kaeoket, Padet Tummaruk
The Thai Journal of Veterinary Medicine
The objectives of the present study were to evaluate the damage of DNA of the frozen-thawed (FT) boar spermatozoa and to investigate the effect of various concentrations of L-cysteine supplementation on the sperm DNA damage. A total of 104 cryopreserved semen samples from twenty-six ejaculates of 16 proven boars were analyzed. Of these samples, each semen sample contained a different concentration of L-cysteine i.e., 0 (n=41), 5 (n=41), 10 (n=11) and 15 (n=11) mM. All of the semen samples were cryopreserved by controlled-rate freezer. The semen was thawed at 50oC for 12 sec and the damage to the sperm DNA …
The Effect Of Cold Storage On The Quality Of Stallion Semen And Pregnancy Rate After Artificial Insemination, Theerawat Tharasanit, Sukanya Manee-In, Pharaphon Khamenkhetwit, Sudson Sirivaidyapong, Chainarong Lohachit
The Effect Of Cold Storage On The Quality Of Stallion Semen And Pregnancy Rate After Artificial Insemination, Theerawat Tharasanit, Sukanya Manee-In, Pharaphon Khamenkhetwit, Sudson Sirivaidyapong, Chainarong Lohachit
The Thai Journal of Veterinary Medicine
While the use of frozen-thawed semen has been hampered by poor cryopreservability of equine sperm, the technique of choice in equine practice remains artificial insemination (AI) using cold storage semen. This study examined the effect of cold storage on semen quality and pregnancy rate after AI. In experiment I, ejaculates were collected from 3 stallions of proven fertility and then diluted to a final concentration of 25-50 x 106 sperm/ml. After transportation, the semen was then divided into two aliquots. The first part of semen was centrifuged to partially remove the seminal plasma, while the remaining was left intact with …
The Epididymal Sperm Survival Interval Following Orchidectomy In Dogs, Sudson Sirivaidyapong, Theerawat Swangchan-Uthai
The Epididymal Sperm Survival Interval Following Orchidectomy In Dogs, Sudson Sirivaidyapong, Theerawat Swangchan-Uthai
The Thai Journal of Veterinary Medicine
The aim of the study was to investigate the motility and viability of canine epididymal spermatozoa, collected from the epididymides following orchidectomy (castration), over a period of time. Sperm samples were collected at 25-28oC from the caudal epididymis, 0, 1, 3, 6, and 12 hours, after the castration of 75 fertile dogs (1-10 years old). Progressive motility and viability of the epididymal sperm decreased over a period of time, after the collection. The epididymal sperm motility was 82.3±6, 76.3±8, 72.0±7, 60.3±9, and 22.7±20% while the percentage of viable spermatozoa was 85.5±5, 78.8±7, 77.5±7, 64.4±7, and 27.1±21 at 0, 1, 3, …
Effects Of Sperm Preparation On In Vitro Fertilization In Pig, Mongkol Techakumphu, Wichai Tantasuparak
Effects Of Sperm Preparation On In Vitro Fertilization In Pig, Mongkol Techakumphu, Wichai Tantasuparak
The Thai Journal of Veterinary Medicine
The aim of these experiments was to investigate the effects of sperm preparation on the success rate of in vitro fertilization in the pig. Three factors, the time period for capacitation, the type of sperm and the sperm motility-stimulating factors were studied by investigating their effect on the cleavage of pig follicular oocytes, after IVM-IVF-IVC. A total of 1,235 immature oocytes, collected from 3 to 6 mm follicles of gilt ovaries gathered at slaughterhouse were allocated to 3 experiments. Immature oocytes were cultivated in TCM199 NaHCO3 supplemented with 10 μg/ml of FSH/LH and 1 μg/ml of Estradiol-17ẞ for 44 hrs. …