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The Thai Journal of Veterinary Medicine

Journal

2022

Isolation

Articles 1 - 2 of 2

Full-Text Articles in Medicine and Health Sciences

Unravelling The Productivity Rate Of Selective Media For Brucella Strains, Mustafa Sencer Karagul, Serkan Ikiz Dec 2022

Unravelling The Productivity Rate Of Selective Media For Brucella Strains, Mustafa Sencer Karagul, Serkan Ikiz

The Thai Journal of Veterinary Medicine

The aim of this study is to reveal the productivity rate of selective media for Brucella strains. Brucella selective media namely Farrell’s medium, CITA, Modified Thayer-Martin, Jones-Morgan medium and one additional medium including erythritol and antibiotics were compared within the scope of productivity rate and colony growth in time. The comparison was carried out using 14 strains including Brucella abortus biovars, S19 vaccine strain, Brucella suis biovars and Brucella ovis. Erythritol addition showed a positive effect on colony growth in time. In addition to differences between the productivity rates of the media; there was also some variation in the colony …


Molecular Detection And Isolation Of Mycoplasma Conjunctivae From Goats With Infectious Keratoconjunctivitis In Sichuan Province, Southwest China, Youwen Yang, Yanmin Li, Xiaofang Dao, Lei Fei, Falong Yang Sep 2022

Molecular Detection And Isolation Of Mycoplasma Conjunctivae From Goats With Infectious Keratoconjunctivitis In Sichuan Province, Southwest China, Youwen Yang, Yanmin Li, Xiaofang Dao, Lei Fei, Falong Yang

The Thai Journal of Veterinary Medicine

Infectious keratoconjunctivitis (IKC) is a common contagious ocular disease in ruminants worldwide characterized with keratitis and conjunctivitis and, in severe cases, blindness. Recently two outbreaks of infectious keratoconjunctivitis (IKC) occurred in goats in Sichuan Province, China. To identify the causative agent responsible for these outbreaks, ocular swabs were then taken from 40 goats with IKC and 22 goats without IKC for isolation and molecular detection of mycoplasma. Mycoplasma conjunctivae-specific nested-PCR was 85.0% positive among samples from goats with IKC and 40.9% positive from goats without IKC. Nine PCR products were sequenced and showed that there was 99.6-100% sequence homology among …