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Seroprevalence And Molecular Detection Of Lawsonia Intracellularis From Asymptomatic Horses In Korea, Yeonsu Oh, Md Mukter Hossain, Ho-Seong Cho
Seroprevalence And Molecular Detection Of Lawsonia Intracellularis From Asymptomatic Horses In Korea, Yeonsu Oh, Md Mukter Hossain, Ho-Seong Cho
The Thai Journal of Veterinary Medicine
The study was performed to investigate the prevalence pattern of Lawsonia intracellularis in horses in Korea via serology and molecular detection. Total of 138 horse sera and 112 fecal samples, in pairs with sera, were collected from five provinces across the country. The sera were analyzed using blocking enzyme-linked immunosorbent assay (bELISA) to detect L. intracellularis-specific antibodies and the fecal samples were evaluated by the real-time PCR assay to detect DNA of L. intracellularis. Results showed that 94% of the horses had antibodies and the younger the age, the higher the seropositivity; “<2 years” (92%), “2-5 years” (96%), “6-10 years” (94%), and “>10 years” (93%). DNA of L. intracellularis …