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Astrocytes Derived From P53-Deficient Mice Provide A Multistep In Vitro Model For Development Of Malignant Gliomas., Alan M. Yahanda Md, Facs, J M Bruner, L A Donehower, R S Morrison
Astrocytes Derived From P53-Deficient Mice Provide A Multistep In Vitro Model For Development Of Malignant Gliomas., Alan M. Yahanda Md, Facs, J M Bruner, L A Donehower, R S Morrison
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Loss or mutation of p53 is thought to be an early event in the malignant transformation of many human astrocytic tumors. To better understand the role of p53 in their growth and transformation, we developed a model employing cultured neonatal astrocytes derived from mice deficient in one (p53 +/-) or both (p53 -/-) p53 alleles, comparing them with wild-type (p53 +/+) cells. Studies of in vitro and in vivo growth and transformation were performed, and flow cytometry and karyotyping were used to correlate changes in growth with genomic instability. Early-passage (EP) p53 -/- astrocytes achieved higher saturation densities and had …