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Full-Text Articles in Medicine and Health Sciences
Targeting Ho-1 By Epigallocatechin-3-Gallate Reduces Contrast-Induced Renal Injury Via Anti-Oxidative Stress And Anti-Inflammation Pathways., Zhao Gao, Yu Han, Yunhui Hu, Xiaoyan Wu, Yongbin Wang, Xiaoqun Zhang, Jinjuan Fu, Xue Zou, Jun Zhang, Xiongwen Chen, Pedro A. Jose, Xi Lu, Chunyu Zeng
Targeting Ho-1 By Epigallocatechin-3-Gallate Reduces Contrast-Induced Renal Injury Via Anti-Oxidative Stress And Anti-Inflammation Pathways., Zhao Gao, Yu Han, Yunhui Hu, Xiaoyan Wu, Yongbin Wang, Xiaoqun Zhang, Jinjuan Fu, Xue Zou, Jun Zhang, Xiongwen Chen, Pedro A. Jose, Xi Lu, Chunyu Zeng
Medicine Faculty Publications
Both oxidative stress and inflammation are involved in the pathogenesis of contrast-induced nephropathy (CIN). Epigallocatechin-3-gallate (EGCG), a purified catechin from green tea, has antioxidant and anti-inflammatory effects. However, it is unknown whether or not EGCG is effective in treating CIN. Our present study found that intravenous administration of EGCG, either before or just after the establishment of CIN, had a protective effect, determined by normalization of serum creatinine and blood urea nitrogen levels, improvement in renal histopathological scoring and alleviation of apoptosis, accompanied by decreased oxidative stress and inflammation. Because EGCG is a potent inducer of the antioxidant heme oxygenase-1 …
Genetic Modification Of Human Mesenchymal Stem Cells Helps To Reduce Adiposity And Improve Glucose Tolerance In An Obese Diabetic Mouse Model., Sabyasachi Sen, Cleyton C Domingues, Carol Rouphael, Cyril Chou, Chul Kim, Nagendra Yadava
Genetic Modification Of Human Mesenchymal Stem Cells Helps To Reduce Adiposity And Improve Glucose Tolerance In An Obese Diabetic Mouse Model., Sabyasachi Sen, Cleyton C Domingues, Carol Rouphael, Cyril Chou, Chul Kim, Nagendra Yadava
Medicine Faculty Publications
INTRODUCTION: Human mesenchymal stem cells (MSCs) are multipotent cells that can differentiate into fat, muscle, bone and cartilage cells. Exposure of subcutaneous abdominal adipose tissue derived AD-MSCs to high glucose (HG) leads to superoxide accumulation and up-regulation of inflammatory molecules. Our aim was to inquire how HG exposure affects MSCs differentiation and whether the mechanism is reversible.
METHODS: We exposed human adipose tissue derived MSCs to HG (25 mM) and compared it to normal glucose (NG, 5.5 mM) exposed cells at 7, 10 and 14 days. We examined mitochondrial superoxide accumulation (Mitosox-Red), cellular oxygen consumption rate (OCR, Seahorse) and gene …