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The Role Of Connective Tissue Growth Factor In Maintaining The Epithelial Phenotype Of Ovarian Cancer Cells During Epithelial-To-Mesenchymal Transition, Harry Ramos, Sandra Galoforo, Gil Mor, Radhika Gogoi
The Role Of Connective Tissue Growth Factor In Maintaining The Epithelial Phenotype Of Ovarian Cancer Cells During Epithelial-To-Mesenchymal Transition, Harry Ramos, Sandra Galoforo, Gil Mor, Radhika Gogoi
Medical Student Research Symposium
Objective: The transition from epithelial to mesenchymal is essential for the process of ovarian cancer (OC) metastasis. The aim of our study is to evaluate the role and mechanism of Connective Tissue Growth Factor (CTGF) in epithelial to mesenchymal transition (EMT).
Methods: R182 is an epithelial OC cell line. CTGF expression +/- Transforming Growth Factor -b (TGF-b) was determined via Western blot and ELISA. R182 CTGF knock out (KO) were derived utilizing a Cas9/CRISPR-Cas9 lentivirus plasmid vector. Anoikis resistance and invasion assays were performed to characterized phenotypes of R182 wild type (WT) and KO cells. For anoikis resistance, cells …