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Intratumor Heterogeneity In Epithelial Cancer: Assessment In Cell Line Models And Circulating Tumor Cells, Wimba W. Dinutanayo, Ingeborg T. Keilholz, Diana Braunholz
Intratumor Heterogeneity In Epithelial Cancer: Assessment In Cell Line Models And Circulating Tumor Cells, Wimba W. Dinutanayo, Ingeborg T. Keilholz, Diana Braunholz
Makara Journal of Health Research
Background: CTCs are present only in small numbers in patients' blood. This study aimed to establish a protocol for enumeration and phenotypic characterization of circulating tumor cells (CTCs) by ImageStreamX MK II (AMNIS) imaging flow cytometry and to characterize the expression of epithelial, mesenchymal, and stem cell markers in CTCs. Methods: The study used the FaDu cell line at different passages, cisplatin-resistant (FaDu CDDP-R), and irradiation-resistant (FaDu IR-R) subclones, as well as blood samples from head and neck squamous cell carcinoma (HNSCC) and colorectal cancer (CRC) patients for CTC detection (n = 5). Cells were fixed using 4% paraformaldehyde and …