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An Investigation On The Interaction Of Various Natural Products With The Amyloid-Β Peptide; Betulinic Acid Causes Rapid Amyloid-Β Fibril Formation At The Expense Of Soluble Oligomers, Matthew Planchard
Honors Theses
In this report we investigate the interaction of the natural compounds asiatic acid (AA), asiaticoside (AS), umbelliferone (UM), and betulinic acid (BA) with the amyloid-β (Aβ) peptide, the aggregation of which is implicated to be the major pathogenic event in Alzheimer's Disease (AD). Screening of compounds for ability to affect Aβ aggregation was performed via ThT fluorescence. Only BA showed consistent deviation from controls, causing virtually instantaneous formation of large amounts of fibrils in a concentration-dependent fashion. Turbidity assays ensured the aggregation monitored via ThT was not experimental aberration. Kinetics utilizing ThT and turbidity indicated that BA causes immediate exponential …