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Full-Text Articles in Medicine and Health Sciences

Stress-Adaptation And Stress-Induced Changes In Campylobacter Jejuni, Geetha Sanal Kumar-Phillips Dec 2012

Stress-Adaptation And Stress-Induced Changes In Campylobacter Jejuni, Geetha Sanal Kumar-Phillips

Graduate Theses and Dissertations

The foodborne pathogen Campylobacter jejuni is one of the leading causes of human gastroenteritis. This bacterium is not a robust organism compared with many other foodborne pathogens and requires special conditions in the laboratory for its growth. In nature, however, this organism is able to survive in very diverse and hostile environments and produce disease in humans. The different mechanisms by which C. jejuni survives stressful conditions in the environment remain unclear. Adhesion and invasion are thought to be important factors for the colonization of C. jejuni in the intestinal tracts of hosts. Previous research in our laboratory showed that …


Genetic Analysis Of Chromosomal Regions Affecting Sperm Mobility In The Chicken (Gallus Gallus Domesticus), Sharath Chandra Munnaluri May 2012

Genetic Analysis Of Chromosomal Regions Affecting Sperm Mobility In The Chicken (Gallus Gallus Domesticus), Sharath Chandra Munnaluri

Graduate Theses and Dissertations

This project is focused on determining the contributions of particular genetic loci to low sperm mobility in the chicken. Sperm mobility has been shown to be a major determinant of male fertility in broiler breeders. A whole genome SNP scan identified chromosomal regions (QTLs) that control sperm mobility. I used microsatellites from 4 chromosomal regions ( 15.151 on chromosome 6, 12.435 on chromosome Z, 12.341 on chromosome Z, and 17.214 on chromosome Z) to determine the association of these regions with sperm mobility in experimental lines divergently selected for mobility phenotype. Determination of the genetics underlying sperm mobility will identify …


Lameness Associated With A Possible Neural Problem In The Lower Spinal Cord Of Chickens, Gallus Gallus, Gurueswar Nagarajan May 2012

Lameness Associated With A Possible Neural Problem In The Lower Spinal Cord Of Chickens, Gallus Gallus, Gurueswar Nagarajan

Graduate Theses and Dissertations

Since the second half of the twentieth century lameness in broiler chickens has been known to be caused by different types of disorders, however the etiology of several type of lameness remain unknown. Because of the intense selection of broilers for rapid growth, some birds are more prone to physiological insults resulting in lameness. This study focuses on possible neural problems in the lower spinal cord associated with lameness in broilers. Broilers were raised in pens with wire floors and provided with food and water ad libitum. Three groups of birds 1) birds displaying a normal gait (Controls), 2) lame …


Investigation Of A Locus On Chromosome 9 For Contributions To Pulmonary Hypertension Syndrome In Broilers, Sriram Krishnamoorthy May 2012

Investigation Of A Locus On Chromosome 9 For Contributions To Pulmonary Hypertension Syndrome In Broilers, Sriram Krishnamoorthy

Graduate Theses and Dissertations

Although the ascites syndrome in chickens has been investigated for years, it continues to inflict financial losses to the world poultry industry. It is estimated that 8% of the 361 million broiler deaths are due to ascites leading to losses of millions of dollars annually. Efforts to curb the incidence of ascites are typically designed to slow early growth. This limits the birds' ability to show its true genetic potential and impacts later yields. In 1994 lines divergent for susceptibility to ascites were established from a commercial sire line through sibling selection of birds reared at local altitude after testing …


Feed Supplementation With Natural Extracts Of Cranberry And Its Efficacy On Campylobacter Colonization In Poultry, Ann Woo-Ming May 2012

Feed Supplementation With Natural Extracts Of Cranberry And Its Efficacy On Campylobacter Colonization In Poultry, Ann Woo-Ming

Graduate Theses and Dissertations

Campylobacter spp. has been identified as one of the leading causative agents of food borne diarrheal illness. Epidemiological evidence has shown that poultry is the main source for human infection. Poultry are asymptomatic carriers of Campylobacter within their gastrointestinal tract, with colonization levels reaching 106-108 cfu/g cecal content. Surveys of domestic poultry flocks have estimated that approximately 90% of flocks are positive for Campylobacter colonization. Risk assessment studies have determined that by reducing levels of Campylobacter colonization during rearing, incidences of human infection will be significantly reduced. Currently there are no consistently effective treatments to eliminate Campylobacter from poultry flocks. …


A Role Of Environmental Components In Autoimmune Vitiligo Of Smyth Line Chickens, Lei Dong May 2012

A Role Of Environmental Components In Autoimmune Vitiligo Of Smyth Line Chickens, Lei Dong

Graduate Theses and Dissertations

The Smyth line (SL) of chickens is a well-established animal model for the study of spontaneous autoimmune vitiligo, displaying all the characteristics of human vitiligo. In humans, phenolic derivative 4-tertiary butyl phenol (4-TBP) has been shown to trigger vitiligo in susceptible individuals. In vitiligo-prone SL-chickens, live turkey herpesvirus (HVT) infection at hatch was identified as the most effective trigger of SL-vitiligo expression. This dissertation examined the role of the environmental factors 4-TBP and HVT in the etiology of SL-vitiligo. Specifically, the generation of reactive-oxygen-species by melanocyte-containing feather tissue (MC-FT) and cultured melanocytes in response to 4-TBP exposure in vitro (Study …


Characterization Of The Vasotocin Receptor Subtype 4 (Vt4r) In The Brain And Pituitary Gland Of The Chicken, Gallus Gallus, Rajamani Selvam May 2012

Characterization Of The Vasotocin Receptor Subtype 4 (Vt4r) In The Brain And Pituitary Gland Of The Chicken, Gallus Gallus, Rajamani Selvam

Graduate Theses and Dissertations

The present study investigated the distribution of the vasotocin subtype four receptor (VT4R) in brain and pituitary gland of the chicken, Gallus gallus. The anterior pituitary cell types associated with the VT4R were also determined. Two polyclonal antibodies were raised in rabbit against a cocktail of peptides, 15 amino acids from the amino terminal region and 17 amino acids from the carboxy terminal region of VT4R receptor. The antibody was validated utilizing the Western blot and immunocytochemistry. A single band at 47KDa utilizing membrane protein extracts of chicken brain and pituitary tissues was shown for the VT4R antibody. A peptide …