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Porcine Bladder Urothelial, Myofibroblast And Detrusor Muscle Cells: Characterisation And Atp Release, Ying Cheng, Kylie J. Mansfield, Shaun L. Sandow, Prajni Sadananda, Elizabeth Burcher, Kate H. Moore

Graduate School of Medicine - Papers (Archive)

ATP is released from the bladder mucosa in response to stretch, but the cell types responsible are unclear. Our aim was to isolate and characterize individual populations of urothelial, myofibroblast, and detrusor muscle cells in culture, and to examine agonist-stimulated ATP release. Using female pig bladders, urothelial cells were isolated from bladder mucosa following trypsin-digestion of the luminal surface. The underlying myofibroblast layer was dissected, minced, digested, and cultured until confluent (10–14 days). A similar protocol was used for muscle cells. Cultures were used for immunocytochemical staining and/or ATP release investigations. In urothelial cultures, immunoreactivity was present for the cytokeratin …