Medicine and Health Sciences Commons^™

Extracellular Chaperones Prevent Aβ42-Induced Toxicity In Rat Brains, Roberta Cascella, Simona Conti, Francesca Tatini, Elisa Evangelisti, Tania Scartabelli, Fiorella Casamenti, Mark R. Wilson, Fabrizio Chiti, Cristina Cecchi

Faculty of Science, Medicine and Health - Papers: part A

Alzheimer's disease (AD) is a progressive neurodegenerative disorder characterised by cognitive decline, formation of the extracellular amyloid β (Aβ42) plaques, neuronal and synapse loss, and activated microglia and astrocytes. Extracellular chaperones, which are known to inhibit amyloid fibril formation and promote clearance of misfolded aggregates, have recently been shown to reduce efficiently the toxicity of HypF-N misfolded oligomers to immortalised cell lines, by binding and clustering them into large species. However, the role of extracellular chaperones on Aβ oligomer toxicity remains unclear, with reports often appearing contradictory. In this study we microinjected into the hippocampus of rat brains Aβ42 oligomers …

Genomic Homeostasis Is Dysregulated In Favour Of Apoptosis In The Colonic Epithelium Of The Azoxymethane Treated Rat, Caroline A. Kerr, Barney M. Hines, Janet M. Shaw, Robert Dunne, Lauren M. Bragg, Julie Clarke, Trevor Lockett, Richard Head

Faculty of Science, Medicine and Health - Papers: part A

Background The acute response to genotoxic carcinogens in rats is an important model for researching cancer initiation events. In this report we define the normal rat colonic epithelium by describing transcriptional events along the anterior-posterior axis and then investigate the acute effects of azoxymethane (AOM) on gene expression, with a particular emphasis on pathways associated with the maintenance of genomic integrity in the proximal and distal compartments using whole genome expression microarrays. Results There are large transcriptional changes that occur in epithelial gene expression along the anterior-posterior axis of the normal healthy rat colon. AOM administration superimposes substantial changes on …

The Effects Of Insufflation Conditions On Rat Mesothelium, Andrew K. Davey, Jessica Hayward, Jean K. Marshall, Anthony E. Woods

Faculty of Science, Medicine and Health - Papers: part A

Aim. The aim of this investigation was to examine the alterations in the peritoneum after cold dry CO2, heated dry CO2, and humidified heated CO2 at pressures equivalent to intraperitoneal pressures used in human laparoscopy. Methods. Eighteen rats were divided into 4 treatment groups—group 1: untreated control; group 2: insufflation with cold dry CO2; group 3: insufflation with heated, dry CO2; group 4: insufflation with heated and humidified CO2. The abdomen was insufflated to 5 mm/Hg (flow rate 50 mL/min) for 2 h. Twelve hours later, tissue samples were collected for analysis by light microscopy (LM) and scanning electron microscopy …

Effects Of Dexamethasone On Posterior Capsule Opacification-Like Changes In A Rat Lens Explant Model, Kylie Mansfield, Anna Cerra, Coral Chamberlain

Faculty of Science, Medicine and Health - Papers: part A

Purpose: Many patients whose sight is initially restored by cataract surgery eventually suffer secondary loss of vision because of posterior capsule opacification (PCO; after-cataract), a condition in which lens epithelial cells left behind at surgery become aberrant and migrate into the light path. The aim of this study was to determine whether dexamethasone (DEX), an anti-inflammatory agent widely used before and after cataract surgery, influences the behavior of lens cells under conditions relevant to PCO development.

Methods: An established rat PCO model was used in which explanted epithelial cells attached to the lens capsule are exposed sequentially to TGFβ2 and …

Fgf-2 Counteracts Loss Of Tgfβ Affected Cells From Rat Lens Explants: Implications For Pco (After Cataract), Kylie Mansfield, Anna Cerra, Coral Chamberlain

Faculty of Science, Medicine and Health - Papers: part A

Purpose: While cataract surgery initially benefits most patients, many suffer secondary loss of vision because of posterior capsule opacification (PCO). Lens epithelial cells left behind at surgery become aberrant and migrate into the light path. TGF-beta (TGFβ) appears to play a key role in this process by inducing the cells to undergo an epithelial-mesenchymal transition. Paradoxically, it also typically induces them to undergo apoptotic death. The present study was undertaken to investigate the hypothesis that FGF plays a role in PCO formation by promoting the survival of abnormal cells with PCO-like characteristics.

Methods: Rat lens epithelial explants were cultured for …

The Reduced Effect Of Serotonin On Oxygen Consumption During Muscle Contraction In The Autoperfused Rat Hindlimb, Andrew Hoy, Gregory E. Peoples, Peter L. Mclennan

Faculty of Science, Medicine and Health - Papers: part A

Serotonin (5-HT) has been shown to reduce skeletal muscle oxygen consumption cY 02) during resting conditions in a variety of animal models. It is thought to act through redistribution of blood flow within skeletal muscle directing flow away from muscle tissue (nutritive bed) towards less metabolically active tissue, adipose and septum (non-nutritive bed) by selective vasoconstriction. The aim of this study was to test whether the effects of 5-HT (previously observed under resting conditions) are reproducible during the increased metabolic demand of muscle contraction.