Medicine and Health Sciences Commons^™

Activation Of Ca2+-Activated K+ Channels And Cell Migration By Hepatocyte Growth Factor/Scatter Factor In Madin-Darby Canine Kidney Cells., Min Jin

Electronic Theses and Dissertations

Hepatocyte Growth Factor/Scatter Factor (HGF/SF) stimulates migration of various cells and has been linked via Met tyrosine kinase signaling to transformation and the metastatic phenotype. HGF/SF-Met signaling also plays a role in malignancy. Migration of transformed MDCK-F cells depends on activation of a charybdotoxin (ChTX)-sensitive, volume-activated membrane K⁺ current. Patch-clamp electrophysiology and transwell migration assays were used to study the effects of HGF/SF on membrane K⁺ currents and cell migration in MDCK II cells. HGF/SF activated membrane K⁺ currents that increased over 24 hr, and these could be modulated by altering intracellular free calcium concentration [Ca^{2+ …}