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Articles 1 - 2 of 2
Full-Text Articles in Medicine and Health Sciences
In Vivo Construction Of Recombinant Molecules Within The Caenorhabditis Elegans Germ Line Using Short Regions Of Terminal Homology, Benedict J. Kemp, Julia Hatzold, Laura A. Sternick, Joshua Cornman-Homonoff, Jessica M. Whitaker, Pamela J. Tieu, Eric J. Lambie
In Vivo Construction Of Recombinant Molecules Within The Caenorhabditis Elegans Germ Line Using Short Regions Of Terminal Homology, Benedict J. Kemp, Julia Hatzold, Laura A. Sternick, Joshua Cornman-Homonoff, Jessica M. Whitaker, Pamela J. Tieu, Eric J. Lambie
Dartmouth Scholarship
Homologous recombination provides a means for the in vivoconstruction of recombinant DNA molecules that may be problematic to assemble in vitro . We have investigated the efficiency of recombination within the Caenorhabditis elegans germ line as a function of the length of homology between recombining molecules. Our findings indicate that recombination can occur between molecules that share only 10 bp of terminal homology, and that 25 bp is sufficient to mediate relatively high levels of recombination. Recombination occurs with lower efficiency when the location of the homologous segment is subterminal or internal. As in yeast, recombination can also be …
Scope: A Web Server For Practical De Novo Motif Discovery, Jonathan M. Carlson, Arijit Chakravarty, Charles E. Deziel, Robert H. Gross
Scope: A Web Server For Practical De Novo Motif Discovery, Jonathan M. Carlson, Arijit Chakravarty, Charles E. Deziel, Robert H. Gross
Dartmouth Scholarship
SCOPE is a novel parameter-free method for the de novoidentification of potential regulatory motifs in sets of coordinately regulated genes. The SCOPE algorithm combines the output of three component algorithms, each designed to identify a particular class of motifs. Using an ensemble learning approach, SCOPE identifies the best candidate motifs from its component algorithms. In tests on experimentally determined datasets, SCOPE identified motifs with a significantly higher level of accuracy than a number of other web-based motif finders run with their default parameters. Because SCOPE has no adjustable parameters, the web server has an intuitive interface, requiring only a …