Open Access. Powered by Scholars. Published by Universities.®
- Keyword
-
- Adaptor proteins (1)
- Animals (1)
- Binding sites (1)
- Carrier proteins (1)
- Cell line (1)
-
- Cell nucleolus (1)
- Chromatin (1)
- Deficiency (1)
- Dna-binding proteins (1)
- Genetic (1)
- Hela cells (1)
- High mobility group proteins (1)
- Humans (1)
- Isolation & purification (1)
- Knockout (1)
- Ligands (1)
- Low density lipoprotein receptor-related protein-2 (1)
- Mice (1)
- Myosin heavy chains (1)
- Neuropeptides (1)
- Nuclear proteins (1)
- Pathology (1)
- Phosphoproteins (1)
- Proteinuria (1)
- Ribosomal (1)
- Rna (1)
Articles 1 - 2 of 2
Full-Text Articles in Medicine and Health Sciences
Nucleolin Is Required For Rna Polymerase I Transcription In Vivo, Brendan Rickards, S. Flint, Michael D. Cole, Gary Leroy
Nucleolin Is Required For Rna Polymerase I Transcription In Vivo, Brendan Rickards, S. Flint, Michael D. Cole, Gary Leroy
Dartmouth Scholarship
Eukaryotic genomes are packaged with histones and accessory proteins in the form of chromatin. RNA polymerases and their accessory proteins are sufficient for transcription of naked DNA, but not of chromatin, templates in vitro. In this study, we purified and identified nucleolin as a protein that allows RNA polymerase II to transcribe nucleosomal templates in vitro. As immunofluorescence confirmed that nucleolin localizes primarily to nucleoli with RNA polymerase I, we demonstrated that nucleolin allows RNA polymerase I transcription of chromatin templates in vitro. The results of chromatin immunoprecipitation experiments established that nucleolin is associated with chromatin containing rRNA genes transcribed …
Binding Of Internalized Receptors To The Pdz Domain Of Gipc/Synectin Recruits Myosin Vi To Endocytic Vesicles, Samia N. Naccache, Tama Hasson, Arie Horowitz
Binding Of Internalized Receptors To The Pdz Domain Of Gipc/Synectin Recruits Myosin Vi To Endocytic Vesicles, Samia N. Naccache, Tama Hasson, Arie Horowitz
Dartmouth Scholarship
Myosin VI (myo6) is the only actin-based molecular motor that translocates along actin filaments toward the minus end. Myo6 participates in two steps of endocytic trafficking; it is recruited to both clathrin-coated pits and to ensuing uncoated endocytic vesicles (UCV). Although there is evidence suggesting that the PDZ adaptor protein GIPC/synectin is involved in the association of myo6 with UCV, the recruitment mechanism is unknown. We show that GIPC/synectin is required for both internalization of cell surface receptors and for coupling of myo6 to UCV. This coupling occurs via a mechanism wherein engagement of the GIPC/synectin PDZ domain by C …