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The Product Of The Saccharomyces Cerevisiae Rss1 Gene, Identified As A High-Copy Suppressor Of The Rat7-1 Temperature-Sensitive Allele Of The Rat7/Nup159 Nucleoporin, Is Required For Efficient Mrna Export, Veronica Del Priore, Christine A. Snay, Andre Bahr, Charles N. Cole
The Product Of The Saccharomyces Cerevisiae Rss1 Gene, Identified As A High-Copy Suppressor Of The Rat7-1 Temperature-Sensitive Allele Of The Rat7/Nup159 Nucleoporin, Is Required For Efficient Mrna Export, Veronica Del Priore, Christine A. Snay, Andre Bahr, Charles N. Cole
Dartmouth Scholarship
RAT7/NUP159 was identified previously in a screen for genes whose products are important for nucleocytoplasmic export of poly(A)+ RNA and encodes an essential nucleoporin. We report here the identification of RSS1 (Rat Seven Suppressor) as a high-copy extragenic suppressor of the rat7-1 temperature-sensitive allele. Rss1p encodes a novel essential protein of 538 amino acids, which contains an extended predicted coiled-coil domain and is located both at nuclear pore complexes (NPCs) and in the cytoplasm. RSS1 is the first reported high-copy extragenic suppressor of a mutant nucleoporin. Overexpression of Rss1p partially suppresses the defects in nucleocytoplasmic export of poly(A)+ RNA, rRNA …
Differential Expression Of The Toxr Regulon In Classical And E1 Tor Biotypes Of Vibrio Cholerae Is Due To Biotype-Specific Control Over Toxt Expression., Victor J. Dirita, Melody Neely, Ronald K. Taylor, Paul M. Bruss
Differential Expression Of The Toxr Regulon In Classical And E1 Tor Biotypes Of Vibrio Cholerae Is Due To Biotype-Specific Control Over Toxt Expression., Victor J. Dirita, Melody Neely, Ronald K. Taylor, Paul M. Bruss
Dartmouth Scholarship
The two major disease-causing biotypes of Vibrio cholerae, classical and El Tor, exhibit differences in their epidemic nature. Their behavior in the laboratory also differs in that El Tor strains produce two major virulence factors, cholera toxin (CT) and the toxin coregulated pilus (TCP), only under very restricted growth conditions, whereas classical strains do so in standard laboratory medium. Expression of toxin and TCP is controlled by two activator proteins, ToxR and ToxT, that operate in cascade fashion with ToxR controlling the synthesis of ToxT. Both biotypes express equivalent levels of ToxR, but only classical strains appear to express ToxT …