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Full-Text Articles in Medicine and Health Sciences
Attachment Of Toxoplasma Gondii To A Specific Membrane Fraction Of Cho Cells, Chaitali Dutta, Jane Grimwood, Lloyd H. Kasper
Attachment Of Toxoplasma Gondii To A Specific Membrane Fraction Of Cho Cells, Chaitali Dutta, Jane Grimwood, Lloyd H. Kasper
Dartmouth Scholarship
We have observed previously that attachment of Toxoplasma gondii to synchronized host cells is considerably increased at the mid-S phase (4 h postrelease). Synchronized CHO host cells at the mid-S phase were fractionated by molecular weight, and the antigens were used to produce a panel of polyclonal mouse antisera. The polyclonal antisera raised against fraction 4 with molecular mass ranging approximately from 18 to 40 kDa significantly reduced attachment to mid-S-phase host cells. Immunofluorescence assays demonstrated strong reactivity to mid-S-phase host cells and identified a number of potential receptors on Western blots. These data indicate that there is a specific …
Lack Of Cd4+ T Cells Does Not Affect Induction Of Cd8+ T-Cell Immunity Against Encephalitozoon Cuniculi Infection, Magali Moretto, Lori Casciotti, Brigit Durell, Imtiaz A. Khan
Lack Of Cd4+ T Cells Does Not Affect Induction Of Cd8+ T-Cell Immunity Against Encephalitozoon Cuniculi Infection, Magali Moretto, Lori Casciotti, Brigit Durell, Imtiaz A. Khan
Dartmouth Scholarship
Cell-mediated immunity has been reported to play an important role in defense against Encephalitozoon cuniculi infection. Previous studies from our laboratory have underlined the importance of cytotoxic CD8+ T lymphocytes (CTL) in survival of mice infected with E. cuniculi. In the present study, immune response against E. cuniculi infection in CD4+T-cell-deficient mice was evaluated. Similar to resistant wild-type animals, CD4−/− mice were able to resolve E. cuniculi infection even at a very high challenge dose (5 × 107 spores/mouse). Tissues from infected CD4−/−mice did not exhibit higher parasite loads in comparison to …
Staphylococcus Aureus Rn6390 Replicates And Induces Apoptosis In A Pulmonary Epithelial Cell Line, Barbara C. Kahl, Mark Goulian, Willem Van Wamel, Mathias Herrmann, Sanford M. Simon, Gilla Kaplan, Georg Peters, Ambrose L. Cheung
Staphylococcus Aureus Rn6390 Replicates And Induces Apoptosis In A Pulmonary Epithelial Cell Line, Barbara C. Kahl, Mark Goulian, Willem Van Wamel, Mathias Herrmann, Sanford M. Simon, Gilla Kaplan, Georg Peters, Ambrose L. Cheung
Dartmouth Scholarship
Staphylococcus aureus frequently colonizes the airways of patients with compromised airway defenses (e.g., cystic fibrosis [CF] patients) for extended periods. Persistent and relapsing infections may be related to live S. aureus bacteria actively residing inside epithelial cells. In this study, we infected a respiratory epithelial cell line, which was derived from a CF patient, with S. aureus RN6390. Internalization of S. aureus was found to be time and dose dependent and could be blocked by cytochalasin D. Transmission electron microscopy revealed that internalized bacteria resided within endocytic vacuoles without any evidence of lysosomal fusion in a 24-h period. The results …
Differential Infectivity And Division Of Toxoplasma Gondii In Human Peripheral Blood Leukocytes, Jacqueline Y. Channon, Rosanne M. Seguin, Lloyd H. Kasper
Differential Infectivity And Division Of Toxoplasma Gondii In Human Peripheral Blood Leukocytes, Jacqueline Y. Channon, Rosanne M. Seguin, Lloyd H. Kasper
Dartmouth Scholarship
When tachyzoites were incubated with human peripheral blood leukocytes in vitro, more monocytes and dendritic cells than neutrophils or lymphocytes were infected. Although tachyzoites were able to divide in each of these cell types, monocytes and dendritic cells were more permissive to rapid tachyzoite division than neutrophils or lymphocytes.
Effects Of Estradiol And Progesterone On Susceptibility And Early Immune Responses To Chlamydia Trachomatis Infection In The Female Reproductive Tract, Charu Kaushic, Fan Zhou, Andrew D. Murdin, Charles R. Wira
Effects Of Estradiol And Progesterone On Susceptibility And Early Immune Responses To Chlamydia Trachomatis Infection In The Female Reproductive Tract, Charu Kaushic, Fan Zhou, Andrew D. Murdin, Charles R. Wira
Dartmouth Scholarship
We have used a previously described rodent model to examine the influence of hormonal environment on susceptibility and immune responses to genital Chlamydia infection. Ovariectomized rats were administered estradiol, progesterone, or a combination of both, infected with Chlamydia trachomatis via the intrauterine route, and sacrificed 5 days later. Histopathological examination showed severe inflammation in the uteri and vaginae of progesterone-treated animals, whereas animals receiving estradiol or a combination of both hormones showed no inflammation. Large numbers of chlamydiae were found in vaginal secretions of progesterone-treated and combination-treated animals, while estradiol-treated animals had none. Tissue localization showed that numerous chlamydial inclusions …