Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 1 of 1
Full-Text Articles in Medicine and Health Sciences
Crosstalk Between Adipocytes And Immune Cells In Adipose Tissue In An Obese Inflammatory State: Role Of Contact-Mediated Signaling, Carolina Franco Nitta
Crosstalk Between Adipocytes And Immune Cells In Adipose Tissue In An Obese Inflammatory State: Role Of Contact-Mediated Signaling, Carolina Franco Nitta
Biomedical Sciences ETDs
Obesity is defined as heightened fat accumulation leading to health impairments. It has been directly correlated to cardiovascular disease, type II diabetes mellitus, and cancer. Heightened cytokine levels are found in serum and adipose tissue of obese subjects, including TNFα (tumor necrosis factor alpha), IL-6 (interleukin-6), and MCP-1 (monocyte chemoattractant protein-1), being characterized as a chronic low-grade inflammatory disease. In this dissertation, I have generated a novel co-culture model between adipocytes and immune cells (derived from splenocytes) that mimics inflammation seen in obese adipose tissue. This co-culture model allows for distinct evaluation between secreted paracrine factors (indirect cultures) and these …