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Full-Text Articles in Medicine and Health Sciences
Pancreatic Function In Cystic Fibrosis: As Measured In The Chronically Reserpinized Rabbit Model, Mitchell L. Shiffman
Pancreatic Function In Cystic Fibrosis: As Measured In The Chronically Reserpinized Rabbit Model, Mitchell L. Shiffman
Biomedical Sciences ETDs
Pancreatic juice was collected from rabbits treated for seven days with reserpine and from untreated controls. In the treated animals the volume of pancreatic juice secreted, the flow rate and the bicarbonate output were all significantly less, while the total protein concentration, the amylase activity and the calcium concentration of the juice were all significantly elevated. This elevation in the protein concentration was due to increased amounts of protein and decreased amounts of water. Histological sections of the pancreas of treated animals revealed the presence of large amounts of PAS-positive material within the duct lumen. Since these secretory and morphological …
The Mcleod Erythrocyte Membrane: Freeze Fracture Electron Microscopy And Membrane Protein Electrophoresis, Carolyn S. Glaubensklee
The Mcleod Erythrocyte Membrane: Freeze Fracture Electron Microscopy And Membrane Protein Electrophoresis, Carolyn S. Glaubensklee
Biomedical Sciences ETDs
The McLeod erythrocyte is characterized by a weak Kell antigenicity, lack of Kx antigen, and an abnormal cell morphology. These properties are shared by the prepositus, Hugh McLeod, and male children afflicted by type II chronic granulomatous disease. X-linked chronic granulomatous disease involves the inability of affected leukocytes to digest phagocytized bacteria leading to premature death. Prior studies have shown the McLeod erythrocyte membrane to be not only antigenically abnormal, but also exhibit an abnormal acanthocytic shape and decreased water permeability. These properties were found to be independent of the membrane phospholipid composition, cholesterol/phospholipid ratio, and microviscosity. The decreased water …
Erythrocyte Transketolase Activity Measurements: Assessment Of Thiamine Status In Rat And Man, Marilyn Thomas Milchanowski
Erythrocyte Transketolase Activity Measurements: Assessment Of Thiamine Status In Rat And Man, Marilyn Thomas Milchanowski
Biomedical Sciences ETDs
An individual's thiamine status can be assessed by the measurement of erythrocyte transketolase (ETK) activity and a new method for this type of measurement in rat and human whole blood hemolysate samples has been developed and completely automated. This assay is based on enzymatic activity of the hexose monophosphate shunt which involves interconversion of hexoses and pentoses and production of NADPH reducing power. All of the enzymes of the hexose monophosphate shunt (HMS) are present in erythrocytes, including transketolase (TK) which appears to be the rate-Iimiting enzyme in this reaction series. Reaction conditions are established to ensure a direct relationship …