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Full-Text Articles in Medicine and Health Sciences
Cytolytic And Ion Channel Forming Properties Of The N-Terminus Of Lymphocyte Perforin, David M. Ojcius, Pedro M. Persechini, Li-Mou Zheng, Paulo C. Notaroberto, Sandro C. Adeodato, John Ding-E. Young
Cytolytic And Ion Channel Forming Properties Of The N-Terminus Of Lymphocyte Perforin, David M. Ojcius, Pedro M. Persechini, Li-Mou Zheng, Paulo C. Notaroberto, Sandro C. Adeodato, John Ding-E. Young
All Dugoni School of Dentistry Faculty Articles
Perforin lyses cells by binding to the target cell membrane, where it polymerizes into large nonspecific pores. It is shown here that the first 34 amino acids of the N-terminal region of either human or murine perforin are soluble in aqueous medium and spontaneously insert into membranes. The N-terminal peptides lyse liposomes and nucleated cells, and they form ion channels in planar bilayers, some of which are comparable to those previously described for perforin. The lytic activity of the N-terminal domains does not require calcium, is independent of the lipid headgroup composition, and can be inhibited by heparin. Tumor cells …
Enhancement Of Human Immunodeficiency Virus Type 1 Infection By Cationic Liposomes: The Role Of Cd4, Serum And Liposome-Cell Interactions, Krystyna Konopka, L. L. Stamatatos, C. E. Larsen, B. R. Davis, Nejat Düzgüneş
Enhancement Of Human Immunodeficiency Virus Type 1 Infection By Cationic Liposomes: The Role Of Cd4, Serum And Liposome-Cell Interactions, Krystyna Konopka, L. L. Stamatatos, C. E. Larsen, B. R. Davis, Nejat Düzgüneş
All Dugoni School of Dentistry Faculty Articles
We have reported previously the enhancement of the infectivity of human immunodeficiency virus type 1 (HIV- 1) by liposomes composed of the cationic lipid N[2,3-(dioleyloxy) propyl]-N,N,N-trimethylammonium chloride (DOTMA). To determine the mechanism by which this process occurs, we have investigated the role of CD4, serum concentration and liposome-cell interactions in the DOTMA-mediated stimulation of HIV-1 infection of A3.01 cells. Serum alone significantly inhibited the binding and infectivity of HIV-1, but DOTMA-mediated enhancement of infectivity was more pronounced in the presence of serum than in its absence. HIV-1 binding to cells was increased in the presence of DOTMA liposomes, DEAE-dextran and …