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Chemiosmotic Properties Of Isolated Secretory Granules From Parotid Glands Of Normal And Isoproterenol Treated Rats, Peter Raymond Arvan
Chemiosmotic Properties Of Isolated Secretory Granules From Parotid Glands Of Normal And Isoproterenol Treated Rats, Peter Raymond Arvan
Yale Medicine Thesis Digital Library
Secretory granule fractions were prepared isoosmotically from the parotid glands of normal rats and from rats that had received repeated injections of isoproterenol. These granules, purified extensively, are stable after isolation, making it possible to study biophysical parameters which may relate to their role in intracellular transport and secretion.Normal parotid granules have an internal pH of (TURN)6.8 and a low ionic permeability with respect to internal buffering capacity. By contrast, granules from the treated rats have an internal pH ranging from (TURN)7.7 in (0.3 M) sucrose media to (TURN)7.1 in (100 mM) KCl media; suggesting a large ionic permeability with …