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Full-Text Articles in Medicine and Health Sciences
Characterization Of Inhbb, Heatr5a, & Cyp2s1 Expression In Dorsal Root Ganglia By In-Situ Hybridization, Joshua D. Krech
Characterization Of Inhbb, Heatr5a, & Cyp2s1 Expression In Dorsal Root Ganglia By In-Situ Hybridization, Joshua D. Krech
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Multiple studies have shown that gene expression changes occur in sensory neurons after peripheral nerve injury (PNI). These expression changes include many genes that are turned on specifically in response to injury, but much less is know about expression changes in stable genetic markers of particular sensory neuron populations. This study characterized the expression of three markers of proprioceptive neurons Inhbb, Heatr5a, Cyp2s1 in lumbar dorsal root ganglion (DRG) neurons in intact animals and after PNI. To perform these experiments, we subcloned segments of the coding sequences of these genes and generated DIG-labeled riboprobes. Control experiments demonstrated the validity of …
Characterization Of B3galt2 And Heg1 Expression In Dorsal Root Ganglia, Alexander H. Nguyen
Characterization Of B3galt2 And Heg1 Expression In Dorsal Root Ganglia, Alexander H. Nguyen
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Proprioception provides unique sensory feedback about the body’s orientation in space and this sensation is essential for creating specific motor functions and coordinated movement. During neurogenesis, unique genetic markers are expressed and classified as early, transient/middle, and late markers depending on their timing of expression. This study focuses on the expression patterns of two late markers: Heg1 and B3galt2. We investigated how expression of these two markers respond to peripheral nerve injury (PNI). We cloned and generated riboprobes to detect expression of B3galt2 and Heg1 in DRGs using in situ hybridization after transection of the sciatic nerve. B3galt2 hybridization in …
C-Bouton Coverage Of Alpha-Motoneurons Following Peripheral Nerve Injury, Esra Salah Shermadou
C-Bouton Coverage Of Alpha-Motoneurons Following Peripheral Nerve Injury, Esra Salah Shermadou
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Peripheral nerve injuries (PNI) cause alternations in central synapses leading to loss of function. The C-bouton synapses onto a-motoneurons in the ventral horn, and has a role in regulating motor output. Following tibial nerve ligation, the somatic C-bouton coverage is depleted (Alvarez et al., 2011), however, it is unknown what happens following crush type injuries. PNI causes neuroglia activation and proliferation that contribute to synaptic alterations, a response that has not been well-characterized in the ventral horn, where motoneurons are located. Therefore, I hypothesize that glia activation following peripheral nerve injury correlates to the degree of depletion of synaptic coverage …