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Developmental Expression Of Calcium Buffering Proteins In Central Auditory Pathways Of Normal Hearing And Congenitally Deaf Mice, Adam S. Deardorff
Developmental Expression Of Calcium Buffering Proteins In Central Auditory Pathways Of Normal Hearing And Congenitally Deaf Mice, Adam S. Deardorff
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These experiments analyze differences in synaptic development in central auditory pathways between normal hearing (CBA/J) and congenitally deaf (dn/dn) mice, which provide valuable insight into central synaptic plasticity corresponding to human congenital deafness. Immunofluorescent analysis of the developmental expression of the calcium buffering proteins calretinin, calbindin d-28k, and parvalbumin at various postnatal time points was performed to assess the effects of altered neural activity on the level and/or pattern of protein expression within these nuclei. Results indicate that the pattern of calbindin and parvalbumin is unaffected by congenital deafness in dn/dn mice. However, the pattern of calretinin expression in the …
Developmental Expression Of Calcium-Binding Proteins In The Avcn And Mntb Of Normal Hearing And Congenitally Deaf Mice, John L. Roebel
Developmental Expression Of Calcium-Binding Proteins In The Avcn And Mntb Of Normal Hearing And Congenitally Deaf Mice, John L. Roebel
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This experiment analyzes synaptic differences in the central auditory pathway between normal hearing and congenitally deaf (dn/dn) mice, and provides valuable insight into central changes that correspond with human congenital deafness. Specifically, this experiment analyzes developmental expression of the Calcium (Ca2+)-binding proteins Calretinin (CR), Calbindin D-28k (CB) and Parvalbumin (PV) in large excitatory synapses in the anteroventral cochlear nucleus (AVCN) and the medial nucleus of the trapezoid body (MNTB) of normal and dn/dn mice. Immunofluorescence imaging with primary antibodies detecting CR, CB or PV was used to analyze the expression of each at 9 days, 13 days, 20 days, 30 …