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Characterization Of Inhbb, Heatr5a, & Cyp2s1 Expression In Dorsal Root Ganglia By In-Situ Hybridization, Joshua D. Krech
Characterization Of Inhbb, Heatr5a, & Cyp2s1 Expression In Dorsal Root Ganglia By In-Situ Hybridization, Joshua D. Krech
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Multiple studies have shown that gene expression changes occur in sensory neurons after peripheral nerve injury (PNI). These expression changes include many genes that are turned on specifically in response to injury, but much less is know about expression changes in stable genetic markers of particular sensory neuron populations. This study characterized the expression of three markers of proprioceptive neurons Inhbb, Heatr5a, Cyp2s1 in lumbar dorsal root ganglion (DRG) neurons in intact animals and after PNI. To perform these experiments, we subcloned segments of the coding sequences of these genes and generated DIG-labeled riboprobes. Control experiments demonstrated the validity of …
The Effect Of Endogenous Ligands Of The Aryl Hydrocarbon Receptor On Antibody Expression In A Human B-Cell Model, Valerie Benedict
The Effect Of Endogenous Ligands Of The Aryl Hydrocarbon Receptor On Antibody Expression In A Human B-Cell Model, Valerie Benedict
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Small changes in antibody expression have broad impacts on human health and disease. An environmental toxicant 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) induces profound immune suppression in animal models in an aryl hydrocarbon receptor (AhR)-mediated fashion. In a human Burkitt's lymphoma cell line (CL-01), TCDD inhibited IgG secretion and differentially impacted Ig heavy chain expression. In addition to TCDD, the AhR has several endogenous, dietary, and bacterial ligands such as 6-formylindolo[3,2-b]carbazole (FICZ) and indole, which are present within the human body at varied concentrations (0.30 mM- 6.64 mM). Both FICZ (100 nM) and indole (100 µM) significantly reduced IgG secretion. Overall antibody secretion is …