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Full-Text Articles in Medicine and Health Sciences
In Vivo Extracellular Ph Mapping Of Tumors Using Electron Paramagnetic Resonance, Denis A. Komarov, Yuki Ichikawa, Kumiko Yamamoto, Neil J. Stewart, Shingo Matsumoto, Hironobu Yasui, Igor A. Kirilyuk, Valery V. Khramtsov, Osamu Inanami, Hiroshi Hirata
In Vivo Extracellular Ph Mapping Of Tumors Using Electron Paramagnetic Resonance, Denis A. Komarov, Yuki Ichikawa, Kumiko Yamamoto, Neil J. Stewart, Shingo Matsumoto, Hironobu Yasui, Igor A. Kirilyuk, Valery V. Khramtsov, Osamu Inanami, Hiroshi Hirata
Clinical and Translational Science Institute
An electron paramagnetic resonance (EPR)- based method for noninvasive three-dimensional extracellular pH mapping was developed using a pH-sensitive nitroxyl radical as an exogenous paramagnetic probe. Fast projection scanning with a constant magnetic field sweep enabled the acquisition of four-dimensional (3D spatial +1D spectral) EPR images within 7.5 min. Three-dimensional maps of pH were reconstructed by processing the pH-dependent spectral information on the images. To demonstrate the proposed method of pH mapping, the progress of extracellular acidosis in tumor-bearing mouse legs was studied. Furthermore, extracellular pH mapping was used to visualize the spatial distribution of acidification in different tumor xenograft mouse …
In Vivo Electron Paramagnetic Resonance: Radical Concepts For Translation To The Clinical Setting, Valery V. Khramtsov
In Vivo Electron Paramagnetic Resonance: Radical Concepts For Translation To The Clinical Setting, Valery V. Khramtsov
Clinical and Translational Science Institute
Electron paramagnetic resonance (EPR)-based spectroscopic and imaging techniques allow for the study of free radicals—molecules with one or more unpaired electrons. Biological EPR applications include detection of endogenous biologically relevant free radicals as well as use of specially designed exogenous radicals to probe local microenvironments. This Forum focuses on recent advances in the field of in vivo EPR applications discussed at the International Conference on Electron Paramagnetic Resonance Spectroscopy and Imaging of Biological Systems (EPR-2017). Although direct EPR detection of endogenous free radicals such as reactive oxygen species (ROS) in vivo remains unlikely in most cases, alternative approaches based on …
In Vivo Molecular Electron Paramagnetic Resonance-Based Spectroscopy And Imaging Of Tumor Microenvironment And Redox Using Functional Paramagnetic Probes, Valery V. Khramtsov
In Vivo Molecular Electron Paramagnetic Resonance-Based Spectroscopy And Imaging Of Tumor Microenvironment And Redox Using Functional Paramagnetic Probes, Valery V. Khramtsov
Clinical and Translational Science Institute
Significance: A key role of the tumor microenvironment (TME) in cancer progression, treatment resistance, and as a target for therapeutic intervention is increasingly appreciated. Among important physiological components of the TME are tissue hypoxia, acidosis, high reducing capacity, elevated concentrations of intracellular glutathione (GSH), and interstitial inorganic phosphate (Pi). Noninvasive in vivo pO2, pH, GSH, Pi, and redox assessment provide unique insights into biological processes in the TME, and may serve as a tool for preclinical screening of anticancer drugs and optimizing TME-targeted therapeutic strategies. Recent Advances: A reasonable radiofrequency penetration depth in living tissues and progress in development of …
In Vivo Epr Assessment Of Ph, Po2, Redox Status, And Concentrations Of Phosphate And Glutathione In The Tumor Microenvironment, Andrey A. Bobko, Timothy D. Eubank, Benoit Driesschaert, Valery V. Khramtsov
In Vivo Epr Assessment Of Ph, Po2, Redox Status, And Concentrations Of Phosphate And Glutathione In The Tumor Microenvironment, Andrey A. Bobko, Timothy D. Eubank, Benoit Driesschaert, Valery V. Khramtsov
Clinical and Translational Science Institute
This protocol demonstrates the capability of low-field electron paramagnetic resonance (EPR)-based techniques in combination with functional paramagnetic probes to provide quantitative information on the chemical tumor microenvironment (TME), including pO2, pH, redox status, concentrations of interstitial inorganic phosphate (Pi), and intracellular glutathione (GSH). In particular, an application of a recently developed soluble multifunctional trityl probe provides unsurpassed opportunity for in vivo concurrent measurements of pH, pO2 and Pi in Extracellular space (HOPE probe). The measurements of three parameters using a single probe allow for their correlation analyses independent of probe distribution and time of the measurements.