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Full-Text Articles in Medicine and Health Sciences
Identification Of Novel Regulatory Cholesterol Metabolite, 5-Cholesten, 3Β,25-Diol, Disulfate, Shunlin Ren, Jin Koung Kim, Genta Kakiyama, Daniel Rodriguez-Agudo, William M. Pandak, Hae-Ki Min, Yanxia Ning
Identification Of Novel Regulatory Cholesterol Metabolite, 5-Cholesten, 3Β,25-Diol, Disulfate, Shunlin Ren, Jin Koung Kim, Genta Kakiyama, Daniel Rodriguez-Agudo, William M. Pandak, Hae-Ki Min, Yanxia Ning
VCU Health Publications
Oxysterol sulfation plays an important role in regulation of lipid metabolism and inflammatory responses. In the present study, we report the discovery of a novel regulatory sulfated oxysterol in nuclei of primary rat hepatocytes after overexpression of the gene encoding mitochondrial cholesterol delivery protein (StarD1). Forty-eight hours after infection of the hepatocytes with recombinant StarD1 adenovirus, a water-soluble oxysterol product was isolated and purified by chemical extraction and reverse-phase HPLC. Tandem mass spectrometry analysis identified the oxysterol as 5-cholesten-3β, 25-diol, disulfate (25HCDS), and confirmed the structure by comparing with a chemically synthesized compound. Administration of 25HCDS to human THP-1-derived macrophages …
Clinical And Microbiologic Efficacy Of A Water Filter Program In A Rural Honduran Community, Jaclyn Arquiette, Michael P. Stevens, Jean M. Rabb, Kakotan Sanogo, Patrick Mason, Gonzalo Bearman
Clinical And Microbiologic Efficacy Of A Water Filter Program In A Rural Honduran Community, Jaclyn Arquiette, Michael P. Stevens, Jean M. Rabb, Kakotan Sanogo, Patrick Mason, Gonzalo Bearman
VCU Health Publications
Water purification in the rural Honduras is a focus of the nonprofit organization Honduras Outreach Medical Brigade Relief Effort (HOMBRE). We assessed water filter use and tested filter microbiologic and clinical efficacy. A 22-item questionnaire assessed water sources, obtainment/storage, purification, and incidence of gastrointestinal disease. Samples from home clay-based filters in La Hicaca were obtained and paired with surveys from the same home. We counted bacterial colonies of four bacterial classifications from each sample. Sixty-five surveys were completed. Forty-five (69%) individuals used a filter. Fifteen respondents reported diarrhea in their home in the last 30 days; this incidence was higher …
Abnormal Downward Gaze And Cold Caloric Examination Due To Propofol: A Case Study, Thuy-Anh Vu, Alia O'Meara, Jean Teasley
Abnormal Downward Gaze And Cold Caloric Examination Due To Propofol: A Case Study, Thuy-Anh Vu, Alia O'Meara, Jean Teasley
VCU Health Publications
Background: Adolescent patient with isolated abnormal downward gaze and oculovestibular (cold caloric) testing during propofol administration prompting concern of brainstem injury. PATIENT: An otherwise healthy 16 year old female presented after an intentional hanging. Brainstem reflexes were normal except that both eyes were in tonic downgaze on initial examination. Propofol was suspended for 30 minutes to evaluate level of responsiveness with eyes normalizing to midposition from tonic downgaze. With reinitiation of propofol, the eyes returned to the former position. C-collar stabilization prohibited oculocephalic (doll’s eyes) evaluation, and with cold caloric testing, eye movements were as follows: right sided cold water …