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Dengue Virus Pathogenesis In Mouse Central Nervous System: Studies On Host Response To Dengue Virus Infection, Juliano Bordignon, Christian Probst, Ana Luiza P. Mosimann, Vanessa Stella, Daniela Pavoni, Gregory A. Buck, Paul Fawcett, Silvio M. Zanata, Lucia Noronha, Marco Krieger, Claudia Nunes Duarte Dos Santos

Microbiology and Immunology Publications

Dengue virus (DENV) causes a self-limiting fever (DF) or severe hemorrhagic fever/shock-syndrome (DHF/DSS). Recently, clinical profile of DENV infection is changing, and neurological manifestations are becoming frequent. We previously demonstrated that mutations on E and NS3 proteins may account for DENV neurovirulence for mice. To validate the involvement of the observed mutations in the appearance of neurovirulent viral phenotypes, we constructed cDNA infectious clones encompassing the observed mutations. Aiming to determine host response to infection with neurovirulent and parental strains of DENV-1, we used a Mus musculus biochip (Virginia Commonwealth University) encompassing whole mouse genome. Newborn Swiss mice were infected …