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Full-Text Articles in Medicine and Health Sciences
Delivery Of An Anti-Hiv-1 Ribozyme Into Hiv-Infected Cells Via Cationic Liposomes, Krystyna Konopka, John J. Rossi, Piotr Swiderski, Vladimir A. Slepushkin, Nejat Düzgüneş
Delivery Of An Anti-Hiv-1 Ribozyme Into Hiv-Infected Cells Via Cationic Liposomes, Krystyna Konopka, John J. Rossi, Piotr Swiderski, Vladimir A. Slepushkin, Nejat Düzgüneş
All Dugoni School of Dentistry Faculty Articles
Cationic liposome-mediated intracellular delivery of a fluorescein-labeled chimeric DNA-RNA ribozyme targeted to the HIV-1 5' LTR was investigated, using THP-1, THP-1/HIV-1(IIIB) or HeLa/LAV cells. Different fluorescence patterns were observed when the cells were exposed to Lipofectamine, Lipofectin or DMRIE:DOPE (1:1) complexed to the ribozyme. With Lipofectamine intense cell-associated fluorescence was found. Incubation with Lipofectin resulted in less intense diffuse fluorescence, while with DMRIE an intense but sporadic fluorescence was observed. Differentiated THP-1/HIV-1(IIIB) cells were more susceptible to killing by liposome-ribozyme complexes than THP-1 cells. Under non-cytotoxic conditions (a 4-h treatment) complexes of 5, 10 or 15 μM Lipofectin or DOTAP:DOPE …
Enhancement Of Atp Levels And Glucose Metabolism During An Infection By Chlamydia: Nmr Studies Of Living Cells, David M. Ojcius, H. Degani, Joel Mispelter, Alice Dautry-Varsat
Enhancement Of Atp Levels And Glucose Metabolism During An Infection By Chlamydia: Nmr Studies Of Living Cells, David M. Ojcius, H. Degani, Joel Mispelter, Alice Dautry-Varsat
All Dugoni School of Dentistry Faculty Articles
The Chlamydia species are obligate intracellular bacteria that proliferate only within the infected cell. Since the extracellular bacteria are metabolically inert and there are no cell-free systems for characterizingChlamydia metabolism, we studied metabolic changes related to ATP synthesis and glycolysis in HeLa cells infected withChlamydia psittaci during the course of the 2-day infection cycle using noninvasive 31P and 13C NMR methods. We find that the infection stimulates ATP synthesis in the infected cell, with a peak of ATP levels occurring midway through the infection cycle, when most of the metabolically active bacteria are proliferating. The infection also stimulates synthesis of …
The Effect Of Sinoaortic Deafferentation On Renal Wrap Hypertension, J. Mark Van Ness, Teresa Craig, Carmen Hinojosa-Laborde, Joseph R. Haywood
The Effect Of Sinoaortic Deafferentation On Renal Wrap Hypertension, J. Mark Van Ness, Teresa Craig, Carmen Hinojosa-Laborde, Joseph R. Haywood
College of the Pacific Faculty Articles
The purpose of this study was to determine whether sinoaortic deafferentation (SAD) alters the severity of hypertension or sympathoadrenal contribution to mean blood pressure (MAP) during renal wrap hypertension. Male Sprague-Dawley rats were implanted with radiotelemetry transmitters for 24-hour recording of MAP and heart rate. All rats underwent either SAD or sham SAD (Intact) surgery and were allowed to recover for 10 to 14 days. The rats were then assigned to a normotensive (Sham) group or a hypertensive (Wrap) group in which 1-kidney figure-8 renal wrap was performed. SAD increased the acute MAP response to renal wrap (Intact-Sham=5+/-1 mm Hg, …
Gene Delivery By Negatively Charged Ternary Complexes Of Dna, Cationic Liposomes And Transferrin Or Fusigenic Peptides, Sérgio Simões, Vladimir Slepushkin, R. Caspar, Maria C. Pedroso De Lima, Nejat Düzgüneş
Gene Delivery By Negatively Charged Ternary Complexes Of Dna, Cationic Liposomes And Transferrin Or Fusigenic Peptides, Sérgio Simões, Vladimir Slepushkin, R. Caspar, Maria C. Pedroso De Lima, Nejat Düzgüneş
All Dugoni School of Dentistry Faculty Articles
Potential problems with the use of viral vectors for gene therapy necessitate the development of efficient nonviral vectors. The association of transferrin, or the pH-sensitive peptide GALA, with cationic liposomes composed of 1,2-dioleoyl-3-(trimethylammonium) propane and its equimolar mixture with dioleoylphosphatidylethanolamine, under conditions where the liposome/DNA complex is negatively charged, drastically increased luciferase expression from pCMVluc. The percentage of cells transfected, measured by β-galactosidase expression, was also increased by about 10-fold. The zeta potential of the ternary complexes was lower than that of the liposome/DNA complexes. Transfection activity of positively charged complexes was also enhanced by association with transferrin, GALA or …