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Full-Text Articles in Medicine and Health Sciences
Human Lens Lipids Differ Markedly From Those Of Commonly Used Experimental Animals, Jane Deeley, Todd W. Mitchell, Xiaojia Wei, Jurgen Korth, Jessica Hughes, Stephen J. Blanksby, Roger J. Truscott
Human Lens Lipids Differ Markedly From Those Of Commonly Used Experimental Animals, Jane Deeley, Todd W. Mitchell, Xiaojia Wei, Jurgen Korth, Jessica Hughes, Stephen J. Blanksby, Roger J. Truscott
Faculty of Health and Behavioural Sciences - Papers (Archive)
Electrospray ionisation tandem mass spectrometry has allowed the unambiguous identification andquantification of individual lens phospholipids in human and six animal models. Using this approach ca. 100unique phospholipids have been characterised. Parallel analysis of the same lens extracts by a novel directinsertionelectron-ionization technique found the cholesterol content of human lenses to be significantlyhigher (ca. 6 times) than lenses from the other animals.The most abundant phospholipids in all the lenses examined were choline-containing phospholipids. In rat,mouse, sheep, cow, pig and chicken, thesewere present largely as phosphatidylcholines, in contrast 66% of thetotal phospholipid in Homo sapienswas sphingomyelin, with the most abundant being dihydrosphingomyelins,in …
Bovine Feces From Animals With Gastrointestinal Infections Are A Source Of Serologically Diverse Atypical Enteropathogenic Escherichia Coli And Shiga Toxin-Producing E. Coli Strains That Commonly Possess Intimin, Michael A Hornitzky, Kim Mercieca, Karl A Bettelheim, Steven P. Djordjevic
Bovine Feces From Animals With Gastrointestinal Infections Are A Source Of Serologically Diverse Atypical Enteropathogenic Escherichia Coli And Shiga Toxin-Producing E. Coli Strains That Commonly Possess Intimin, Michael A Hornitzky, Kim Mercieca, Karl A Bettelheim, Steven P. Djordjevic
Faculty of Science, Medicine and Health - Papers: part A
Shiga toxin-producing Escherichia coli (STEC) and enteropathogenic E. coli (EPEC) cells were isolated from 191 fecal samples from cattle with gastrointestinal infections (diagnostic samples) collected in New South Wales, Australia. By using a multiplex PCR, E. coli cells possessing combinations of stx1, stx2, eae, and ehxA were detected by a combination of direct culture and enrichment in E. coli (EC) (modified) broth followed by plating on vancomycin-cefixime-cefsulodin blood (BVCC) agar for the presence of enterohemolytic colonies and on sorbitol MacConkey agar for the presence of non-sorbitol-fermenting colonies. The high prevalence of the intimin gene eae …