Medicine and Health Sciences Commons^™

Fgf-2 Counteracts Loss Of Tgfβ Affected Cells From Rat Lens Explants: Implications For Pco (After Cataract), Kylie Mansfield, Anna Cerra, Coral Chamberlain

Faculty of Science, Medicine and Health - Papers: part A

Purpose: While cataract surgery initially benefits most patients, many suffer secondary loss of vision because of posterior capsule opacification (PCO). Lens epithelial cells left behind at surgery become aberrant and migrate into the light path. TGF-beta (TGFβ) appears to play a key role in this process by inducing the cells to undergo an epithelial-mesenchymal transition. Paradoxically, it also typically induces them to undergo apoptotic death. The present study was undertaken to investigate the hypothesis that FGF plays a role in PCO formation by promoting the survival of abnormal cells with PCO-like characteristics.

Methods: Rat lens epithelial explants were cultured for …

A Mixed-Model Approach For The Analysis Of Cdna Microarray Gene Expression Data From Extreme-Performing Pigs After Infection With Actinobacillus Pleuropneumoniae, R J. Moser, A Reverter, Caroline A. Kerr, K J. Beh

Graduate School of Medicine - Papers (Archive)

We proposed a novel statistical approach for the analysis of cDNA experiments based on mixed-model methodology combined with mixtures of distributions. Our objective was to detect genes that may be involved in conferring heritable differences in susceptibility to common infections in intensive pig production. We employed a microarray expression profiling strategy and a mixed-model approach to the analysis of the expression data. A cDNA microarray of pig with 6,420 probes from immune tissues and cells was used to compare gene expression in peripheral blood leukocytes of two pigs showing extreme performance in their response to infection with Actinobacillus pleuropneumoniae. Principal …