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- Whey Protein (5)
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- Allergens -- therapeutic use; Rhinitis (2)
- Allergic -- drug therapy; Sublingual Immunotherapy; Treatment Outcome; Comparative Study (2)
- Asymptomatic sensitization (2)
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- Neuroinflammation (2)
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- A. Muciniphila (1)
- Akkermansia muciniphila (1)
- Allergic Asthma (1)
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- Allergies (1)
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- Asthma (1)
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Articles 1 - 18 of 18
Full-Text Articles in Medicine and Health Sciences
Hla-Ii Alleles Influence Physical And Behavioral Responses To A Whey Allergen In A Transgenic Mouse Model Of Cow's Milk Allergy, Danielle L. Germundson, Suba Nookala, Nicholas A. Smith, Yassmine Warda, Kumi Nagamoto-Combs
Hla-Ii Alleles Influence Physical And Behavioral Responses To A Whey Allergen In A Transgenic Mouse Model Of Cow's Milk Allergy, Danielle L. Germundson, Suba Nookala, Nicholas A. Smith, Yassmine Warda, Kumi Nagamoto-Combs
Biomedical Sciences Faculty Publications
The symptoms of food allergies vary significantly between individuals, likely due to genetic determinants. In humans, allergy development is initiated by antigen-presenting cells via class II human leukocyte antigen (HLA-II). The HLA-II gene is highly polymorphic, and its allelic variance is thought to influence the susceptibility of individuals to a particular allergen. However, whether antigen presentation by different HLA-II variants contributes to symptom variation is not clear. We hypothesized that HLA-II allelic variance affects symptom phenotypes, including immediate physical reactions and delayed behavioral changes, in individuals with food hypersensitivity. To test our hypothesis, male and female mice of three transgenic …
Simtutor Module: Antibodies, Heather Gilbert
Simtutor Module: Antibodies, Heather Gilbert
Teaching, Learning, and Scholarship (TLAS) Academy Projects
Antibody simulation module developed for use in MLS 226: Introduction to Clinical Immunology and Molecular Diagnostics. The module was designed to reinforce lecture material and was developed utilizing SimTutor software.
Learning outcomes:
- Define antibody.
- Differentiate between innate and adaptive immunity.
- Differentiate between cellular and humoral immunity.
- Describe the four pathways of antibody acquisition.
- Identify the three functions of an antibody.
- Identify and describe the structure of an antibody including the following components and regions: light chain, heavy chain, constant region, variable region, Fc Fragment, Fab Fragment, and hinge region.
- List the antibody classes and their key features.
- Differentiate between the …
Leukocytes As Mediators Of Gut-Brain Communication, Dilini Ekanayake
Leukocytes As Mediators Of Gut-Brain Communication, Dilini Ekanayake
Biomedical Sciences Posters and Presentations
Food allergies
▪ Reactions range from mild/delayed to severe/rapid. ▪ People with mild allergic reactions have increased re-exposure risks. ▪ Cow’s milk allergy tends to manifest with milder allergic reactions.
Cow’s milk allergy (CMA)
▪ CMA has been associated with behavioral and neurological disorders. ▪ How allergic inflammatory signals from the gut reach the brain is unclear
Investigating The Potential Role Of Akkermansia Muciniphila Supplementation In Neuroinflammation: A Progress Report, Geetika Verma
Investigating The Potential Role Of Akkermansia Muciniphila Supplementation In Neuroinflammation: A Progress Report, Geetika Verma
Biomedical Sciences Posters and Presentations
Gut bacteria are important for proper development and function of the host immune system (1). Recent studies have provided association of altered microbiome in both food allergy and neuropsychiatric disorders (2, 3) indicating potential role of microbiome in regulating Gut-Brain-Axis. Akkermansia muciniphila belongs to the phylum Verrucomicrobia, is a commensal mucin degrading bacterial species (4). A. muciniphila protects the gut barrier by facilitating host mucus production. Since patients with food allergy have increased gut permeability, protection of intestinal barrier by increased mucus production may be beneficial in preventing allergen and pathogen infiltrations, hence, minimizing inflammation. A. muciniphila is well known …
Cortical Demyelination And Depression-Like Behavior Are Associated With Histaminergic Dysregulation In A Mouse Model Of Peripheral Inflammation, Danielle Germundson-Hermanson
Cortical Demyelination And Depression-Like Behavior Are Associated With Histaminergic Dysregulation In A Mouse Model Of Peripheral Inflammation, Danielle Germundson-Hermanson
Biomedical Sciences Posters and Presentations
▪ Demyelinating diseases of the central nervous system are increasing in prevalence world-wide1,2 and manifest as motor, behavioral, and/or cognitive defects3 . The etiology and pathophysiology of demyelinating diseases remain unclear.
▪ We have previously observed cortical demyelination in our mouse model of non-anaphylactic cow’s milk allergy4 . The demyelination was associated with depression-like behaviors and region-specific increases in brain histamine and H3 receptor (H3R) levels4,5 .
❖ The brain’s central histaminergic system is tightly controlled and regulates many behaviors. Additionally, signaling through H3R plays a crucial role in oligodendrocyte differentiation, and thus, demyelination and remyelination6 .
▪ We hypothesized …
Repeated Allergen Exposure Induces Histaminergic Dysregulation And Depression-Like Behaviors In A Non-Anaphylactic Food Allergy Mouse Model, Danielle Germundson-Hermanson
Repeated Allergen Exposure Induces Histaminergic Dysregulation And Depression-Like Behaviors In A Non-Anaphylactic Food Allergy Mouse Model, Danielle Germundson-Hermanson
Biomedical Sciences Posters and Presentations
▪ Food allergy is often comorbid with neuropsychiatric disorders such as anxiety1,2, depression1,2, OCD3, ADD/ADHD4, and autism5. ➢ The mechanism of how food allergy may impact brain function and behavior in certain allergic individuals has yet to be elucidated.
▪ Mast cells (MCs) are not only the effector cell of the hypersensitivity response but are also important in maintaining brain homeostasis6. ➢ MCs have been implicated in neuropsychiatric and neurodegenerative disorders such as multiple sclerosis7,8.
▪ We have previously found in a mouse model of cow’s milk allergy (CMA) that intracranial MC numbers9 and histamine 3 receptor (H3R)10 increased after …
Continuous Consumption Of Whey Protein Affects Motor And Cognitive Functions In A Mouse Model Of Cow’S Milk Hypersensitivity, Geetika Verma
Continuous Consumption Of Whey Protein Affects Motor And Cognitive Functions In A Mouse Model Of Cow’S Milk Hypersensitivity, Geetika Verma
Biomedical Sciences Posters and Presentations
Hypersensitization to a food allergen can occur in the absence of immediate allergic reactions, a condition referred to as asymptomatic sensitization. Using mice asymptomatically sensitized to a bovine whey allergen, β-lactoglobulin (BLG, Bos d 5), we have previously shown that chronic exposure to the allergen results in behavior changes with increased neuroinflammation and cortical demyelination. However, whether allergen avoidance can resolve these pathologies is unknown. Thus, we aimed to compare immunological and behavioral changes in sham- or BLG-sensitized mice with or without the removal of dietary whey after the mice had consumed either a control diet (CTL) or a whey-protein-containing …
Continued Food-Allergen Consumption Exacerbates Beta-Amyloid Accumulation In Allergen-Sensitized Appnl-G-Fmice., Afrina Brishti
Continued Food-Allergen Consumption Exacerbates Beta-Amyloid Accumulation In Allergen-Sensitized Appnl-G-Fmice., Afrina Brishti
Biomedical Sciences Posters and Presentations
Alzheimer’s disease (AD) is the most common neurodegenerative disease, with β-amyloid(Aβ) plaque deposition being one of the hallmark pathologies. However, the etiology of AD remains elusive. While chronic inflammation from recurrent infections or injury seems to contribute to AD development, it is unclear whether atopic diseases, such as allergies, are associated with AD. We previously reported that continuous consumption of a whey protein(WP) -containing diet led to lasting neuro inflammation in C57BL/6J mice that were sensitized but tolerant to a bovine milk allergen, β-lactoglobulin (BLG;Bosd5). Thus, we hypothesized that the persisting neuroinflammation due to repeated allergen consumption would exacerbate AD …
Histamine H3 Receptor Antagonism Mitigates Food-Hypersensitivity-Associated Depressive Behavior And Neuropathology In A Mouse Model Of Cow’S Milk Allergy, Danielle Germundson-Hermanson
Histamine H3 Receptor Antagonism Mitigates Food-Hypersensitivity-Associated Depressive Behavior And Neuropathology In A Mouse Model Of Cow’S Milk Allergy, Danielle Germundson-Hermanson
Biomedical Sciences Posters and Presentations
Background Histamine is best known for causing allergy symptoms, but it also regulates blood-brain barrier permeability1 and oligodendrocyte differentiation2. Thus, brain histamine levels are tightly controlled.
Previous Findings We demonstrated that histamine and histamine H3 receptor (H3R) levels were elevated in a mouse model of cow’s milk allergy (CMA) in association with intracranial mast cell activation, depression-like behaviors, and cortical demyelination3,4.
Gaps in Knowledge In humans, food allergies are often associated with neuropsychiatric disorders5-7, but the involvement of allergy induced histamine in triggering behavioral changes is unclear.
Hypothesis Repeated allergen consumption can lead to central histaminergic dysfunction through H3R, ultimately …
Dietary Whey Protein Increases Brain Leukocytes In Mice Regardless Of Their Hypersensitivity Status, Dilini Ekanayake
Dietary Whey Protein Increases Brain Leukocytes In Mice Regardless Of Their Hypersensitivity Status, Dilini Ekanayake
Biomedical Sciences Posters and Presentations
Cow’s milk allergy (CMA) often manifests as milder reactions and may be linked to neurological problems. Previously, we demonstrated that C57BL/6J mice sensitized to a bovine whey allergen, β-lactoglobulin (BLG, Bos d 5), moderately increased BLG-specific IgE levels and exhibited behavioral changes without severe allergic reactions. When these non-anaphylactic CMA mice were placed on a whey-protein (WP)-containing diet for 2 weeks to simulate continuous dairy consumption, we found neuropathology indicative of neuroinflammation and cortical demyelination. Since immune cells migrate to the central nervous system (CNS) and promote neuroinflammation in demyelinating conditions such as multiple sclerosis, we hypothesized that the number …
Continuous Consumption Of Whey Protein Maintains Neuroinflammation In A Mouse Model Of Asymptomatic Cow’S Milk Allergy, Geetika Verma
Continuous Consumption Of Whey Protein Maintains Neuroinflammation In A Mouse Model Of Asymptomatic Cow’S Milk Allergy, Geetika Verma
Biomedical Sciences Posters and Presentations
Using a mouse model of cow’s milk allergy (CMA), we previously showed that C57BL/6J mice sensitized to a bovine whey allergen, β-lactoglobulin (BLG), exhibited anxiety/depression-like behavior and neuroinflammation in the absence of overt anaphylaxis upon allergen exposure
Milk Allergen Increases Intestinal Immune Cells In Association With Neuroinflammation And Behavioral Changes, Dilini Ekanayake
Milk Allergen Increases Intestinal Immune Cells In Association With Neuroinflammation And Behavioral Changes, Dilini Ekanayake
Biomedical Sciences Posters and Presentations
Cow’s milk allergy (CMA)
CMA h as been associated with neurological disorders.
How allergic inflammatory signals from the gut reach the brain is unclear.
Allergen Specific Immunotherapy And The Effect On Allergy Induced Asthma, Cord Tanner Landrum
Allergen Specific Immunotherapy And The Effect On Allergy Induced Asthma, Cord Tanner Landrum
Physician Assistant Scholarly Project Papers
Allergies are a major public health problem affecting over 300 million people worldwide (Asamoah et. al). An allergic response can occur when a harmless substance, such as tree pollen, is inhaled into the body and mistakenly identified as a harmful substance. Antibodies then bind to the allergen causing a release of chemicals that leads to symptoms such as nasal congestion, runny nose, itchy eyes or skin reactions. For some people, this reaction can also affect the lungs and airways leading to asthma symptoms.
This review analyzes articles from PubMed, Cochran, Clinical Key and references found from UpToDate articles to assess …
Introduction Of Peanuts To The Pediatric Patient, Leslee Graff
Introduction Of Peanuts To The Pediatric Patient, Leslee Graff
Physician Assistant Scholarly Project Posters
Peanut allergies have been diagnosed exponentially over the last ten-years and are more severe now than ever before. (DuToit et al. 2106) Some of the latest statistics are citing more than double the amount of peanut allergies diagnosed in the last few years. Guidelines set forth in 2010, by the American Academy of Allergy, Asthma, and Immunology stated that peanut, milk, soy, wheat, egg, and other tree nuts were to be avoided until three years of age, unless these patients have other chronic illnesses such as asthma or other atopic conditions. If so, the patient is to abstain from these …
Does The Hygiene Hypothesis Contribute To Autoimmune And Allergic Disease In Children With Focus On Type 1 Diabetes Mellitus And Asthma, Chad Briley
Physician Assistant Scholarly Project Posters
The Hygiene Hypothesis first introduced in 1989 by an epidemiologist, Dr Strachan, as he observed an increased prevalence of allergic diseases in society. Further clinical studies and research have included autoimmunity and inflammatory disease under the umbrella of the Hygiene Hypothesis. Article searches were done in the electronic medical database, Pubmed, for articles related to the Hygiene Hypothesis and autoimmunity related to Type 1 Diabetes Mellitus (T1DM) and hygiene hypothesis and childhood asthma. Articles written in the last five years were utilized for the project. The subject group is male and female children ages birth to 18 years old with …
Sublingual Immunotherapy: Is It A Viable Option To Subcutaneous Immunotherapy When Treating Allergic Rhinitis?, David Ryan Kesterson
Sublingual Immunotherapy: Is It A Viable Option To Subcutaneous Immunotherapy When Treating Allergic Rhinitis?, David Ryan Kesterson
Physician Assistant Scholarly Project Posters
• Background: Allergic rhinitis (AR) is a very common debilitating disease that can be detrimental to one’s lifestyle, work, and home life. Immunotherapy is the only treatment option that has disease modifying capabilities with a proper therapeutic regimen. Subcutaneous immunotherapy (SCIT) was developed over 100 years ago and has been the gold standard in immunotherapy for many years in the United States. During these years many adverse reactions have been reported with SCIT along with non-adherence to total treatment. The introduction of sublingual immunotherapy (SLIT) over 20 years ago gave providers a promising alternative to SCIT.
• Method: A review …
Comparing Scit With Slit: For Treatment Of Ige Mediated Grass-Pollen Induced Rhinoconjunctivitis, Cory Heiser
Comparing Scit With Slit: For Treatment Of Ige Mediated Grass-Pollen Induced Rhinoconjunctivitis, Cory Heiser
Physician Assistant Scholarly Project Posters
• The peer review literature compared the effectiveness of treating allergic rhinitis (AR) with subcutaneous immunotherapy (SCIT) versus sublingual immunotherapy (SLIT) for allergen-induced rhinoconjunctivitis.
• The findings showed that there is no clear evidence of effectiveness between SCIT and SLIT for reducing AR symptoms. SLIT has been associated with cost savings with comparison to SCIT.
• The findings indicated that SLIT may be the preferred route for immunotherapy because of its convenience, comparable efficacy, safety and cost.
Oral Immunotherapy In Ige-Mediated Cow’S Milk Protein Allergy, Jody Bauer
Oral Immunotherapy In Ige-Mediated Cow’S Milk Protein Allergy, Jody Bauer
Physician Assistant Scholarly Project Posters
Cow’s milk allergy prevalence in children has been increasing significantly over the last several decades. The diagnosis of a cow’s milk allergy (CMA) can occur through several methods such as signs and symptoms, double blind oral food challenge, skin prick testing, IgE and IgG4 serum levels. Currently, there are no treatments beyond strict allergen avoidance. The patient’s quality of life is impacted with the fear of inadvertent exposure resulting in allergic reactions that may be life threatening. Recent studies have explored desensitization to CM using oral immunotherapy (OIT), subcutaneous immunotherapy, and sublingual immunotherapy (SLIT). Research has found that desensitization will …