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Full-Text Articles in Medicine and Health Sciences
Molecular Manipulation Of Keratin 8/18 Intermediate Filaments: Modulators Of Fas-Mediated Death Signaling In Human Ovarian Granulosa Tumor Cells., Sarah K. Trisdale, Nicolette M. Schwab, Xiaoying Hou, John S. Davis, David H. Townson
Molecular Manipulation Of Keratin 8/18 Intermediate Filaments: Modulators Of Fas-Mediated Death Signaling In Human Ovarian Granulosa Tumor Cells., Sarah K. Trisdale, Nicolette M. Schwab, Xiaoying Hou, John S. Davis, David H. Townson
Journal Articles: Obstetrics & Gynecology
BACKGROUND: Granulosa cell tumors (GCT) are a rare ovarian neoplasm but prognosis is poor following recurrence. Keratin intermediate filaments expressed in these tumors are a diagnostic marker, yet paradoxically, may also constitute a target for therapeutic intervention. In the current study, we evaluated keratin 8/18 (K8/18) filament expression as a mechanism of resistance to apoptosis in GCT, specifically focusing on regulation of the cell surface death receptor, Fas (FAS).
METHODS: The GCT cell line, KGN, was transiently transfected with siRNA to KRT8 and KRT18 to reduce K8/18 filament expression. Expression of K8/18, FAS, and apoptotic proteins (PARP, cleaved PARP) were …