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Full-Text Articles in Medicine and Health Sciences
Comparison Of Two Automated Immunohistochemical Procedures For The Diagnosis Of Scrapie In Domestic Sheep And Chronic Wasting Disease In North American White-Tailed Deer (Odocoileus Virginianus) And Mule Deer (Odocoileus Hemionus), T. V. Baszler, M. Kiupel, E. S. Williams, B. V. Thomsen, T. Gidlewski, D. L. Montgomery, Katherine I. O'Rourke, S. M. Hall
Comparison Of Two Automated Immunohistochemical Procedures For The Diagnosis Of Scrapie In Domestic Sheep And Chronic Wasting Disease In North American White-Tailed Deer (Odocoileus Virginianus) And Mule Deer (Odocoileus Hemionus), T. V. Baszler, M. Kiupel, E. S. Williams, B. V. Thomsen, T. Gidlewski, D. L. Montgomery, Katherine I. O'Rourke, S. M. Hall
Other Publications in Zoonotics and Wildlife Disease
Two commercially available automated immunohistochemistry platforms, Ventana NexES and DakoCytomation Autostainer Universal Staining System, were compared for diagnosing sheep scrapie and cervid chronic wasting disease. Both automated platforms used the same antiprion protein monoclonal primary antibodies, but different platform-specific linker and amplification reagents and procedures. Duplicate sections of brainstem (at the level of the obex) and lymphoid tissue (retropharyngeal lymph node or tonsil) from the same tissue block were immunostained for the comparison. Examination of 1,020 tissues from 796 sheep revealed 100% concordance of results between the Ventana NexES and DakoCytomation platforms for diagnosing sheep scrapie from lymphoid tissue (103/103 …
Preliminary Observations Of Genetic Susceptibility Of Elk (Cervus Elaphus Nelsoni) To Chronic Wasting Disease By Experimental Oral Inoculation, Amir N. Hamir, Thomas Gidlewski, Terry R. Spraker, Janice M. Miller, Lynn Creekmore, Michelle Crocheck, Thomas Cline, Katherine I. O'Rourke
Preliminary Observations Of Genetic Susceptibility Of Elk (Cervus Elaphus Nelsoni) To Chronic Wasting Disease By Experimental Oral Inoculation, Amir N. Hamir, Thomas Gidlewski, Terry R. Spraker, Janice M. Miller, Lynn Creekmore, Michelle Crocheck, Thomas Cline, Katherine I. O'Rourke
Other Publications in Zoonotics and Wildlife Disease
To compare the genetic susceptibility of elk (Cervus elaphus nelsoni) with various alleles of the PRNP gene, which encodes the normal cellular prion protein, to chronic wasting disease (CWD), eight 8-monthold elk calves of 3 genotypes (2 132MM, 2 132LM, and 4 132LL) were orally dosed with CWD-infected brain material from elk. During postinoculation (PI) month 23, both 132MM elk had lost appetite, developed clinical signs of weight loss and central nervous system (CNS) dysfunction, and were euthanized. Two other elk (both 132LM) developed similar clinical signs of disease and were euthanized during PI month 40. All 4 …