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Full-Text Articles in Medicine and Health Sciences
Harvesting Murine Alveolar Macrophages And Evaluating Cellular Activation Induced By Polyanhydride Nanoparticles, Ana V. Chavez-Santoscoy, Lucas M. Huntlimer, Amanda E. Ramer-Tait, Michael J. Wannemeuhler, Balaji Narasimhan
Harvesting Murine Alveolar Macrophages And Evaluating Cellular Activation Induced By Polyanhydride Nanoparticles, Ana V. Chavez-Santoscoy, Lucas M. Huntlimer, Amanda E. Ramer-Tait, Michael J. Wannemeuhler, Balaji Narasimhan
Amanda Ramer-Tait Publications
Biodegradable nanoparticles have emerged as a versatile platform for the design and implementation of new intranasal vaccines against respiratory infectious diseases. Specifically, polyanhydride nanoparticles composed of the aliphatic sebacic acid (SA), the aromatic 1,6-bis(p-carboxyphenoxy)hexane (CPH), or the amphiphilic 1,8-bis(p-carboxyphenoxy)-3,6-dioxaoctane (CPTEG) display unique bulk and surface erosion kinetics1,2 and can be exploited to slowly release functional biomolecules (e.g., protein antigens, immunoglobulins, etc.) in vivo3,4,5. These nanoparticles also possess intrinsic adjuvant activity, making them an excellent choice for a vaccine delivery platform6,7,8.
In order to elucidate the mechanisms governing the activation of innate …
Chemistry-Dependent Adsorption Of Serum Proteins Onto Polyanhydride Microparticles Differentially Influences Dendritic Cell Uptake And Activation, Brenda R. Carrillo-Conde, Amanda E. Ramer-Tait, Michael J. Wannemeuhler, Balaji Narasimhan
Chemistry-Dependent Adsorption Of Serum Proteins Onto Polyanhydride Microparticles Differentially Influences Dendritic Cell Uptake And Activation, Brenda R. Carrillo-Conde, Amanda E. Ramer-Tait, Michael J. Wannemeuhler, Balaji Narasimhan
Amanda Ramer-Tait Publications
The delivery of antigen-loaded microparticles to dendritic cells (DCs) may benefit from surface optimization of the microparticles themselves, thereby exploiting the material properties and introducing signals that mimic pathogens. Following in vivo administration microparticle surface characteristics are likely to be significantly modified as proteins are quickly adsorbed onto their surface. In this work we describe the chemistry-dependent serum protein adsorption patterns on polyanhydride particles and the implications for their molecular interactions with DCs. The enhanced expression of MHC II and CD40 on DCs after incubation with amphiphilic polyanhydride particles, and the increased secretion of IL-6, TNF-α, and IL-12p40 by hydrophobic …