Medicine and Health Sciences Commons^™

Disparate Degrees Of Hypervariable Loop Flexibility Control T Cell Receptor Cross-Reactivity, Specificity, And Binding Mechanism, Daniel R. Scott, Oleg Y. Borbulevych, Kurt H. Piepenbrink, Steven A. Corcelli, Brian M. Baker

Food for Health: Publications

αβ T cell receptors recognize multiple antigenic peptides bound and presented by major histocompatibility complex molecules. TCR cross-reactivity has been attributed in part to flexibility of the complementarity-determining region loops, yet there have been limited direct studies of loop dynamics to determine the extent of its role. Here we studied the flexibility of the binding loops of the αβ TCR A6 utilizing crystallographic, spectroscopic, and computational methods. A significant role for flexibility in binding and cross-reactivity was indicated only for the CDR3α and CDR3β hypervariable loops. Examination of the energy landscapes of these two loops indicated that CDR3β possesses a …

Cellular Poly(C) Binding Proteins 1 And 2 Interact With Porcine Reproductive And Respiratory Syndrome Virus Nonstructural Protein 1Β And Support Viral Replication, Lalit Beura, Phat X. Dinh, Fernando A. Osorio, Asit K. Pattnaik

School of Veterinary and Biomedical Sciences: Faculty Publications

Porcine reproductive and respiratory syndrome virus (PRRSV) infection of swine results in substantial economic losses to the swine industry worldwide. Identification of cellular factors involved in PRRSV life cycle not only will enable a better understanding of virus biology but also has the potential for the development of antiviral therapeutics. The PRRSV nonstructural protein 1 (nsp1) has been shown to be involved in at least two important functions in the infected hosts: (i) mediation of viral subgenomic (sg) mRNA transcription and (ii) suppression of the host’s innate immune response mechanisms. To further our understanding of the role of the viral …

Examining The Effect Of Medical Risk, Parental Stress, And Self-Efficacy On Parent Behaviors And The Home Environment Of Premature Children, Kathryn Woods

College of Education and Human Sciences: Dissertations, Theses, and Student Research

The purpose of this study was to examine the relationship between medical risk and parenting stress and the extent to which parental self-efficacy moderates the relationship between medical risk, parenting stress, specific parenting behaviors (i.e., parental responsivity, acceptance of child, parental involvement) and the home environment (i.e., organization of environment, learning materials, variety in experience, and IT-HOME total score) of premature children. Participants included 72 parent-child dyads with premature children between the ages of 7 and 35 months corrected age. Measures included parent reports of medical risk, stress, self-efficacy, and the IT-HOME. Results show that medical risk was not significantly …

Guinea Pigging In Philadelphia, Roberto Abadie

Department of Sociology: Faculty Publications

On June 16, 2001, the national press first reported the death of Ellen Roche, a healthy 24-year-old who volunteered for an asthma study at Johns Hopkins University. The story revealed that a few days into the trial she felt very sick, was discharged, and sent home. Within some hours she checked into the emergency room at a local hospital and fell into a coma. Ellen remained in this state until her death a month later. She had received $375 for participating in seven to nine sessions as an outpatient in the clinical drug study that resulted in her death.

This …

Constructive Developmental Theory: An Alternative Approach To Leadership, Anne Marie B. Hunter, Nancy M. Lewis, Paula K. Ritter-Gooder

Department of Nutrition and Health Sciences: Faculty Publications

As early as 1954, the critical value of leadership in dietetics was described by Rourke, who wrote, “When the day comes that your executive abilities equal your scientific knowledge, your profession (dietetics) will be secure. Until that day, you will be faced with a constant and unwelcome challenge” (1). Career and leadership skills development have been identified as an area for further attention by American Dietetic Association (ADA) practitioners and student members (2). Creation of the ADA Leadership Institute in 2003 represented an awareness of the need to invest in developing dietetic leaders (3). Proficient leaders are critical in keeping …

Empathy-Based Conservation: An Interdisciplinary Approach To Conservation Policy And Decision-Making, Kaitlyn Delashmutt

Department of Environmental Studies: Undergraduate Student Theses

In the late 20^th century, neuroscientists in Italy discovered a neuron in the brain capable of mentally mimicking the emotions derived from the actions of others (Rizzolatti and Craighero, 2004). It is the process that makes your elbow ache when someone else knocks their elbow on the counter or the uncontrollable smile that creeps up when someone smiles at you. No questions asked, people intuitively sense what others are feeling. The old school of thought was that humans deduced through logic and reason the actions of others and interpreted the emotions through a rational process (Carew et al, 2008). …

Species D Adenoviruses As Oncolytics Against B-Cell Cancers, Christopher Y. Chen, Julien S. Senac, Eric A. Weaver, Shannon M. May, Diane F. Jelinek, Philip Greipp, Thomas Witzig, Michael A. Barry

Nebraska Center for Virology: Faculty Publications

Purpose: Oncolytic viruses are self-amplifying anticancer agents that make use of the natural ability of viruses to kill cells. Adenovirus serotype 5 (Ad5) has been extensively tested against solid cancers, but less so against B-cell cancers because these cells do not generally express the coxsackie and adenoviral receptor (CAR). To determine whether other adenoviruses might have better potency, we "mined" the adenovirus virome of 55 serotypes for viruses that could kill B-cell cancers.

Experimental Design: Fifteen adenoviruses selected to represent Ad species B, C, D, E, and F were tested in vitro against cell lines and primary patient B-cell cancers …

Vocational Experiences Of Survivors Of Severe Tbi With Diverse Employment Patterns: An Explanatory Mixed Method Design, Erin J. Bush

College of Education and Human Sciences: Dissertations, Theses, and Student Research

Employment status is a salient outcome following traumatic brain injury (TBI). A return to productive activity relates in complex ways to quality of life, and loss of employment competence has potentially devastating effects on survivors. Currently, inadequate information exists about the distribution of occupations held by survivors, post-injury employment stability, and the frequency that survivors return to their pre-injury occupations. The research presented herein addressed these issues. This study consisted of two phases. Through Phase 1, the researcher gathered quantitative employment data regarding a pool of 283 survivors of severe TBI. She then conducted telephone interviews of family members of …

Surveillance Of Selected Diseases In Free-Ranging Elk (Cervus Elaphus Nelsoni) In Nebraska, 1995-2009, Michael A. Cover, Scott E. Hygnstrom, David W. Oates, Kit M. Hams, Kurt C. Vercauteren

Great Plains Research: A Journal of Natural and Social Sciences

Sera samples were collected from 21 free-ranging, captured female elk (Cervus elaphus nelsoni) in 1995- 96, and tissue and sera samples were collected from 415 hunter-harvested elk from 1995 to 2006 and tested for selected diseases. Titers for Anaplasma marginale were detected in 81 of 436 (19%) elk. Occurrence of antibodies to anaplasmosis increased from 4 to 40 elk from 2002 to 2006. Titers for bovine viral diarrhea virus (BVDV) were detected in 18 of 346 (5%) samples. Titers for Leptospira interrogans serovars were detected in 21 of 289 (7%) of samples from 1995 to 2004. Titers for …

Perceived Importance And Actual Intake Of Calcium And Vitamin D In Young Female Athletes, Miriam E. Zambrano

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

Calcium needs increase as a child grows. Actual intake often decreases as a child gets older, leaving his/her developing bones at risk for injury during childhood and increasing the risk of developing osteoporosis later in life. The purpose of this study was to investigate relationships between psychosocial factors influencing consumption of calcium and vitamin D-rich foods and actual intakes of these nutrients. We examined the dietary calcium and vitamin D intakes, determined serum vitamin D levels, and assessed nutrition knowledge and perception of a sample of 20 young girls participating in competitive cheerleading in Omaha, Nebraska. From this information we …

Antagonistic Effects Of Cellular Poly(C) Binding Proteins On Vesicular Stomatitis Virus Gene Expression, Phat X. Dinh, Lalit Beura, Debasis Panda, Anshuman Das, Asit K. Pattnaik

School of Veterinary and Biomedical Sciences: Faculty Publications

Immunoprecipitation and subsequent mass spectrometry analysis of the cellular proteins from cells expressing the vesicular stomatitis virus (VSV) P protein identified the poly(C) binding protein 2 (PCBP2) as one of the P protein-interacting proteins. To investigate the role of PCBP2 in the viral life cycle, we examined the effects of depletion or overexpression of this protein on VSV growth. Small interfering RNA-mediated silencing of PCBP2 promoted VSV replication. Conversely, overexpression of PCBP2 in transfected cells suppressed VSV growth. Further studies revealed that PCBP2 negatively regulates overall viral mRNA accumulation and subsequent genome replication. Coimmunoprecipitation and immunofluorescence microscopic studies showed that …

Acute Fatty Liver Of Pregnancy: An Update On Mechanisms, Sathish Kumar Natarajan, Kavitha R. Thangaraj, Ashish Goel, C. E. Eapen, K. A. Balasubramanian, Anup Ramachandran

School of Veterinary and Biomedical Sciences: Faculty Publications

Acute fatty liver of pregnancy (AFLP), characterized by hepatic microvesicular steatosis, is a sudden catastrophic illness occurring almost exclusively in the third trimester of pregnancy. Defective fatty acid oxidation in the fetus has been shown to be associated with this disease. Since the placenta has the same genetic makeup as the fetus and as AFLP patients generally recover following delivery, we hypothesized that the placenta might be involved in pathogenesis of this disease. In an animal model of hepatic microvesicular steatosis (using sodium valproate), we found that microvesicular steatosis results in mitochondrial structural alterations and oxidative stress in subcellular organelles …

Time Course Of Prepulse Inhibition Disruption Induced By Dopamine Agonists And Nmda Antagonists: Effects Of Drug Administration Regimen, Ming Li, Wei He, Jing Chen

Department of Psychology: Faculty Publications

Prepulse inhibition (PPI) of acoustic startle response is impaired in patients with schizophrenia and in animals acutely treated with dopamine agonists and NMDA antagonists. In this study, we investigated the time course of PPI disruption induced by repeated amphetamine, quinpirole, phencyclidine (PCP), and dizocilpine (MK-801) treatment. We focused on how PPI disruption development was influenced by drug administration regimens, comparing a constant versus an escalating dosing regimen. Male Sprague–Dawley rats were repeatedly treated with amphetamine (1.25–5.0 mg/kg, or constant 5.0 mg/kg, sc), PCP (0.50–2.0 mg/kg, or constant 0.5, 1.0 or 2.0 mg/kg, sc), quinpirole (0.03–0.12 mg/kg, or constant 0.12 mg/kg, …

Interpersonal Aggression Perpetration: Static And Emotion Regulation Risk Factors, Jill Panuzio

Department of Psychology: Dissertations, Theses, and Student Research

Intimate partner aggression (IPA) is a serious public health problem for both men and women in the United States. With aspirations of alleviating the significant negative effects of IPA, a substantial body of literature has been devoted to uncovering risk factors for IPA perpetration. Much of this research has focused on static, or relatively stable, factors that may influence IPA, such as life stress, distress tolerance, rumination, and jealousy. However, considering situational variables that influence individuals more proximally to aggressive acts, in conjunction with these static factors, may provide more precise prediction of partner aggression. Current theoretical and empirical work …

Glutaredoxin 2 Prevents H2o2-Induced Cell Apoptosis By Protecting Complex I Activity In The Mitochondria, Hongli Wu, Kuiyi Xing, Marjorie F. Lou

School of Veterinary and Biomedical Sciences: Faculty Publications

Glutaredoxin 2 (Grx2) belongs to the oxidoreductase family and is an isozyme of glutaredoxin 1 (Grx1) present in the mitochondria, however its function is not well understood. The purpose of this study is to evaluate the potential anti-apoptotic function of Grx2 by examining its ability to protect complex I in the mitochondrial electron transport system using human lens epithelial cells as a model. We found that cells treated with 200 μM hydrogen peroxide (H2O2) for 24 h exhibited decreased viability and became apoptotic with corresponding Bax up-regulation, Bcl-2 down-regulation, caspase 3 activation and mitochondrial cytochrome c leakage. Grx2 over-expression (OE) …

The Cumulative Impact Of Sexual Revictimization On Emotion Regulation Difficulties: An Examination Of Female Inmates, Kate Walsh, David K. Dilillo, Mario J. Scalora

Department of Psychology: Faculty Publications

The present study examined associations between child sexual abuse (CSA), adult sexual victimization, and emotion regulation difficulties in a sample of 168 incarcerated women. Approximately 50% of the participants reported CSA, 54% reported adult sexual victimization, and 38% reported sexual revictimization (i.e., CSA and adult victimization). Revictimized women reported significantly greater difficulties with several facets of emotion regulation when compared to singly victimized and nonvictimized women. Interestingly, singly victimized women did not demonstrate greater emotion regulation deficits when compared to nonvictims. Findings suggest that the negative impact of victimization experiences on adult emotion regulation abilities may be cumulative. Furthermore, they …

Initial Mlu Predicts The Relative Efficacy Of Two Grammatical Treatments In Preschoolers With Specific Language Impairments, Paul J. Yoder, Dennis L. Molfese, Elizabeth Gardner

Center for Brain, Biology, and Behavior: Faculty and Staff Publications

Purpose—We sought to confirm predictions based on past findings that pre-treatment mean length of utterance (MLU) would predict which of two grammatical treatments would best facilitate generalized and maintained grammatical development in preschoolers with specific language impairment (SLI).

Method—The participants were 57 preschoolers with specific language impairment (SLI). A randomized group experiment was used. The two grammatical treatments were Broad Target Recasts (BTR) and Milieu Language Teaching (MLT). MLU was assessed at Time 1 in two conversational language samples. Growth rate of productive grammar was quantified using growth curve modeling on the Index of Productive Syntax (IPSyn) from …

The Development Of Future Orientation: Underpinnings And Related Constructs, Sarah J. Beal

Department of Psychology: Dissertations, Theses, and Student Research

Future orientation has been conceptualized in a variety of ways across literatures in psychology, sociology, education, and vocation. The lack of a shared definition and measurement across theoretical perspectives has resulted in a challenge in comparing findings across literatures and organizing results in a way that provides a coherent sense of how future orientation impacts later outcomes. Trommsdorff (1979) provided a comprehensive definition of future orientation that included eight dimensions: extension, detail, domain, affect, motivation, control, sequence of events, and number of cognitions. Study 1 was designed to test this definition using measures from five prominent theories of future orientation …

Quantifying Articulatory Distinctiveness Of Vowels, Jun Wang, Jordan R. Green, Ashok Samal, David B. Marx

Department of Special Education and Communication Disorders: Faculty Publications

The articulatory distinctiveness among vowels has been frequently characterized descriptively based on tongue height and front-back position; however, very few empirical methods have been proposed to characterize vowels based on time-varying articulatory characteristics. Such information is not only needed to improve knowledge about the articulation of vowels but also to determine the contribution of articulatory imprecision to poor speech intelligibility. In this paper, a novel statistical shape analysis was used to derive a vowel space that depicted the quantified articulatory distinctiveness among vowels based on tongue and lip movements. The effectiveness of the approach was supported by vowel classification accuracy …

Identification Of Host Proteins Required For Vesicular Stomatitis Virus Infection, Debasis Panda

School of Veterinary and Biomedical Sciences: Dissertations, Theses, and Student Research

Viruses usurp host cell pathways for different stages of their infection. Understanding virus-host interaction will be invaluable to elucidate molecular mechanisms of virus infection and to identify drug targets. In order to identify such critical cellular genes in vesicular stomatitis virus (VSV, a model non-segmented negative strand RNA virus) infection, we developed a stable cell line constitutively expressing replication proteins of VSV. Attempts to establish a cell line replicating a sub-genomic replicon was not successful because of induction of interferon response by replication of viral genomic analog. Subsequently, we used siRNA technology and conducted a genome-wide siRNA screen in HeLa …

Effect Of The Infection With Porcine Reproductive And Respiratory Syndrome Virus On The Regulation Of Cytokines - Tumor Necrosis Factor-Alpha And Interleukin-10, Sakthivel Subramaniam

School of Veterinary and Biomedical Sciences: Dissertations, Theses, and Student Research

Porcine reproductive and respiratory syndrome virus (PRRSV) causes late-term abortion in sows and pneumonia in growing piglets. PRRSV evades the host immune response by several mechanisms, including the modulation of cytokine secretions in infected pigs, which is the subject of this dissertation. Particularly, PRRSV reduces the secretion of the pro-inflammatory cytokine, tumor necrosis factor-α (TNF-α) but increases the secretion of the anti-inflammatory cytokine interleukin-10 (IL-10). The latter effect, however, is PRRSV strain-specific. In this dissertation, we have examined mechanisms by which PRRSV regulates TNF-α and IL-10 expressions. The pathogenic strain FL12, derived from a PRRSV infectious clone, consistently suppressed TNF-α …

Role Of Porcine Reproductive And Respiratory Syndrome Virus Non-Structural Protein 1 In Viral Replication And Pathogenesis, Lalit Beura

School of Veterinary and Biomedical Sciences: Dissertations, Theses, and Student Research

Porcine reproductive and respiratory syndrome virus (PRRSV) is an infectious agent of significant concern to the global swine industry. PRRSV infection of pigs is initiated by a long viremia after which the virus enters in an extended persistent phase of 3-4 months that ultimately is resolved by the immune system. The delayed and weak host adaptive response is responsible for the protracted convalescence period. An initial sub-optimal innate immune response is postulated to be the reason behind such meager adaptive immune response. A major focus of the studies undertaken in this dissertation was to identify the viral non-structural proteins (nsps) …

Effects Of Single Nucleotide Polymorphisms In The Human Holocarboxylase Synthetase Gene On Catalytic Activity, Shingo Esaki

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

Holocarboxylase synthetase (HCS) catalyzes the covalent binding of biotin to carboxylases and histones in eukaryotic cells. Biotinylated carboxylases play essential roles in the metabolism of fatty acids, amino acids, and glucose; biotinylated histones play essential roles in gene regulation and genome stability. HCS null individuals are not viable whereas HCS deficiency is linked to developmental delays and phenotypes such as short life span and low stress resistance. Greater than 2,500 single nucleotide polymorphisms (SNPs) have been reported for HCS, but the biological importance of these polymorphisms is unknown. We hypothesized that some of these SNPs impair catalytic activity and that …

Comparison Of Adenoviruses As Oncolytics And Cancer Vaccines In An Immunocompetent B Cell Lymphoma Model, Eric A. Weaver, Christopher Y. Chen, Shannon M. May, Mary E. Barry, Michael A. Barry

Nebraska Center for Virology: Faculty Publications

We have screened human adenoviruses (Ads) for oncolytic activity against a variety of mouse and hamster cell lines and have found a number that are susceptible to a variety of Ad serotypes. A20 lymphoma is derived from BALB/c mice and is susceptible to infection and killing by a variety of human Ads. A20 is also a suitable cancer vaccine model, because these cells express a unique immunoglobulin variable region that can be targeted by vaccination. To compare Ads as cancer vaccines versus Ads as oncolytics, A20 tumors were initiated in immunocompetent BALB/c mice. Mice immunized with first-generation Ad5 expressing the …

Regulation Of Cytosolic Phospholipase A2 (Cpla2) And Its Association With Cell Proliferation In Human Lens Epithelial Cells, Yin Wang, Kui-Yi Xing, Marjorie F. Lou

School of Veterinary and Biomedical Sciences: Faculty Publications

PURPOSE. To investigate the molecular mechanism for cytosolic phospholipase A2 (cPLA2) regulation and its association to platelet-derived growth factor (PDGF)-induced cell proliferation.

METHODS. cPLA2 was examined using human lens epithelial (HLE) B3 cells. Reactive oxygen species (ROS) generation induced by PDGF was analyzed by luminescence assay. Cell proliferation was measured by cell counting and by BrdU assay. Human cPLA2 gene was cloned via RT-PCR followed by sitedirected mutagenesis to construct HLE B3 cells expressing either inactive cPLA2 enzyme with S228A mutation (S228A), or cPLA2 truncated at the calcium-binding C2 domain (C2D). Activity of cPLA2 was measured by arachidonic acid (AA) …

Visual Attention And Social Anxiety: Oculomotor Behavior When Threatened, Jacqueline S. Singh

Department of Psychology: Dissertations, Theses, and Student Research

A growing theoretical and research literature suggests that trait and state social anxiety can predict attentional patterns in the presence of emotional stimuli. The current study addressed some inconsistencies and gaps in the literature using eye tracking methodology. Participants with high and low trait social anxiety were randomly assigned to either give a speech or to watch a video of another individual delivering a speech (state social anxiety manipulation). Next, participants were asked to engage in a free view task in which pairs of emotional facial stimuli (angry-happy, angry-neutral, or happy-neutral) were presented for 3 s. Eye movements were monitored …

Exploratory Analyses Of A Developmental Conceptualization Of Insight And Treatment Outcomes Of Individuals With Serious Mental Illness In Psychiatric Rehabilitation, Ashley R. Wynne

Department of Psychology: Dissertations, Theses, and Student Research

The purpose of the present study was to further examine the relationship between adolescent psychiatric pathology and SMI by assessing the relationship between prior mental health services before the age of 18 and time of assessment on people’s insight into their illnesses. A secondary relationship between adolescent psychiatric pathology and functioning in a variety of domains before, during, and after treatment was assessed. Overall, there was an inconsistent pattern of results and partial support of hypotheses. The current study was a retrospective longitudinal study in which assessments were given to 308 participants in an inpatient psychiatric rehabilitation unit every 6 …

Why Confronting Sexism Works: Applying Persuasion Theories To Confronting Sexism, Amy Hillard

Department of Psychology: Dissertations, Theses, and Student Research

Speaking up about or confronting prejudice creates more positive attitudes, but the mechanism underlying confrontation’s prejudice reducing effect remains unclear. Based on an integration of the confronting prejudice and persuasion literatures, I expected that observing a confrontation (vs. no confrontation) reduces prejudice and discrimination; that elaborating on confrontation messages reduces prejudice and discrimination more than confrontation alone; and that elaborating on confrontation messages causes attitude change that lasts longer than confrontation alone. Participants were recruited to complete measures of sexism and feelings toward subtypes of women across three time points (i.e., pre-test, lab manipulation, and post-test). During the lab manipulation, …

When Knowing Just Isn't Enough: Examining The Role Of Moral Emotions In Health Decision Making Using The Theory Of Planned Behavior, Kate Duangdao

Department of Psychology: Dissertations, Theses, and Student Research

A proposed integrated Theory of Planned Behavior (TPB) model aimed to examine the role of moral emotions and two health outcomes: prosocial behaviors and smoking outcomes. Based on Tangney’s work with shame and guilt-proneness, it was expected that those more prone to guilt would engage in more prosocial behaviors and those more prone to shame would engage in more smoking behaviors. Prosocial behaviors were found to be negatively associated with smoking outcomes. However, results suggested that guilt and shame-proneness seem to function similarly in predicting behavioral outcomes. Components within the TPB were generally positively correlated with each health outcome, however …

Generation Of A Kupffer Cell-Evading Adenovirus For Systemic And Liver-Directed Gene Transfer, Reeti Khare, Shannon M. May, Francesco Vetrini, Eric A. Weaver, Donna Palmer, Amanda Rosewell, Nathan Grove, Philip Ng, Michael A. Barry

Nebraska Center for Virology: Faculty Publications

As much as 90% of an intravenously (i.v.) injected dose of adenovirus serotype 5 (Ad5) is absorbed and destroyed by liver Kupffer cells. Viruses that escape these cells can then transduce hepatocytes after binding factor X (FX). Given that interactions with FX and Kupffer cells are thought to occur on the Ad5 hexon protein, we replaced its exposed hypervariable regions (HVR) with those from Ad6. When tested in vivo in BALB/c mice and in hamsters, the Ad5/6 chimera mediated >10 times higher transduction in the liver. This effect was not due to changes in FX binding. Rather, Ad5/6 appeared to …