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Full-Text Articles in Medicine and Health Sciences
Effects Of Ethanol Extraction And Heat Treatment Of Soybean Flakes On Function And Morphology Of Pig Intestine, J. D. Hancock, E. R. Peo Jr., A. J. Lewis, Rodney A. Moxley
Effects Of Ethanol Extraction And Heat Treatment Of Soybean Flakes On Function And Morphology Of Pig Intestine, J. D. Hancock, E. R. Peo Jr., A. J. Lewis, Rodney A. Moxley
School of Veterinary and Biomedical Sciences: Faculty Publications
Digesta and tissue samples were collected from the intestinal tracts of 27 pigs to determine the relationship between intestinal morphology and the utilization of nutrients from soybean flakes. Soybean flake treatments were under-, intermediate- and overprocessed (Le., 5, 20 and 60 min of autoclaving) either without extraction or with heating before or after extraction with a 55% ethanol-water mixture. Final BW was greatest (P< .001) for pigs fed soybean flakes given 20 min of heat treatment. There was a trend (P < .09) for plasma lysine concentrations to be reduced when the unextracted soybean flakes were over-processed (60 min of heat). Differences in the flow rate of DM and N through the ileum and colon reflected differences in DM and N intake, rather than differences in intestinal function. The soybean flake treatments had no effect (P > .OS) on pH of the contents of the stomach, duodenum, ileum or colon. The ethanol extraction process increased (P < .001) N digestibility of the soybean flakes, especially when the soybean flakes were underprocessed (interaction, P < .02). Villus size (area, height and perimeter length) tended to be greater in pigs fed the soybean flakes heated after extraction and(or) exposed to the intermediate level of heat treatment. Indicators of villus shape (villus area/ villus height) and proliferative activity (crypt depth and villus heigh/crypt depth) were not affected by soybean flake treatment (P > .OS). Ethanol extraction and heat treatment affected the utilization of nutrients …
Replication And Amplification Of Defective Interfering Particle Rn As Of Vesicular Stomatitis Virus In Cells Expressing Viral Proteins From Vectors Containing Cloned Cdnas, Asit K. Pattnaik, Gail W. Wertz
Replication And Amplification Of Defective Interfering Particle Rn As Of Vesicular Stomatitis Virus In Cells Expressing Viral Proteins From Vectors Containing Cloned Cdnas, Asit K. Pattnaik, Gail W. Wertz
School of Veterinary and Biomedical Sciences: Faculty Publications
Replication and amplification of RNA genomes of defective interfering (DI) particles of vesicular stomatitis virus (VSV) depend on the expression of viral proteins and have untD now been attained only in ceUs coinfected with helper VSV. In the work described in this report, we used a recombinant vaccinia virus-T7 RNA polymerase expression system to synthesize individual VSV proteins in cells transfected with plasmid DNAs that contain cDNA copies of the VSV genes downstream of the T7 RNA polymerase promoter. In this way, we were able to examine the ability of VSV proteins, individually and in combination, to support DI particle …
Analysis Of The Transcriptional Promoter Which Regulates The Latency-Related Transcript Of Bovine Herpesvirus 1, Clinton J. Jones, G. Delhon, A. Bratanich, G. Kutish, D. Rock
Analysis Of The Transcriptional Promoter Which Regulates The Latency-Related Transcript Of Bovine Herpesvirus 1, Clinton J. Jones, G. Delhon, A. Bratanich, G. Kutish, D. Rock
School of Veterinary and Biomedical Sciences: Faculty Publications
As a transcriptional promoter in primary cultures of sensory ganglionic neurons, DNA sequences near the 5' terminus of the latency-related (LR) gene of bovine herpesvirus 1 were at least 10 times more efficient than the simian virus 40 early promoter-enhancer. In contrast, as a promoter in bovine, rodent, or monkey cells, the LR promoter was approximately six times less efficient than the simian virus 40 early promoter-enhancer. The LR promoter had strict orientation preferences in neurons and all other mammalian cell lines tested. Removal of a 146-base-pair XhoI fragment from the LR promoter resulted in stimulation of LR promoter …
Efficacy Of A Cell Extract From Actinobacillus (Haemophilus) Pleuropneumoniae Serotype 1 Against Disease In Swine, P. J. Fedorka-Cray, M.J. Huether, D.L. Stine, G. A. Anderson
Efficacy Of A Cell Extract From Actinobacillus (Haemophilus) Pleuropneumoniae Serotype 1 Against Disease In Swine, P. J. Fedorka-Cray, M.J. Huether, D.L. Stine, G. A. Anderson
School of Veterinary and Biomedical Sciences: Faculty Publications
We partially characterized a cell extract (CE) from Actinobacillus pleuropneumoniae serotype 1 and used the CE to test the efficacy of secreted proteins against disease. Secreted products from 4-h culture supernatants were precipitated with 20% polyethylene glycol. Analysis of the CE indicated the presence of protein, endotoxin, and carbohydrate. Hemolytic activity to bovine erythrocytes and cytotoxic activity to porcine mononuclear leukocytes was also demonstrated. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of the CE from a 4-h culture showed a major band at 110 kilodaltons (kDa), while a CE of a 26-h culture indicated the presence of a number of additional …