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Full-Text Articles in Medicine and Health Sciences
Alkaline Stabilization Of Manure Slurry Inactivates Porcine Epidemic Diarrhea Virus, Erin E. Stevens, Daniel N. Miller, Bethany A. Brittenham, Sarah J. Vitosh-Sillman, Bruce W. Brodersen, Virginia L. Jin, John D. Loy, Amy M. Schmidt
Alkaline Stabilization Of Manure Slurry Inactivates Porcine Epidemic Diarrhea Virus, Erin E. Stevens, Daniel N. Miller, Bethany A. Brittenham, Sarah J. Vitosh-Sillman, Bruce W. Brodersen, Virginia L. Jin, John D. Loy, Amy M. Schmidt
School of Veterinary and Biomedical Sciences: Faculty Publications
Hydrated lime manure treatment was evaluated to determine porcine epidemic diarrhea virus (PEDV) susceptibility to alkaline stabilization. At pH 10, PEDV decreased (quantitative polymerase chain reaction) and lost infectivity (swine bioassay). Although ammonium decreased above pH 9 (up to 25%), alkaline stabilization managed to control potential infection from manure sources.
In Situ Hybridization For The Detection And Localization Of Swine Chlamydia Trachomatis, C. Chae, D.-S. Cheon, D. Kwon, O. Kim, B. Kim, J. Suh, D. G. Rogers, K. D. E. Everett, A. A. Anderson
In Situ Hybridization For The Detection And Localization Of Swine Chlamydia Trachomatis, C. Chae, D.-S. Cheon, D. Kwon, O. Kim, B. Kim, J. Suh, D. G. Rogers, K. D. E. Everett, A. A. Anderson
School of Veterinary and Biomedical Sciences: Faculty Publications
Gnotobiotic piglets were inoculated intralaryngeally with swine Chlamydia trachomatis strain R33 or orally with swine C. trachmatis strain R27. Archived formalin-fixed, paraffin-embedded tissues from piglets euthanatized 4–7 days postinoculation were examined by in situ hybridization for C. trachomatis nucleic acid using a nonradioactive digoxigenin-labeled DNA probes that targeted specific ribosomal RNA or omp1 mRNA molecules of the swine C. trachomatis strains. Positive hybridization signals were detected in bronchial epithelial cells, bronchiolar epithelial cells, pneumocytes, alveolar and interstitial macrophages, and jejunal and ileal enterocytes. Chlamydia-infected cells had a strong signal that was confined to the intracytoplasmic inclusions. Positive hybridization signals were …
The Preparation Of Laboratory Specimens As An Aid To The Diagnosis Of Animal Diseases, L. Van Es, L. V. Skidmore
The Preparation Of Laboratory Specimens As An Aid To The Diagnosis Of Animal Diseases, L. Van Es, L. V. Skidmore
Nebraska Agricultural Experiment Station: Historical Circulars
Many problems in connection with animal diseases require for their solution the application of methods which can only be undertaken in properly equipped laboratories. This ordinarily involves the collection, packing, and shipping of samples of specimens. The manner in which this is done is a most important factor and to a very large extent is responsible either for the success or for the failure of the undertaking. The discussion of the subject to which the following pages are devoted is taken in hand in the hope that it may provide guidance to the veterinarians and others who may have occasion …
The Preparation Of Laboratory Specimens As An Aid To The Diagnosis Of Animal Diseases, L. Van Es, L. V. Skidmore
The Preparation Of Laboratory Specimens As An Aid To The Diagnosis Of Animal Diseases, L. Van Es, L. V. Skidmore
Nebraska Agricultural Experiment Station: Historical Circulars
Many problems in connection with animal diseases require for their solution the application of methods which can only be undertaken at properly equipped laboratories. This ordinarily involves the collection, packing, and shipping of samples of specimens. The manner in which this is done is a most important factor and to a very large extent is responsible either for the success or for the failure of the undertaking. The discussion of the subject to which the following pages are devoted is taken in hand in the hope that it may provide guidance to the veterinarians and others who may have occasion …