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Full-Text Articles in Medicine and Health Sciences
Cytokines: From Clinical Significance To Quantification, Chao Liu, Dewei Chu, Kourosh Kalantar-Zadeh, Jacob George, Howard A. Young, Guozhen Liu
Cytokines: From Clinical Significance To Quantification, Chao Liu, Dewei Chu, Kourosh Kalantar-Zadeh, Jacob George, Howard A. Young, Guozhen Liu
Public Health Resources
Cytokines are critical mediators that oversee and regulate immune and inflammatory responses via complex networks and serve as biomarkers for many diseases. Quantification of cytokines has significant value in both clinical medicine and biology as the levels provide insights into physiological and pathological processes and can be used to aid diagnosis and treatment. Cytokines and their clinical significance are introduced from the perspective of their pro- and anti-inflammatory effects. Factors affecting cytokines quantification in biological fluids, native levels in different body fluids, sample processing and storage conditions, sensitivity to freeze-thaw, and soluble cytokine receptors are discussed. In addition, recent advances …
Adaptive In Vivo Device For Theranostics Of Inflammation: Real-Time Monitoring Of Interferon-Γ And Aspirin, Chaomin Cao, Ronghua Jin, Hui Wei, Zhongning Liu, Shengnan Ni, Guo Jun Liu, Howard A. Young, Xin Chen, Guozhen Liu
Adaptive In Vivo Device For Theranostics Of Inflammation: Real-Time Monitoring Of Interferon-Γ And Aspirin, Chaomin Cao, Ronghua Jin, Hui Wei, Zhongning Liu, Shengnan Ni, Guo Jun Liu, Howard A. Young, Xin Chen, Guozhen Liu
Public Health Resources
Cytokines mediate and control immune and inflammatory responses. Complex interactions exist among cytokines, inflammation, and the innate and adaptive immune responses in maintaining homeostasis, health, and well-being. On-demand, local delivery of anti-inflammatory drugs to target tissues provides an approach for more effective drug dosing while reducing the adverse effects of systemic drug delivery. This work demonstrates a proof-of-concept theranostic approach for inflammation based on analyte-kissing induced signaling, whereby a drug (in this report, aspirin) can be released upon the detection of a target level of a proinflammatory cytokine (i.e., interferon-γ (IFN-γ)) in real time. The structure-switching aptamer-based biosensor described here …
Sera From Patients With Active Systemic Lupus Erythematosus Patients Enhance The Toll-Like Receptor 4 Response In Monocyte Subsets, Tiago Carvalheiro, Diane Gomes, Ligia A. Pinto, Luis Inês, Ana Lopes, Ana Henriques, Susana Pedreiro, António Martinho, Hélder Trindade, Howard A. Young, José António Pereira Da Silva, Artur Paiva
Sera From Patients With Active Systemic Lupus Erythematosus Patients Enhance The Toll-Like Receptor 4 Response In Monocyte Subsets, Tiago Carvalheiro, Diane Gomes, Ligia A. Pinto, Luis Inês, Ana Lopes, Ana Henriques, Susana Pedreiro, António Martinho, Hélder Trindade, Howard A. Young, José António Pereira Da Silva, Artur Paiva
Public Health Resources
Background: Systemic Lupus Erythematosus (SLE) is an auto-immune disease whose complex pathogenesis remains unraveled. Here we aim to explore the inflammatory ability of SLE patients' sera upon peripheral blood (PB) monocyte subsets and myeloid dendritic cells (mDCs) obtained from healthy donors. Methods: In this study we included 11 SLE patients with active disease (ASLE), 11 with inactive disease (ISLE) and 10 healthy controls (HC). PB from healthy donors was stimulated with patients' sera, toll-like receptor (TLR) 4 ligand - lipopolysaccharide or both. The intracellular production of TNF-α was evaluated in classical, non-classical monocytes and mDCs, using flow cytometry. TNF-α mRNA …