Open Access. Powered by Scholars. Published by Universities.®
University of Nebraska - Lincoln
Department of Chemical and Biomolecular Engineering: Faculty Publications
Articles 1 - 1 of 1
Full-Text Articles in Medicine and Health Sciences
Continuous Low-Intensity Ultrasound Attenuates Il-6 And Tnfα-Induced Catabolic Effects And Repairs Chondral Fissures In Bovine Osteochondral Explants, Neety Sahu, Hendrik J. Viljoen, Anuradha Subramanian
Continuous Low-Intensity Ultrasound Attenuates Il-6 And Tnfα-Induced Catabolic Effects And Repairs Chondral Fissures In Bovine Osteochondral Explants, Neety Sahu, Hendrik J. Viljoen, Anuradha Subramanian
Department of Chemical and Biomolecular Engineering: Faculty Publications
Background: Cartilage repair outcomes are compromised in a pro-inflammatory environment; therefore, the mitigation of pro-inflammatory responses is beneficial. Treatment with continuous low-intensity ultrasound (cLIUS) at the resonant frequency of 5 MHz is proposed for the repair of chondral fissures under pro-inflammatory conditions.
Methods: Bovine osteochondral explants, concentrically incised to create chondral fissures, were maintained under cLIUS (14 kPa (5 MHz, 2.5 Vpp), 20 min, 4 times/day) for a period of 28 days in the presence or absence of cytokines, interleukin-6 (IL-6) or tumor necrosis factor (TNF)α. Outcome assessments included histological and immunohistochemical staining of the explants; and the expression of …