Medicine and Health Sciences Commons^™

Obesity-Induced Inflammation In Human Mammary Tissue: A Potential Microenvironment Favorable To The Development Of Postmenopausal Breast Cancer Via The Wnt Signaling Pathway, Agathe A. Roubert

Masters Theses

In the United States, over one third of adult women are obese, and one in eight women will be diagnosed with breast cancer in their lifetime. Obesity has been shown to be a risk factor for postmenopausal breast cancer and is associated with increased aggressiveness and poor prognosis regardless of menopausal status. However, the mechanisms involved in the relationship between obesity and breast cancer are still not fully understood. Wnt signaling is often elevated in breast tumors (~60%) and is suspected to play a key role in cancer development. It has been shown that inflammatory cytokines, such as TNF-α, IL-1β, …

The Human Intruder Test: An Anxiety Assessment In Rhesus Macaques (Macaca Mulatta), Emily J. Peterson

Masters Theses

The human intruder test (HIT) is a noninvasive tool widely used for assessing anxiety in rhesus macaques (Macaca mulatta). This thesis explores the HIT procedure and applies it to a population of monkeys with a self-injurious behavioral pathology. Individual variation on this test can be used to assess anxiety and temperament. The first experiment of this thesis applied two different procedures of the HIT to 17 monkeys at UMass. Monkeys displayed little response to the intruder, and no significant differences were detected for the two procedures. To determine whether these responses were unique to the UMass monkeys, their …

Characterization Of The Reconstituted And Native Pseudomonas Aeruginosa Type Iii Secretion System Translocon, Kathryn R. Monopoli

Masters Theses

The Type III Secretion (T3S) system is a system utilized by many pathogenic bacteria to inject proteins into host cells during an infection. Effector proteins enter the host cell by passing through the proteinaceous T3S translocon, which forms a pore on the host cell membrane. Pseudomonas aeruginosa is an opportunistic pathogen that utilizes the T3S system, and very little is known about how the P. aeruginosa translocon forms.

The proteins PopB and PopD are believed to assemble into the P. aeruginosa translocon. A pore-forming heterocomplex of PopB and PopD has been reconstituted in model membranes, however this heterocomplex has not …

Differential Microglial Activation Following Immune Challenge In Peripubertal And Adult Outbred Mice, David J. Placzek

Masters Theses

Pubertal development is a time of growth and development in the brain, leading to high sensitivity during this period. Past research in our lab has shown that shipping female inbred and outbred mice during pubertal development alters their sensitivity to steroid hormones in adulthood, thus affecting sexual receptivity, cognition, depression-like behavior, and anxiety-like behavior. Here, we test the hypothesis that mice treated with lipopolysaccharide during pubertal development would have more active microglia, the brain's immune cells, after injection than mice treated with lipopolysaccharide in adulthood. No significant interactions were observed between treatment and age between any brain area measured, suggesting …

Associations Between Alexithymia And Executive Function In Younger And Older Adults, Gennarina Diane Santorelli

Masters Theses

The prevalence of alexithymia, a condition characterized by difficulties identifying and verbalizing one’s emotions, increases across the lifespan, with older adults reporting greater alexithymic features than young and middle-aged adults. This late-life increase in alexithymia may be the product of age-related decline in prefrontal brain circuitry implicated in emotional awareness and executive processes, notably in the anterior cingulate cortex (ACC). There is a dearth of research on the link between executive function and alexithymia in healthy adults. This study determined associations between alexithymia and executive function in healthy younger and older adults. Higher alexithymia scores were predicted to be associated …

Identifying Menstrual Symptom Patterns In Young Women Using Factor And Cluster Analysis, Felicia A. Quintana-Zinn

Masters Theses

Approximately 80% of reproductive age women experience physical or emotional symptoms prior to onset of menses. Of these women, approximately 20% experience symptoms severe enough to interfere with social functioning and life activities and meet criteria for premenstrual syndrome (PMS). More than 100 different symptoms are associated with PMS, the most common of which include breast tenderness, headache, anger, and depression. Symptom groupings tend to be stable within an individual but can vary distinctly between women. Potential differences in the etiology of symptoms suggest that PMS should not be considered a single condition in research or clinical studies, but rather …

The Effects Of Ovarian Hormones And Exercise On Gene Markers Of Cardiac Dysfunction, Anisha S. Patel

Masters Theses

Heart disease is the leading cause of death in women in the United States. Premenopausal women appear to have better cardiac function and lower risk of heart disease compared to male postmenopausal female counterparts. Ovarian hormone loss influences blood pressure homeostasis and causes systemic inflammation, which may result in chronic stress on the heart. Two key physiological changes in cardiac dysfunction are reemergence of the fetal gene pattern and myocardial remodeling. Physical activity has been linked to improved cardiac function. The purpose of this study was to investigate the effects of ovariectomy on early markers of cardiac dysfunction and fibrosis …

The Quality Of Life Among Adults With Autism Spectrum Disorder, Katharine F. Moroney

Masters Theses

Autism Spectrum Disorder (ASD) is a neurodevelopmental disorder that may affect several aspects of communication, including social and pragmatic functioning. There is a paucity of research in general involving adults with ASD, especially in the United States. Moreover, the strand of research that is significantly lacking involves the quality of life among adults with ASD. While considering the increase in the prevalence of ASD, it is important to empirically investigate the Quality of Life (QoL) of adults with ASD. The World Health Organization (WHO) defines QoL as, “the individual’s perception of their position in life… ranging from the person’s physical …

Do Word-Level Characteristics Predict Spontaneous Finiteness Marking In Specific Language Impairment?, Patrick S. Wilson

Masters Theses

The correct use of morphological suffixes in obligatory contexts reflects linguistic knowledge and competence of speakers. Grammatical knowledge is acquired during a child’s period of primary language acquisition, and may be partial or incomplete due to normal linguistic variation found during acquisition, due to a child’s level of progression through typical chronological development, or due to the presence of language disorders, like specific language impairment (SLI). In the current study, we ask whether characteristics of verbs make it more or less likely that children will correctly use an inflectional morpheme. The morphemes of interest in the current study were third …

Urinary Phthalate Metabolite Concentrations And Cancer Mortality In Nhanes, 1999-2006, A B. Kaiser

Masters Theses

Four in ten people in the US will be diagnosed with cancer during their lifetime. Environmental exposures are important determinants of cancer risk, causing as many as 19% of cancers worldwide. Phthalates are a group of chemicals used to increase the flexibility of plastics and vinyl in household materials such as food packaging, plastic toys, wood finishes and adhesives. Some phthalates may act as endocrine disruptors with hypothesized links to endometriosis, breast cancer, and reproductive outcomes. However, no research yet exists on phthalate exposure and all-cancer mortality. We investigated the relationship between seven urinary phthalate metabolites among 5,205 adults in …

Pre-Pregnancy Bmi And Preterm Birth Among Hispanic Teens, Allison C. Hope

Masters Theses

Preterm birth affects 12% of infants in the United States annually and is the main contributor to infant deaths and long-term neurological disabilities in offspring. Obesity is a growing problem in the U.S., and is increasingly being considered a major risk factor for adverse health outcomes. Puerto Rican teenagers have disproportionately high rates of preterm birth and obesity when compared to non-Hispanic White teenagers. Studies evaluating risk factors for preterm birth among adolescents are sparse, have inconsistent findings, and were conducted among predominantly non-Hispanic populations. Therefore, we investigated the association between BMI and preterm birth among the 419 teenage (ages …

Examining Implementation Of The Massachusetts Act Relative To Safety Regulations For School Athletic Programs (Sessions Laws: Chapter 166 Of The Acts Of 2010): A Multiple-Case Study, Mitchell L. Doucette

Masters Theses

Background/Purpose: Reducing the incidence and negative consequences of concussion among youth athletes is a public health priority. Fifty states have adopted legislation addressing the problem of sports-related concussions among youth-athletes. In 2010, Massachusetts adopted legislation based on Washington State’s Lystedt Law, enacting state-wide requirements for high school athletic programs. This study explored how the legislation has been implemented within Massachusetts schools and school-districts and identified factors influential to local implementation.

Methods: A qualitative multiple-case study approach was utilized. US Census data concerning the household median income and population size of the school-district’s representative town(s) were used to purposively recruit cases. …

Does Change In Physical Activity Predict Mental Health Outcomes In Pre-Adolescent African American Girls?, Sarah A. Burkart

Masters Theses

Pediatric obesity is a significant clinical and public health issue for African American girls in which low physical activity (PA) is a contributor. The mother-daughter relationship (MDR) has rarely been examined in the context of improving health behaviors such as PA and mental health outcomes (MHO) within this population. PURPOSE: To examine if change in PA following a 12-week culturally-tailored mother-daughter PA intervention predicts change in MHO variables (self-esteem, depressive symptoms, body image dissatisfaction) and MDR in pre-adolescent African American girls. METHODS: Mothers (n=27; age=36.0±17.0 years; body mass index (BMI)=34.0±7.4 kg/m²) and daughters (n=27; age=9.0±1.4 years; BMI=20.3±5.7 kg/m …

Thermo-Responsive Poly(N-Isopropylacrylamide) And Its Critical Solution Temperature Type Behavior In Presence Of Hydrophilic Ionic Liquids, Purnendu K. Nayak

Masters Theses

Thermo-responsive homopolymer poly(N-isopropylacrylamide), is a widely studied and used polymer. Our recent observations on thermal behavior of aqueous solutions of this polymer requires a short overview of existing results in order to understand the formation of different phases, both stable and unstable with the addition of hydrophilic Ionic liquids (ILs) 1-Butyl-3-methylimidazolium tetrafluoroborate ([BMIM][BF₄]), 1-Butyl-3-methylimidazolium acetate ([BMIM][OAc]) and 1-Butyl-3-methylimidazolium thiocyanate ([BMIM][SCN]) to the system. PNIPAM is soluble in cold water due to its inter- and intramolecular hydrogen bonding but phase separates upon heating at T > 32 , which is its lower critical solution temperature (LCST). PNIPAM exists in an …

Live Well Springfield – A Community Transformation Movement: Evaluation Of The Live Well Springfield Website, Jesse A. Mushenko

Masters Theses

The Live Well Springfield (LWS) movement is a collaborative effort of partner organizations in Springfield, Massachusetts. The project promotes healthy living by increasing knowledge and awareness of food and physical activity. A key LWS strategy was the creation of a website to function as an information hub. In addition to local event and health information, the website features 16 narratives depicting residents practicing healthy lifestyle choices, designed to encourage community engagement. To date, there has been no evaluation of the website’s reach and effect.

A mixed methods approach, surveys and focus group discussions, was designed to collect data from people …