Medicine and Health Sciences Commons^™

2,4-Disulfonyl Phenyl Butyl Nitrone, Its Salt And Their Use As Pharmaceuticals, John M. Carney

Pharmacology and Nutritional Sciences Faculty Patents

2,4-disulfonyl α-phenyl-tert-butyl nitrone and its pharmaceutically acceptable salts are disclosed. These materials are useful as pharmaceutical agents for oral or parenteral, e.g. intravenous administration to patients suffering from acute central nervous system oxidation as occurs in a stroke or from gradual central nervous system oxidation which can exhibit itself as progressive central nervous system function loss. The materials are also used to ameliorate the side effects of oxidative-damage causing antineoplastic disease treatments.

Phenyl Butyl Nitrone Compositions And Methods For Treatment Of Oxidative Tissue Damage, John M. Carney, Robert A. Floyd

Pharmacology and Nutritional Sciences Faculty Patents

Compositions for treating tissue damage from ischemia contain PBN, or active derivatives thereof, which are active during ischemia in preventing ATP depletion of the cells which predisposes them to subsequent injury during reperfusion, and which are active during reperfusion as oxygen radical scavengers, in a suitable pharmaceutical carrier for systemic or local administration, especially to the CNS, spinal column and eyes. Based on animal studies, the dosage for treating damage due to stroke is in the range of 10 to 300 mg/kg. Similar dosages are useful in treating damage resulting from free radical generation during inflammation, either as a product …

Logger Killed When Log Rolls Off Truck At Sawmill, Kentucky Injury Prevention And Research Center

Fatality Case Reports--Tractors/Logging

A 63-year-old male logger was struck by a log that rolled off a loaded truck at a sawmill. The victim was working with his two nephews that day and they were preparing to unload the logs from their two trucks. The logs were stacked at least twice as high as the standards on the sides of the flatbed trailers. As one nephew began to unchain the logs from his truck, the victim walked up to the truck, out of view of his nephew. When the chains were released, a log at the top of the load rolled off and struck …

Log Rolls Off Truck During Unloading And Strikes Logger, Kentucky Injury Prevention And Research Center

Fatality Case Reports--Tractors/Logging

A 52-year-old male logger was killed when he was struck by a log rolling off of his truck during the unloading process. The victim was a self-employed logger who had at least 25 years of experience. He was using the family-owned truck to haul a load of logs to the mill. The vertical stakes on the flatbed truck were approximately three feet high with the logs stacked another 3-4 feet above the stakes. He arrived at the mill at approximately 9am and began the unloading procedure. One of the chains that secured the load of logs was in two pieces …

Farm Worker Killed In Tractor Rollover On Public Roadway, Kentucky Injury Prevention And Research Center

Fatality Case Reports--Tractors/Logging

A 21-year-old farm worker was killed in a tractor rollover incident. The victim was driving a tractor on a secondary public road, pulling an unloaded 18-foot flatbed trailer, when he lost control. After the tractor wheels went off the right side of the road, the victim overcorrected, which caused the vehicle to become unbalanced; he was thrown from the tractor as it overturned into a ditch. It was not equipped with an appropriate rollover protective structure (ROPS) or a seatbelt. The unit, which included a canopy, had been installed to provide shade on summer days and cover in occasional downpours, …

Welder Is Crushed By An Unmanned Pipelayer, Kentucky Injury Prevention And Research Center

Fatality Case Reports--Other

A 34-year-old male welder was killed after being runover by an unmanned pipelayer. The victim worked for a pipelaying construction company as a contracted welder. On the day of the incident the victim, a welder's helper, and an equipment operator drove to the work site together to begin the day's activities. Access for the worksite was via a single lane temporary road winding up the mountainside through a mixed hardwood forest. The 1/2 mile dirt road carved its way up the hillside; on the left was the bank, on the right was a steep drop off. As the victim drove …

Polyamine-Polyamine And Polyamine-Protein Transport Inhibitor Conjugates And Their Use As Pharmaceuticals And In Research Relating To Polyamine Transport, Shewan M. Aziz, Mark N. Gillespie

Pharmaceutical Sciences Faculty Patents

Novel classes of inhibitors which selectively inhibit the cellular transport of normally transported substances, specifically polyamines are taught which comprise (i) polymers of the transported substance or (ii) protein or polypeptide conjugates of the transported substance. These inhibitors may be used in vitro to assess the effect of the transported substance on cellular functions and in vivo for treating disease conditions involving transport of the particular substance, e.g., a polyamine.

Logger Killed In Endloader Rollover, Kentucky Injury Prevention And Research Center

Fatality Case Reports--Tractors/Logging

A 41-year-old self-employed logger was killed when the endloader he was driving down a steep mountain road rolled over. Recent rainfall had made the dirt road wet and muddy. Tire marks left in the mud indicated that as the vehicle began to slide downhill, it hit the right embankment, causing it to veer to the left side of the road and roll over several times before coming to rest at the bottom of the dropoff on its left side. The victim was ejected from the vehicle and was found lying facedown about halfway between the edge of the roadway and …

Logger Killed By Falling Snag [95ky078], Kentucky Injury Prevention And Research Center

Fatality Case Reports--Tractors/Logging

A 22-year-old logger was killed after being struck on the head by a falling snag (dead standing tree). The victim was working with one co-worker, who was selectively cutting trees which were approximately 22 inches in diameter. Standing approximately three feet from the tree being cut, the victim was not wearing a hard hat. The cut tree broke a snag about 35 feet up, causing the snag to fall and strike the victim on the head. The co-worker (tree feller) shouted to the victim, but he was unable to move away in time to avoid being hit. FACE investigators concluded …

Farmer Crushed In Hay Baler, Kentucky Injury Prevention And Research Center

Fatality Case Reports--Tractors/Logging

A 32-year-old farmer was killed when he was crushed in a hay baler. The victim was operating a tractor, towing a round baler in an open field with gradually sloping terrain. Although the victim had been doing farm work since he was young and had experience operating square balers, his experience with this particular round baler was limited. The victim was found caught in the baler headfirst up to his waist. There were no witnesses to the incident. The victim was pronounced dead at the scene. In order to prevent similar incidents, the KY FACE investigators concluded that:

• PTO's should …

Involvement Of P59FynT In Interleukin-5 Receptor Signaling, Mark W. Appleby, James D. Kerner, Sylvia Chien, Charles R. Maliszewski, Subbarao Bondada, Roger M. Perlmutter

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Previous studies implicate the nonreceptor protein tyrosine kinase (PTK) p59fyn in the propagation of signals from the B cell antigen receptor. To elucidate the functions of this kinase, we examined B cell responsiveness in mice engineered to lack the hematopoietic isoform of p59fyn. Remarkably, antigen receptor signaling was only modestly defective in fyn^Tnull B cells. In contrast, signaling from the interleukin (IL)-5 receptor which ordinarily provides a comitogenic stimulus with antiimmunoglobulin, was completely blocked. Our results document the importance of p59fyn^T in IL-5 responses in B cells, and they support a general model …

Farmer Is Run Over By Disc After Falling From Tractor, Kentucky Injury Prevention And Research Center

Fatality Case Reports--Tractors/Logging

Because access to the scene and inspection of the equipment were not possible in this case, a partial inspection was done. Conclusions cannot be drawn from the evidence collected.

A 68-year-old farmer died when he fell from his tractor into the path of an attached disc. The victim had begun discing a tobacco field about 3:00 p.m., minutes after his wife and son left for second-shift work at a factory. After completing one field, he proceeded to a second field to complete the afternoon's work. The second field was across a small galley. As the victim drove the tractor through …

Apparatus And Method For Multiple Wavelength Of Tissue, Robert A. Lodder, Lisa A. Cassis

Pharmaceutical Sciences Faculty Patents

An apparatus for analyzing the chemical composition of tissue includes a near infrared light source, a fiber-optic probe, a mechanism for directing the light from the light source onto the tissue and detectors for detecting light reflected by the tissue. The light source transmits an incident beam having a wavelength ranging from 1000 to 2500 nm. A compound parabolic concentrator (CPC) is connected to a distal end of the fiber optic probe to focus or concentrate the wavelengths of light simultaneously and in parallel on a particular spot of tissue. Alternatively, an inverted, substantially conical reflector is positioned to scatter …

Farmer Killed In Tractor Rollover [95ky039], Kentucky Injury Prevention And Research Center

Fatality Case Reports--Tractors/Logging

A 66-year-old male farmer was killed in a tractor rollover incident. The victim was discing a small tobacco plot near his home when he lost control of his tractor. It went over an embankment, rolled one-half turn and landed on the victim before rolling further down the embankment. It was not equipped with a rollover protective structure (ROPS) or a seatbelt; its brakes were inoperable. The victim was pronounced dead at the scene by the county coroner. In order to prevent similar incidents, the KY FACE investigator concluded that:

• tractors should be retrofitted with Roll Over Protective Structures and seatbelts; …

Farmer Killed After Tractor Overturns Into Creek Bed, Kentucky Injury Prevention And Research Center

Fatality Case Reports--Tractors/Logging

A 47-year-old farmer/chemical analyst was killed when his tractor overturned into a muddy creek bed. The victim was checking a fence line on his property, which in that area has a rough, uneven terrain and on that day was very wet from a thunderstorm the previous night. He was going up an incline when his tractor slid backwards in the mud; the back tire slipped over the edge of a steep embankment and the tractor rolled over into the creek bed, pinning him face down in the mud. The tractor was not equipped with a Roll Over Protective Structure (ROPS) …

Farmer Run Over After Falling From Tractor, Kentucky Injury Prevention And Research Center

Fatality Case Reports--Tractors/Logging

A 54-year-old male farmer was killed in a tractor runover incident. The victim was on his way to his tobacco field when he lost control of his tractor. After it left the road, he fell and was run over by the right rear tire and possibly the edge of the attached plow. The three-year-old tractor was equipped with a seatbelt, but it was not fastened. The victim was pronounced dead at the scene by the county coroner. In order to prevent similar incidents, the KY FACE investigator concluded that:

• Tractor operators should always wear seatbelts;
• Tractor operators should pay close …

Identification Of An Idiotypic Peptide Recognized By Autoantibodies In Human Immunodeficiency Virus-1-Infected Individuals, Q. L. Wang, H.-T. Wang, Edwin Blalock, Sybille Müller, Heinz Köhler

Markey Cancer Center Faculty Publications

Antibodies against HIV-1 proteins in HIV-1-infected individuals share a cross-reactive idiotype defined by the monoclonal antiidiotypic antibody 1F7 (5). Using a computer algorithm based on the molecular recognition theory, regions of inverse hydropathy between the variable sequence of 1F7 and human monoclonal anti-HIV-1 antibodies were identified, which are assumed to be involved in idiotype-antiidiotype contacts. A peptide was designed from the proposed contact in the variable heavy chain framework 3-complementarity determining region 3 (FR3-CDR3) of human antibodies and was synthesized. This peptide is recognized by the antiidiotype 1F7 and inhibits the binding of 1F7 to human anti-HIV-1 antibodies which express …

Method For Inhibiting Angiogenesis With Aurintricarboxylic Acid, Its Analogues Or Salts, Delwood C. Collins, Antonio Gagliardi, Anjan Bhattacharyya

Obstetrics and Genecology Faculty Patents

Aurintricarboxylic acid has potent anti-angiogenic activity and may be used for new therapeutic approaches for diseases of neovascularization including the treatment of solid tumors, diabetic retinopathy and arthritis, among others.

Farm-Tractor-Related Fatalities -- Kentucky, 1994, T. W. Struttmann, Carl Spurlock, Susan H. Pollack, E. Moon-Hampton, Steven R. Browning, Robert Mcknight, Reginald Finger

Kentucky Injury Prevention and Research Center Faculty Publications

Fatalities associated with farm tractors are the most common cause of work-related death in the U.S. agricultural industry (1). To characterize farm-tractor-related fatalities in Kentucky, the Kentucky Fatality Assessment and Control Evaluation (KY FACE) Project studied all fatal farm injuries occurring among persons in that state during 1994, the initial year of operation for FACE in Kentucky. This report summarizes the results of that study.

Immune Response To The Carcinoembryonic Antigen In Patients Treated With An Anti-Idiotype Antibody Vaccine, Kenneth A. Foon, Mala Chakraborty, William J. John, Amanda Sherratt, Heinz Köhler, Malaya Bhattacharya-Chatterjee

Markey Cancer Center Faculty Publications

We have generated an IgG1 murine monoclonal anti-idiotype antibody (Ab2) designated 3H1, which mimics a specific epitope on the carcinoembryonic antigen (CEA). Patients with CEA positive tumors are immunologically "tolerant" to CEA. We used 3H1 as a surrogate for CEA for vaccine therapy of 12 patients with advanced colorectal cancer. Each of the patients received a minimum of four intracutaneous injections of aluminum hydroxide precipitated 3H1 at either 1, 2, or 4 mg dosage per injection. 9 of 12 patients demonstrated anti-anti-idiotypic (Ab3) response to 3H1. All nine patients generated specific anti-CEA antibody demonstrated by reactivity with radiolabeled purified CEA; …

Retiree Dies In Tractor Rollover, Kentucky Injury Prevention And Research Center

Fatality Case Reports--Tractors/Logging

A 63-year-old retired bus driver was pinned underneath a rear tractor tire after it overturned while he was bush hogging. The 37-year-old tricycle tractor, not equipped with a ROPS or a seatbelt, had a bush hog attached. On the morning of the incident, the operator and his brother were mowing a portion of a pasture. At about 9:30 am, the victim started mowing at the bottom of a steep slope, and then progressed toward the top of the ridge. In order to make turns he had to circle around three dogwood trees at the top of the ridge. His brother …

Non-Destructive Method For Radiolabelling Biomolecules By Halogenation, Anwar A. Hussain, Lewis W. Dittert

Pharmaceutical Sciences Faculty Patents

A kit and method of non-destructively radiolabelling a biomolecule substrate by halogenation, including the steps of: a) reacting a secondary amine with a chlorinating agent to produce an N-chloro secondary amine, (b) reacting the N-chloro secondary amine with a salt of a radiolabelled halogen to produce a radiolabelled halogenating agent, (c) reacting a solution of the biomolecule substrate with the radiolabelled halogenating agent, and (d) recovering a radiohalogenated biomolecule from the reaction of step (c). The kit and method allow for more complete iodination with a lessening of side reactions such as decomposition of the biomolecule substrate to be labelled.

86 Year Old Farmer Is Killed After Being Hit By Tree Branch, Kentucky Injury Prevention And Research Center

Fatality Case Reports--Tractors/Logging

An 86-year-old male farmer was killed after being struck by a tree limb. The victim was driving a tractor through a pasture checking on his cattle. As he approached a large tree, the extended front end loader, in full upright position, which was attached to the tractor struck a tree branch. The branch broke off and struck the victim causing massive head injury. He was thrown from the tractor and discovered a few hours later by his grandson. The tractor was equipped with a Roll Over Protective Structure (ROPS) and a non-function seat belt. The tractor did not have a …

Retiree/Part-Time Farmer Killed When Run Over By Bush Hog, Kentucky Injury Prevention And Research Center

Fatality Case Reports--Tractors/Logging

A 64-year-old male retiree and part-time farmer was killed when he fell from his tractor and was run over by the edge of the bush-hog attachment. The victim had been repairing a tractor tire earlier in the day. He was apparently trying to drive the tractor into a garage when he lost control and fell or was knocked from the tractor seat to the ground, falling between the tractor and the bush hog. The KY FACE investigator concluded that, in order to prevent similar occurrences, tractor operators should:

• Retrofit tractors with operator restraint systems (seatbelts) and Roll Over Protective Structures …

The Oral Physician... Creating A New Oral Health Professional For A New Century, David A. Nash

Oral Health Science Faculty Publications

No abstract provided.

Prison Employee Killed In Tractor Rollover, Kentucky Injury Prevention And Research Center

Fatality Case Reports--Tractors/Logging

A 36-year-old male prison recreation director was killed in a tractor rollover incident. The victim was driving the tractor down a sloping, S-curved driveway when he lost control. The tractor went over an embankment, rolled one-half turn, and came to rest on the victim. It was not equipped with a Rollover Protective Structure (ROPS) or a seatbelt. The victim was pronounced dead at the scene by the county coroner. In order to prevent similar incidents, the KY FACE investigators concluded that:

• Tractors should be retrofitted with Roll Over Protective Structures and seatbelts.
• Employers should provide tractor operation training by safety …

Farmer Killed When Run Over By Tractor In Barn, Kentucky Injury Prevention And Research Center

Fatality Case Reports--Tractors/Logging

A 62-year-old male farmer was killed inside his barn on a Saturday afternoon after starting his tractor from a standing position. The victim was demonstrating recent repairs to the tractor for his brother-in-law; a new battery and starter had been installed. Standing beside the tractor, the victim reached for the ignition switch to turn the tractor on. The tractor was in gear; when he turned the key the tractor lurched forward, running over the victim, who died moments later. The KY FACE investigators concluded that, to prevent similar occurrences, farmers and other tractor operators should:

• Never attempt to start a …

Pbn, Dmpo, And Pobn Compositions And Method Of Use Thereof For Inhibition Of Age-Associated Oxidation, John M. Carney, Robert A. Floyd

Pharmacology and Nutritional Sciences Faculty Patents

Compositions containing as the active ingredient a spin-trapping reagent, preferably α-phenyl butyl nitrone (PBN) or spin-trapping derivatives thereof, in a suitable pharmaceutical carrier for administration to a patient are disclosed for treating or preventin symptoms associated with aging or other conditions associated with oxidative tissue damage. Other spin-trapping agents can also be used, such as 5,5-dimethyl pyrroline N-oxide (DMPO) or α-(4-pyridyl 1-oxide)-N-tert-butylnitrone (POBN), and other spin-trapping derivatives thereof. These compositions and methods are useful in the treatment of age-related disorders, pre-surgical and/or pre-anesthetic preparation or administration of chemotherapeutic agents, and in the treatment of disorders or trauma of the brain, …

Apparatus And Method For Noninvasive Chemical Analysis, Robert A. Lodder

Pharmaceutical Sciences Faculty Patents

A method and apparatus are provided for magnetohydrodynamic acoustic-resonance, near-IR spectroscopy. The method includes a step of applying to a subject under study a magnetic field having a strength between 2,00-10,000 gauss, near-IR radiation having a wavelength between 800-3,000 nm and an acoustic wave having a frequency between 10 khz-1 Mhz. The method also includes the steps of inducing vibration of ions in the magnetic field and detecting an electric wave generated magnetohydrodynamically by the acoustic wave induced vibration of the ions. Next is the collecting of the electrical, acoustical and near-IR spectra and the analyzing of the collected spectra. …

Tissue-Specific Expression Of Human Lipoprotein Lipase: Effect Of The 3′-Untranslated Region On Translation, Gouri Ranganathan, John M. Ong, Ada Yukht, Mehrnoosh Saghizadeh, Rosa B. Simsolo, Andrea Pauer, Philip A. Kern

Clinical and Translational Science Faculty Publications

Lipoprotein lipase (LPL) is a central enzyme in lipoprotein metabolism and is expressed predominantly in adipose tissue and muscle. In these tissues, the regulation of LPL is complex and often opposite in response to the same physiologic stimulus. In addition, much regulation of LPL occurs post-transcriptionally. The human LPL cDNA is characterized by a long 3′-untranslated region, which has two polyadenylation signals. In this report, human adipose tissue expressed two LPL mRNA species (3.2 and 3.6 kb) due to an apparent random choice of sites for mRNA polyadenylation, whereas human skeletal and heart muscle expressed predominantly the longer 3.6-kb mRNA …