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Embryonic Origins Of A Motor System: Motor Dendrites Form A Myotopic Map In Drosophila, Matthias Landgraf, Victoria Jeffrey, Miki Fujioka, James B. Jaynes, Michael Bate

Department of Biochemistry and Molecular Biology Faculty Papers

The organisational principles of locomotor networks are less well understood than those of many sensory systems, where in-growing axon terminals form a central map of peripheral characteristics. Using the neuromuscular system of the Drosophila embryo as a model and retrograde tracing and genetic methods, we have uncovered principles underlying the organisation of the motor system. We find that dendritic arbors of motor neurons, rather than their cell bodies, are partitioned into domains to form a myotopic map, which represents centrally the distribution of body wall muscles peripherally. While muscles are segmental, the myotopic map is parasegmental in organisation. It forms …

Characterization Of Mutations And Loss Of Heterozygosity Of P53 And K-Ras2 In Pancreatic Cancer Cell Lines By Immobilized Polymerase Chain Reaction, James Butz, Eric Wickstrom, Jeremy Edwards

Department of Biochemistry and Molecular Biology Faculty Papers

Background

The identification of known mutations in a cell population is important for clinical applications and basic cancer research. In this work an immobilized form of the polymerase chain reaction, referred to as polony technology, was used to detect mutations as well as gene deletions, resulting in loss of heterozygosity (LOH), in cancer cell lines. Specifically, the mutational hotspots in p53, namely codons 175, 245, 248, 249, 273, and 282, and K-ras2, codons 12, 13 and 61, were genotyped in the pancreatic cell line, Panc-1. In addition LOH analysis was also performed for these same two genes in Panc-1 by …