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Full-Text Articles in Medicine and Health Sciences
Gene Expression Analysis Of Bovine Embryonic Disc, Trophoblast And Parietal Hypoblast At The Start Of Gastrulation, P Pfeffer, C Smith, P Maclean, D Berg
Gene Expression Analysis Of Bovine Embryonic Disc, Trophoblast And Parietal Hypoblast At The Start Of Gastrulation, P Pfeffer, C Smith, P Maclean, D Berg
Medical Papers and Journal Articles
In cattle early gastrulation-stage embryos (Stage 5), four tissues can be discerned: (i) the top layer of the embryonic disc consisting of embryonic ectoderm (EmE); (ii) the bottom layer of the disc consisting of mesoderm, endoderm and visceral hypoblast (MEH); (iii) the trophoblast (TB); and (iv) the parietal hypoblast. We performed microsurgery followed by RNA-seq to analyse the transcriptome of these four tissues as well as a developmentally earlier pre-gastrulation embryonic disc. The cattle EmE transcriptome was similar at Stages 4 and 5, characterised by the OCT4/SOX2/NANOG pluripotency network. Expression of genes associated with primordial germ cells suggest their presence …