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Full-Text Articles in Medicine and Health Sciences
A Pilot Study For Early Detection Of Oral Premalignant Diseases Using Oral Cytology And Raman Micro-Spectroscopy: Assessment Of Confounding Factors, Isha Behl, Genecy Calado, Alison Malkin, Stephen Flint, Sheila Galvin, Claire Healy, Marina Leite Pimentel, Hugh Byrne, Fiona Lyng
A Pilot Study For Early Detection Of Oral Premalignant Diseases Using Oral Cytology And Raman Micro-Spectroscopy: Assessment Of Confounding Factors, Isha Behl, Genecy Calado, Alison Malkin, Stephen Flint, Sheila Galvin, Claire Healy, Marina Leite Pimentel, Hugh Byrne, Fiona Lyng
Articles
This study demonstrates the efficacy of Raman micro-spectroscopy of oral cytological samples for differentiating dysplastic, potentially malignant lesions from those of normal, healthy donors. Cells were collected using brush biopsy from healthy donors (n = 20) and patients attending a Dysplasia Clinic (n = 20). Donors were sampled at four different sites (buccal mucosa, tongue, alveolus, gingiva), to ensure matched normal sites for all lesions, while patient samples were taken from clinically evident, histologically verified dysplastic lesions. Spectra were acquired from the nucleus and cytoplasm of individual cells of all samples and subjected to partial least squares-discriminant analysis. Discriminative sensitivities …
Raman Microspectroscopic Study For The Detection Of Oral Field Cancerisation Using Brush Biopsy Samples, Isha Behl, Genecy Calado, Anika Vishwakarma, Stephen Flint, Sheila Galvin, Claire Healy, Marina Leite Pimentel, Alison Malkin, Hugh Byrne, Fiona Lyng
Raman Microspectroscopic Study For The Detection Of Oral Field Cancerisation Using Brush Biopsy Samples, Isha Behl, Genecy Calado, Anika Vishwakarma, Stephen Flint, Sheila Galvin, Claire Healy, Marina Leite Pimentel, Alison Malkin, Hugh Byrne, Fiona Lyng
Articles
Field cancerisation (FC) is potentially an underlying cause of poor treatment outcomes of oral squamous cell carcinoma (OSCC). To explore the phenomenon using Raman microspectroscopy, brush biopsies from the buccal mucosa, tongue, gingiva and alveolus of healthy donors (n = 40) and from potentially malignant lesions (PML) of Dysplasia Clinic patients (n = 40) were examined. Contralateral normal samples (n = 38) were also collected from the patients. Raman spectra were acquired from the nucleus and cytoplasm of each cell, and subjected to partial least squares-discriminant analysis (PLS-DA). High discriminatory accuracy for donor and PML samples was achieved for both …