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Full-Text Articles in Medicine and Health Sciences
Effects Of Centrifugation And Washing Of Freeze-Thawed Blood On Isolated Dna Characteristics, Mevlüt Arslan
Effects Of Centrifugation And Washing Of Freeze-Thawed Blood On Isolated Dna Characteristics, Mevlüt Arslan
Turkish Journal of Veterinary & Animal Sciences
DNA isolations from the whole blood are commonly performed to obtain DNA for molecular research and diagnostics. Generally, blood samples are taken into anticoagulant tubes and stored in deep freezers until DNA isolation. In fresh blood, pretreatments or leukocytes preparations can be performed and suggested for advanced DNA isolation. However, similar applications in freeze-thawed blood (FTB) have not been shown yet. In the study, centrifugation and washing of FTB were applied as pretreatment before DNA isolations, and their effects on isolated DNA characteristics including DNA integrity, quality, quantity, mitochondrial (mt), and nuclear (n) DNA levels were investigated. Microscopic and flow …
Breed, Age, And Sex-Related Variations In Hematological And Some Biochemicalparameters In The Tornjak Dog, Ivona Alilovic, Dunja Rukavina, Atifa Ajanovic, Cazim Crnkic, Husein Ohran, Amir Zahirovic
Breed, Age, And Sex-Related Variations In Hematological And Some Biochemicalparameters In The Tornjak Dog, Ivona Alilovic, Dunja Rukavina, Atifa Ajanovic, Cazim Crnkic, Husein Ohran, Amir Zahirovic
Turkish Journal of Veterinary & Animal Sciences
There is an increasing significance for the breed, age, and sex-related variations in hematological and biochemical parameters in veterinary medicine. The primary objective of this study was to identify possible breed-related differences and to determine the effect of age and sex on hematologic and serum biochemical parameters in the Bosnian and Herzegovinian - Croatian Shepherd dog Tornjak raised in Bosnia and Herzegovina (B&H). Data from 19 hematological and 17 serum biochemical parameters were analyzed in the group of 100 dogs (47 females and 53 males), sexually intact and aged between one month and twelve years. Breed affected TP (total protein) …
A Comparative Study Of Blood And Milk Chemiluminescence In Healthy Cows And Withsubclinical And Clinical Mastitis, Radoslaw Skorzewski, Wieslaw Krumrych, Hanna Markiewicz, Elzbieta Sandurska
A Comparative Study Of Blood And Milk Chemiluminescence In Healthy Cows And Withsubclinical And Clinical Mastitis, Radoslaw Skorzewski, Wieslaw Krumrych, Hanna Markiewicz, Elzbieta Sandurska
Turkish Journal of Veterinary & Animal Sciences
The aim of the study was to evaluate the luminol-dependent spontaneous and stimulated chemiluminescence (CL) of whole blood and milk in cows with subclinical and clinical mastitis. The research was carried out on 108 Holstein-Friesian dairy cows aged 3-5 years. Based on the assessment of somatic cell count (SCC) in quarter milk, the animals were divided into 3 groups: I - clinically healthy cows (n = 35), II - animals with subclinical mastitis (n = 30) and III - cows with clinical mastitis (n = 43). The material for the research was whole blood and quarter milk. The samples were …