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Full-Text Articles in Medicine and Health Sciences
A Comparison Of The Effect Of Methyl-B-Cyclodextrin On The Osmotic Fragility Of Ovine, Bovine And Human Erythrocytes, Şevket Arikan
A Comparison Of The Effect Of Methyl-B-Cyclodextrin On The Osmotic Fragility Of Ovine, Bovine And Human Erythrocytes, Şevket Arikan
Turkish Journal of Veterinary & Animal Sciences
Cyclodextrins are used extensively in food, cosmetic, drug and chemical industries to increase aqueous solubility and stability, and reduce or eliminate the unpleasant taste and smell of many products. Experiments were conducted to examine the effect of a pre-haemolytic dose of methyl b-cyclodextrin (MBCD) on the osmotic fragility of erythrocytes collected from sheep, cattle and humans. Heparinised blood collected from these three species were analysed within 3 h of collection. Erythrocyte suspensions were mixed with MBCD to give concentrations of 2 mM, 4 mM or 6 mM cyclodextrin. The mixtures were incubated for 30 min at 37 °C and centrifuged. …
Osmotic Fragility And Fatty Acid Composition Of Erythrocyte In Mirror Carp (Cyprinus Carpio) Kept At Different Water Temperatures, Olcay Hi̇sar, Şükri̇ye Aras Hi̇sar, Mükerrem Kaya, Telat Yanik
Osmotic Fragility And Fatty Acid Composition Of Erythrocyte In Mirror Carp (Cyprinus Carpio) Kept At Different Water Temperatures, Olcay Hi̇sar, Şükri̇ye Aras Hi̇sar, Mükerrem Kaya, Telat Yanik
Turkish Journal of Veterinary & Animal Sciences
Osmotic fragility and fatty acid composition in erythrocytes of mirror carp (Cyprinus carpio) kept at 8 °C, 16 °C and 28 °C were determined. Osmotic fragility in erythrocytes (EOF) increased significantly (0.19-0.24%) with the low water temperature. Significant declines in the quantity of saturated fatty acid (44.49-38.40%) and monounsaturated fatty acids (26.30-13.99%), and significant increases in that of polyunsaturated fatty acid (29.21-47.59%) were determined (P < 0.05). It was concluded that low water temperature caused a tolerable stress in mirror carps.
Purification Of Glucose 6-Phosphate Dehydrogenase From Goose Erythrocytes And Kinetic Properties, Şükrü Beydemi̇r, Hayrullah Yilmaz, Mehmet Çi̇ftçi̇, Ebubeki̇r Bakan, Ömer İrfan Küfrevi̇oğlu
Purification Of Glucose 6-Phosphate Dehydrogenase From Goose Erythrocytes And Kinetic Properties, Şükrü Beydemi̇r, Hayrullah Yilmaz, Mehmet Çi̇ftçi̇, Ebubeki̇r Bakan, Ömer İrfan Küfrevi̇oğlu
Turkish Journal of Veterinary & Animal Sciences
Glucose 6-phosphate dehydrogenase (G6PD) was purified from goose erythrocytes and some characteristics of the enzyme were investigated. The purification procedure was composed of 3 steps: hemolysate preparation, ammonium sulfate precipitation, and 2', 5'-ADP Sepharose 4B affinity gel chromatography. Thanks to the 3 consecutive procedures, the enzyme, having a specific activity of 36.2 EU/mg protein, was purified for a yield of 68.79% and 3892 folds; to ascertain enzyme purity, SDS-PAGE was performed. Optimal pH, stable pH, optimal temperature, molecular weight, and K_m and V_{max} values for NADP^+ and glucose 6-phosphate (G6-P) substrates were also determined for the enzyme. In addition, K_i …