Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 13 of 13
Full-Text Articles in Medicine and Health Sciences
Assessment Of Erythrocyte Morphology In Patients With Type 2 Diabetes Mellitus: A Pilotstudy Of Electron Microscopy-Based Analysis In Relation To Healthy Controls, Tülay Mortaş, Şenay Arikan Durmaz, Şaban Cem Sezen, Yasemi̇n Savranlar
Assessment Of Erythrocyte Morphology In Patients With Type 2 Diabetes Mellitus: A Pilotstudy Of Electron Microscopy-Based Analysis In Relation To Healthy Controls, Tülay Mortaş, Şenay Arikan Durmaz, Şaban Cem Sezen, Yasemi̇n Savranlar
Turkish Journal of Medical Sciences
Background/aim: The present study aimed to assess erythrocyte morphology in newly diagnosed type 2 diabetes mellitus patients using scanning electron microscopy. Materials and methods: In total, 30 patients admitted to endocrine outpatient clinics were included in the study. The patients were divided into two groups according to their fasting blood glucose levels: type 2 diabetes mellitus (n = 15, fasting blood glucose levels ≥ 126 mg/dL) and control (n = 15, fasting blood glucose levels < 99 mg/dL). The patient's demographic characteristics, haemoglobin A1c levels, and scanning electron microscopy findings regarding erythrocyte morphology were recorded. Results: There was no significant difference between the control and type 2 diabetes mellitus group in terms of the participants' age (51.13 ± 8.53 vs. 50.33 ± 8.72 years, p = 0.8) and the male/female ratio (9/6 vs. 9/6). In the control group, discocytes were abundant, echinocytes were rare, and spherocytes were absent. On the other hand, discocytes were less common and echinocyte-shaped erythrocytes were more common in the type 2 diabetes mellitus group than in the control group. In addition, spherocytes were detected in the type 2 diabetes mellitus group. Moreover, the diameter of discocytes was significantly lower (p = 0.014), and blood glucose and haemoglobin A1c levels were significantly higher (p < 0.05 for both) in the type 2 diabetes mellitus group than in the control group. Conclusion: Our findings indicate that high glucose levels in type 2 diabetes mellitus patients lead to significant alterations in erythrocyte morphology, including decreased erythrocyte deformability and the formation of echinocytes and spherocytes due to eryptosis. The possibility of decreased erythrocyte deformability due to excessive eryptosis may disturb microcirculation in newly diagnosed, treatment-naïve type 2 diabetes mellitus patients who do not have any complications.
The Effects Of Prestorage Leukoreduction And Storage Duration On The In Vitro Quality Of Canine Packed Red Blood Cells, Eli̇f Ergül Eki̇z, Murat Arslan, İbrahi̇m Akyazi, Evren Eraslan Uygur, Güldal İnal Gülteki̇n, Mukaddes Özcan
The Effects Of Prestorage Leukoreduction And Storage Duration On The In Vitro Quality Of Canine Packed Red Blood Cells, Eli̇f Ergül Eki̇z, Murat Arslan, İbrahi̇m Akyazi, Evren Eraslan Uygur, Güldal İnal Gülteki̇n, Mukaddes Özcan
Turkish Journal of Veterinary & Animal Sciences
The effects of prestorage leukoreduction and storage duration on the in vitro quality parameters of canine packed red blood cells (pRBCs) stored in saline-adenine-glucose-mannitol (SAGM) were investigated. Hematological, biochemical, and morphological changes in filtered and unfiltered pRBCs were determined each week during 42 days of storage. All of the parameters changed significantly during storage. The effects of leukoreduction on leukocyte and erythrocyte count, packed cell volume, hemoglobin content, and erythrocyte morphology were significant. While supernatant K increased during storage, glucose, Cl, and 2,3-diphosphoglycerate (2,3-DPG) concentrations decreased. Filtered pRBCs had higher 2,3-DPG levels throughout storage than their unfiltered counterparts. It was …
The Effects Of Melatonin Hormone On Carbonic Anhydrase Enzyme Activity In Rainbow Trout (Oncorhynchus Mykiss) Erythrocytes In Vitro And In Vivo, Olcay Hi̇sar, Şükrü Beydemi̇r, İlhami̇ Gülçi̇n, Şükri̇ye Aras Hi̇sar, Telat Yanik, Ömer İrfan Küfrevi̇oğlu
The Effects Of Melatonin Hormone On Carbonic Anhydrase Enzyme Activity In Rainbow Trout (Oncorhynchus Mykiss) Erythrocytes In Vitro And In Vivo, Olcay Hi̇sar, Şükrü Beydemi̇r, İlhami̇ Gülçi̇n, Şükri̇ye Aras Hi̇sar, Telat Yanik, Ömer İrfan Küfrevi̇oğlu
Turkish Journal of Veterinary & Animal Sciences
The effects of melatonin hormone on rainbow trout (Oncorhynchus mykiss) erythrocyte carbonic anhydrase enzyme purified by using Sepharose 4B-L tyrosine-sulfanylamide affinity gel chromatography were investigated in vitro and in vivo. Carbonic anhydrase from Oncorhynchus mykiss erythrocytes was purified with a specific activity of ~400 EU/mg protein and ~200-fold in a yield of ~20. Melatonin did not inhibit carbonic anhydrase enzyme in vitro. Carbonic anhydrase activity of the control group was determined as 1597.7 ± 429.0 EU/g hemoglobin in vivo. Carbonic anhydrase activities were measured at 1, 3 and 5 h by following melatonin injection. The corresponding mean activities of melatonin …
6-Phosphogluconate Dehydrogenase: Purification, Characterization And Kinetic Properties From Rat Erythrocytes, Şükrü Beydemi̇r, Mehmet Çi̇ftçi̇, Hayrullah Yilmaz, Ömer İrfan Küfrevi̇oğlu
6-Phosphogluconate Dehydrogenase: Purification, Characterization And Kinetic Properties From Rat Erythrocytes, Şükrü Beydemi̇r, Mehmet Çi̇ftçi̇, Hayrullah Yilmaz, Ömer İrfan Küfrevi̇oğlu
Turkish Journal of Veterinary & Animal Sciences
In this paper, a simple and rapid method for the purification of 6-phosphogluconate dehydrogenase from rat erythrocytes together with an analysis of the kinetic behavior and some properties of the enzyme are considered. The purification steps comprised high-speed centrifugation, 20-50% ammonium sulfate precipitation and 2', 5'-ADP Sepharose 4B affinity gel chromatography. The yield was 78.4% and the specific enzyme activity was 5.15 EU/mg proteins. The molecular mass of the subunit was estimated to be 59,566 Da by SDS polyacrylamide gel electrophoresis (SDS-PAGE) and native enzyme was found to be 111,000 Da by gel filtration column chromatography. The enzyme had an …
A Comparison Of The Effect Of Methyl-B-Cyclodextrin On The Osmotic Fragility Of Ovine, Bovine And Human Erythrocytes, Şevket Arikan
A Comparison Of The Effect Of Methyl-B-Cyclodextrin On The Osmotic Fragility Of Ovine, Bovine And Human Erythrocytes, Şevket Arikan
Turkish Journal of Veterinary & Animal Sciences
Cyclodextrins are used extensively in food, cosmetic, drug and chemical industries to increase aqueous solubility and stability, and reduce or eliminate the unpleasant taste and smell of many products. Experiments were conducted to examine the effect of a pre-haemolytic dose of methyl b-cyclodextrin (MBCD) on the osmotic fragility of erythrocytes collected from sheep, cattle and humans. Heparinised blood collected from these three species were analysed within 3 h of collection. Erythrocyte suspensions were mixed with MBCD to give concentrations of 2 mM, 4 mM or 6 mM cyclodextrin. The mixtures were incubated for 30 min at 37 °C and centrifuged. …
Purification Of Glucose 6-Phosphate Dehydrogenase From Goose Erythrocytes And Kinetic Properties, Şükrü Beydemi̇r, Hayrullah Yilmaz, Mehmet Çi̇ftçi̇, Ebubeki̇r Bakan, Ömer İrfan Küfrevi̇oğlu
Purification Of Glucose 6-Phosphate Dehydrogenase From Goose Erythrocytes And Kinetic Properties, Şükrü Beydemi̇r, Hayrullah Yilmaz, Mehmet Çi̇ftçi̇, Ebubeki̇r Bakan, Ömer İrfan Küfrevi̇oğlu
Turkish Journal of Veterinary & Animal Sciences
Glucose 6-phosphate dehydrogenase (G6PD) was purified from goose erythrocytes and some characteristics of the enzyme were investigated. The purification procedure was composed of 3 steps: hemolysate preparation, ammonium sulfate precipitation, and 2', 5'-ADP Sepharose 4B affinity gel chromatography. Thanks to the 3 consecutive procedures, the enzyme, having a specific activity of 36.2 EU/mg protein, was purified for a yield of 68.79% and 3892 folds; to ascertain enzyme purity, SDS-PAGE was performed. Optimal pH, stable pH, optimal temperature, molecular weight, and K_m and V_{max} values for NADP^+ and glucose 6-phosphate (G6-P) substrates were also determined for the enzyme. In addition, K_i …
Osmotic Fragility And Fatty Acid Composition Of Erythrocyte In Mirror Carp (Cyprinus Carpio) Kept At Different Water Temperatures, Olcay Hi̇sar, Şükri̇ye Aras Hi̇sar, Mükerrem Kaya, Telat Yanik
Osmotic Fragility And Fatty Acid Composition Of Erythrocyte In Mirror Carp (Cyprinus Carpio) Kept At Different Water Temperatures, Olcay Hi̇sar, Şükri̇ye Aras Hi̇sar, Mükerrem Kaya, Telat Yanik
Turkish Journal of Veterinary & Animal Sciences
Osmotic fragility and fatty acid composition in erythrocytes of mirror carp (Cyprinus carpio) kept at 8 °C, 16 °C and 28 °C were determined. Osmotic fragility in erythrocytes (EOF) increased significantly (0.19-0.24%) with the low water temperature. Significant declines in the quantity of saturated fatty acid (44.49-38.40%) and monounsaturated fatty acids (26.30-13.99%), and significant increases in that of polyunsaturated fatty acid (29.21-47.59%) were determined (P < 0.05). It was concluded that low water temperature caused a tolerable stress in mirror carps.
Reference Values For Erythrocyte And Liver Antioxidant Systems In Healthy Male Mice (Mus Musculus), Ergül Belge Kurutaş, Fi̇gen Doran, Seyhan Vari̇nli̇
Reference Values For Erythrocyte And Liver Antioxidant Systems In Healthy Male Mice (Mus Musculus), Ergül Belge Kurutaş, Fi̇gen Doran, Seyhan Vari̇nli̇
Turkish Journal of Veterinary & Animal Sciences
The aim of the present study was to define the reference values for erythrocyte and liver tissue glucose-6-phosphate dehydrogenase (G6PDH), glutathione peroxidase (GPX), glutathione reductase (GR) and superoxide dismutase (SOD) activities and also reduced glutathione (GSH) levels in order to obtain basal data for subsequent toxicological investigations. In addition, the best storage conditions were determined for liver G6PDH enzyme. Male, apparently healthy Mus musculus (38) speciemens that had normal livers (histopathological data) were included in the study. G6PDH and GPX activities and GSH levels were measured by the Beutler method, and SOD aktivity was measured by the Fridovich method in …
The Effects Of Vitamin E On Some Blood Parameters In Broilers, Murat Arslan, Mukaddes Özcan, Erdal Matur, Ülker Çöteli̇oğlu, Eli̇f Ergül
The Effects Of Vitamin E On Some Blood Parameters In Broilers, Murat Arslan, Mukaddes Özcan, Erdal Matur, Ülker Çöteli̇oğlu, Eli̇f Ergül
Turkish Journal of Veterinary & Animal Sciences
The purpose of this study was to determine the effects of different doses of vitamin E supplemented into the diet on erythrocyte osmotic fragility and some biochemical parameters. Sixty broilers were randomly divided into four groups of 15 each (one control and three experimental groups). Experimental groups (D1, D2, D3) were fed with a diet supplemented with 100, 200 and 300 ppm of vitamin E respectively. The plasma vitamin E level, and other blood parameters, of all the test subjects were measured in the fifth and seventh week of the study. The plasma vitamin E level of D2 and D3 …
Relationship Between Aging And Susceptibility To Oxidative Damage: An Assessment By Erythrocyte Membrane Proteins And Lipids, Ahmet Özaydin, İlhan Onaran, Kadi̇r Avşar, Vecdet Tezcan, Gönül Sultuybek
Relationship Between Aging And Susceptibility To Oxidative Damage: An Assessment By Erythrocyte Membrane Proteins And Lipids, Ahmet Özaydin, İlhan Onaran, Kadi̇r Avşar, Vecdet Tezcan, Gönül Sultuybek
Turkish Journal of Medical Sciences
Contradictory results have been reported from various studies regarding the effect of age on susceptibility to oxidative damage. Using different parameters, we carried out a comparative study on the erythrocytes and erythrocyte membranes of healthy elderly and young adults to determine how resistance to oxidative damage is affected by aging. Thin layer chromatography and polyacrylamide gel electrophoresis under denaturing conditions were used to estimate changes in membrane phospholipids and cytoskeletal proteins, respectively. Our results showed that these components of intact erythrocytes incubated with cumene hydroperoxide (CumOOH), a powerful oxidising agent, remained unchanged in both age groups. However, exposure of erythrocyte …
Effect Of Exercise On Blood Antioxidant Status And Erythrocyte Lipid Peroxidation: Role Of Dietary Supplementation Of Vitamin E, Mustafa Kelle, Hüda Di̇ken, Abdurrahman Şermet, Mukadder Atmaca, Cemi̇l Tümer
Effect Of Exercise On Blood Antioxidant Status And Erythrocyte Lipid Peroxidation: Role Of Dietary Supplementation Of Vitamin E, Mustafa Kelle, Hüda Di̇ken, Abdurrahman Şermet, Mukadder Atmaca, Cemi̇l Tümer
Turkish Journal of Medical Sciences
We tested the effects of moderate physical exercise on the blood antioxidant capacity and erythrocyte lipid peroxidation in 21 Wistar albino rats. Erythrocyte superoxide dismutase (SOD) activity increased significantly (p
Oxidative Damage In Erythrocytes During Cold Storage With Organ Preservation Solution, Aki̇f B. Memmedoğlu, Ahmet Özaydin, İsmai̇l Seçki̇n, Gönül Sultuybek
Oxidative Damage In Erythrocytes During Cold Storage With Organ Preservation Solution, Aki̇f B. Memmedoğlu, Ahmet Özaydin, İsmai̇l Seçki̇n, Gönül Sultuybek
Turkish Journal of Medical Sciences
It is known that erythrocyte aggregation in renal tissue during preserva-tion is cause of microcirculation defects in the reperfusion period. The aim of our study is to investigate oxidative damage in erythrocytes relative to the time of cold ischemia during organ preservation and relationship between lipid peroxidation and development of these damages. In experiments with a rabbit model, explanted kidneys were exposed to perfusion and 96 hours preservation with Euro-Collins (EC) in the 1st group, and University of Wisconsin (UW) solution in the 2nd group. Electron-microscopic examinations in renal tissueswere performed. Lipid peroxidation and glutathione levels in erythrocytes preserved in …
Erythrocyte, Saliva And Serum Arginase Activity With Serum Urea And Estrogen Levels Inakkaraman And Ivesi Sheep In Pregnancy And Postpartum, Sema Temi̇zer Ozan, Sema Yaralioğlu Gürgöze, Tülay İleri̇ Büyükoğlu, İhsan Hali̇feoğlu
Erythrocyte, Saliva And Serum Arginase Activity With Serum Urea And Estrogen Levels Inakkaraman And Ivesi Sheep In Pregnancy And Postpartum, Sema Temi̇zer Ozan, Sema Yaralioğlu Gürgöze, Tülay İleri̇ Büyükoğlu, İhsan Hali̇feoğlu
Turkish Journal of Veterinary & Animal Sciences
Arginase (L-arginine amidino hydrolase E.C. 3.5.3.1) is an enzyme catalyzing the last stage of the Krebs-Henseleit cycle and, thus, converting arginine into urea and ornithine. Arginase also plays a part in protein and polyamine synthesis and is affected by hormones in terms of metabolic status. In the present study, erythrocyte, saliva, and serum arginase activity together with serum urea and estrogen levels were examined in Akkaraman and Ivesi sheep in pregnancy and postpartum. Comparisons were then made between the species. In pregnancy the urea level in saliva increased. However, the urea level in serum decreased. No clear difference was observed …