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Optimization And Application Of Metabolomic Assays For Analyzing Diet-Induced And Gut Microbiota-Derived Short-Chain Fatty Acids In Mice And Humans, Robert M. Juenemann

Electronic Theses and Dissertations

Introduction: In recent decades, the obesity epidemic worldwide has prompted the need for research targeting disease prevention, treatment, and maintenance. Dietary interventions are one of the primary methods to instill positive nutrition habits into one’s lifestyle. Thus, resistant starch type 4 (RS4), a prebiotic dietary fiber, has been proposed to induce beneficial immunometabolic health outcomes. Currently there is a lack of knowledge on the health outcomes of RS4 in adults with metabolic syndrome (MetS). Goal: The goal of this research was to optimize a metabolomic assay to quantify fecal short chain fatty acids (SCFAs), a byproduct of microbial fermentation …