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Articles 1 - 30 of 68
Full-Text Articles in Medicine and Health Sciences
Sociodemographic Characteristics, Clinicopathological Parameters And Survival Among Malaysians With Oral Squamous Cell Carcinoma (Oscc), Ghani W. M. N., Rosnah Binti Zain, Jalil N., Razak I. A., Raja-Latifah R. J., Samsuddin A. R.
Sociodemographic Characteristics, Clinicopathological Parameters And Survival Among Malaysians With Oral Squamous Cell Carcinoma (Oscc), Ghani W. M. N., Rosnah Binti Zain, Jalil N., Razak I. A., Raja-Latifah R. J., Samsuddin A. R.
Prof. Dr. Rosnah Binti Zain
Introduction: Western researchers showed that oral squamous cell carcinoma (OSCC) patients exhibit lower survival rate and poorer prognosis compared to other type of cancer where several clinicopathological parameters have been implicated in the survival of patients. This study aims to assess the association between sociodemographic and clinicopathological parameters with survival of OSCC patients. Method: Data were collected from 253 histologically diagnosed OSCC patients attending selected clinics nationwide. Survival was analyzed for relation with habits, site, disease stage and nodal involvement. Results: Mean age was 58.9 ± 12.6 years with 8.3% aged less than 40. Majority is females (61.1%) and of …
Mdm2 Splice Variants In Oral Cancer, Rosnah Binti Zain
Mdm2 Splice Variants In Oral Cancer, Rosnah Binti Zain
Prof. Dr. Rosnah Binti Zain
Introduction: MDM2 is a negative regulator of the p53 tumor suppressor protein. The presence of a variety of MDM2 splice variants have been reported in many different types of tumor and some have been shown to be associated with patient prognosis.Materials and methods: In this study, we demonstrated the occurrence of MDM2 splice variants in OSCC tissues. RNA was extracted from 45 OSCC tissues and 17 normal oral mucosa tissues and reverse transcribed into cDNA. Nested PCR was performed to amplify the MDM2 transcripts and the PCR amplicons were cloned into cloning vector pTZ57R/T and sequenced.Results: We demonstrated that 37/45 …
Microrna Profiles Of Oral Squamous Cell Carcinoma (Oscc) Using Formalin-Fixed, Paraffin Embedded (Ffpe) Tissue, Siow M. Y., Ng L. P. K., Abraham M. T., Zaini Z. M., Cheong S. C., Rosnah Binti Zain
Microrna Profiles Of Oral Squamous Cell Carcinoma (Oscc) Using Formalin-Fixed, Paraffin Embedded (Ffpe) Tissue, Siow M. Y., Ng L. P. K., Abraham M. T., Zaini Z. M., Cheong S. C., Rosnah Binti Zain
Prof. Dr. Rosnah Binti Zain
Introduction: MicroRNAs (miRNAs) have previously found to be highly tissue- or disease-specific biomarkers with clinical applicability. However, the role of miRNAs in OSCC progression has yet to be well-studied. Here, we demonstrated the use of FFPE specimens in microarray profiling and quantitative real-time PCR (qPCR) approaches to identify miRNAs that are differentially expressed in OSCC compared with non-tumour tissues.Materials and methods: Four OSCC and four non-tumour FFPE tissues were obtained from the Malaysian Oral Cancer Database and Tumour System (MOCDTBS), coordinated by OCRCC-UM and profiled using Agilent 8 × 15 k mammalian miRNA microarray. Based on microarray results, TaqMan miRNA …
Immunohistochemical Features Of Extra-Nodal Nk/T Cell Lymphoma, Muhamad H. M., Ibrahim N. I., Rosnah Binti Zain
Immunohistochemical Features Of Extra-Nodal Nk/T Cell Lymphoma, Muhamad H. M., Ibrahim N. I., Rosnah Binti Zain
Prof. Dr. Rosnah Binti Zain
NK/T cell lymphoma is a destructive extranodal lymphoproliferative disorder of a putative natural killer (NK) cell lineage. This rare malignant lymphoma is difficult to diagnose due to the histological resemblance to reactive processes hence was once considered as an inflammatory lesion known as lethal midline granuloma or non-healing granuloma. Providentially, the advance in immunophenotyping has permit characterization of NK/T-cell lymphoma, as a distinct entity from other type of lymphoma. It is most commonly involved the nasal cavity, paranasal sinuses and hard palate and as the case we reported here of a NK/T-cell lymphoma in a 29 year old Indonesian man …
High Throughput Mutational Profiling Of Signaling Molecules In Oral Cancer, Zanaruddin , S. N. S., Cheong , S. C., Rahman , Z. A. A., Abd Ghani , W. M. N. W, Mustafa , W. M. W., Rosnah Binti Zain, Kong , Y. H., Hor , S. Y., Teo , S. H.
High Throughput Mutational Profiling Of Signaling Molecules In Oral Cancer, Zanaruddin , S. N. S., Cheong , S. C., Rahman , Z. A. A., Abd Ghani , W. M. N. W, Mustafa , W. M. W., Rosnah Binti Zain, Kong , Y. H., Hor , S. Y., Teo , S. H.
Prof. Dr. Rosnah Binti Zain
It is well established that a plethora of mutations that occur in oncogenes and tumor-suppressor genes are the main drivers of tumorigenesis. The identification of these mutations provides important clues to the cellular processes of carcinogenesis and affords an opportunity for these genes to be used for diagnostic and therapeutic purposes. In comparison to cancers such as breast, lung and colorectal, there is a lack of systematic, large scale characterization of oncogenic mutations oralsquamous cell carcinoma (OSCC).
Genome Wide Profiling Of Tongue And Cheek Cancer Using High Resolution Array Based Cgh, Chong V. K. V., Rahman Z. A. A., Zakaria Z., Tay K. K., Ismail S. M., Rosnah Binti Zain
Genome Wide Profiling Of Tongue And Cheek Cancer Using High Resolution Array Based Cgh, Chong V. K. V., Rahman Z. A. A., Zakaria Z., Tay K. K., Ismail S. M., Rosnah Binti Zain
Prof. Dr. Rosnah Binti Zain
Introduction: Tongue and cheek cancer have different behaviors. In order to understand these behaviors, there is a need to look into the chromosomal alterations and gene pathways that maybe associated with oral cancer at these sites. Therefore, the objective of this study is to determine the differences in chromosomal aberrations and gene pathways involved in tongue and cheek cancer using high resolution array CGH. Methods: A genome wide screening with array CGH (SurePrint G3 CGH 1x1M microarray) was performed using gDNA from 20 snap frozen fresh tissues consisting of 12 tongue and 8 cheek oral squamous cell carcinomas (samples from …
Expression Of Gna12 And Its Role In Oral Cancer, Gan C. P., Rosnah Binti Zain, Abraham M. T., Patel V., Gutkind J. S., Cheong S. C., Chong C. E., Hamid S., Teo S. H.
Expression Of Gna12 And Its Role In Oral Cancer, Gan C. P., Rosnah Binti Zain, Abraham M. T., Patel V., Gutkind J. S., Cheong S. C., Chong C. E., Hamid S., Teo S. H.
Prof. Dr. Rosnah Binti Zain
Introduction: The variability of clinical outcomes in oral cancer patients and the heterogeneity of the disease are the main challenges for the improvement of current treatment modalities. Efforts in our laboratory have focused on the molecular profiling of oral cancer to understand the mechanisms underlying the disease. In a previous microarray study, we found Guanine nucleotide binding protein alpha-12 (GNA12) to be up-regulated in oral cancer. Materials and methods: In this study, we validated the expression of GNA12 at the mRNA level in 47 oral squamous cell carcinoma (OSCC) and 18 non-malignant oral mucosa tissues, by quantitative polymerase chain reaction …
Dietary Consumptions Pattern And Risk Of Oral Cancer: A Malaysian Scenario, Helen-Ng L. C., Ishak I. A., Ghani W. M. N., Marhazlinda J., Norlida A., Rosnah Binti Zain
Dietary Consumptions Pattern And Risk Of Oral Cancer: A Malaysian Scenario, Helen-Ng L. C., Ishak I. A., Ghani W. M. N., Marhazlinda J., Norlida A., Rosnah Binti Zain
Prof. Dr. Rosnah Binti Zain
Introduction: The risk of cancer has been extensively investigated with the intake of single food items but food consumption in an individual is usually complex, made up by combinations of different food items. This study aims to identify dietary patterns of Malaysians, and establishing relationship between dietary pattern and oral cancer risk. Methods A hospital-based, case-control study was conducted on 300 Malaysians who seek treatment at participating hospitals between January 2003 and December 2010. The subjects consisted of 150 cases and 150 controls that were matched for gender, age (5 years) and ethnicity. Dietary intake was measured using Food Frequency …
Effects Of Smoking On Oral Cancer Transcriptome, Rosnah Binti Zain, Karen-Ng L. P., Cheong S. C., Anwar A., Mustaffa W. M. W., Prepagaran N., Zaini Z., Merican A. F., Abraham M. T., Tay K. K., Rahman Z. A. A., Jallaludin A.
Effects Of Smoking On Oral Cancer Transcriptome, Rosnah Binti Zain, Karen-Ng L. P., Cheong S. C., Anwar A., Mustaffa W. M. W., Prepagaran N., Zaini Z., Merican A. F., Abraham M. T., Tay K. K., Rahman Z. A. A., Jallaludin A.
Prof. Dr. Rosnah Binti Zain
Introduction: Oral cancer is a debilitating disease and the survival rates for these patients have not improved over the past decades. Tobacco smoking is one of the most common risk factor associated with oral cancer. Objectives: To explore and identify differential genes expression associated with tobacco smoking. Methodology: Next generation sequencing using the Illumina Genome Analyzer was done to sequence five fresh frozen oral cancer tissue samples from smoking patients, two of which were former smoker and an additional 8 samples consisting of normal oral mucosal tissues from the alveolar mucosa of non-cancer patients where 4 were smokers and another …
Combined Effects Of Isothiocyanates (Itcs) Intake, Glutathione S-Transferases (Gsts) Polymorphism And Risk Habits On Oral Squamous Cell Carcinoma (Oscc) Associated With Earlier Age Of Disease Onset, Karen-Ng L. P., Marhazlinda J., Rahman Z. A. A., Abraham M. T., Cheong S. C., Rosnah Binti Zain
Combined Effects Of Isothiocyanates (Itcs) Intake, Glutathione S-Transferases (Gsts) Polymorphism And Risk Habits On Oral Squamous Cell Carcinoma (Oscc) Associated With Earlier Age Of Disease Onset, Karen-Ng L. P., Marhazlinda J., Rahman Z. A. A., Abraham M. T., Cheong S. C., Rosnah Binti Zain
Prof. Dr. Rosnah Binti Zain
Introduction: ITCs found in cruciferous vegetables has been reported to reduce cancer risk by inducing phase II conjugating enzymes, in particular GSTs. Interestingly, these enzymes also metabolize ITCs therefore; the protective effects of ITCs would depend on the activity of GSTs. This study aimed to determine association between dietary ITCs, GSTs polymorphisms and risk habits (cigarette smoking, alcohol drinking and betel-quid chewing) with oral cancer.Method: Included in this study were 115 OSCC patients and 116 healthy subjects. Information on ITC intake from cruciferous vegetables was collected via a semi-quantitative FFQ. Peripheral blood lymphocytes were obtained for genotyping of GSTM1, GSTT1 …
Association Between Retinol And Vitamin C With The Risk Of Oral Cancer - A Case-Control Study, Vimmitra A., Razak I. A., Helen-Ng L. C., Ghani W. M. N., Abdullah N., Rosnah Binti Zain
Association Between Retinol And Vitamin C With The Risk Of Oral Cancer - A Case-Control Study, Vimmitra A., Razak I. A., Helen-Ng L. C., Ghani W. M. N., Abdullah N., Rosnah Binti Zain
Prof. Dr. Rosnah Binti Zain
Introduction: Dietary intake has been hypothesized to be associated with various diseases including oral cancer. According to studies worldwide, imbalanced diet and nutrient deficiency have been attributed to promote oral malignancies. However, the function of specific nutrients in preventing oral cancer remains largely undefined. This study aims to investigate the association between intake of retinol and vitamin C with the risk of oral cancer. Materials and methods: Dietary intake data was collected from 8 participating hospitals throughout Malaysia using a validated foodfrequency questionnaire (FFQ). Intake of retinol and vitamin C were computed using the Nutriemart software. This case-control study included …
A Simple Technique For Chromosome Preparation From Embryonic Tissues Of Teleosts For Ploidy Verification, Pradeep P. J., Srijaya T. C., Rosnah Binti Zain, Papini A., Chatterji A. K.
A Simple Technique For Chromosome Preparation From Embryonic Tissues Of Teleosts For Ploidy Verification, Pradeep P. J., Srijaya T. C., Rosnah Binti Zain, Papini A., Chatterji A. K.
Prof. Dr. Rosnah Binti Zain
The modified preparation technique developed in the present study has provided an excellent chromosome metaphase spread from the whole body of larvae of the red hybrid tilapia Oreochromis mossambicus (Peters, 1852) X Oreochromis niloticus (Linnaeus, 1758). Unlike other chromosome preparation methods, this modified technique showed a reduction in cell loss while suspending cells during the dropping process. This modified technique can therefore be widely applied not only to various tilapias but also to other teleostean fishes for preparing chromosome spreads. The best treatment parameters for preparing good chromosome spreads from red hybrid tilapia were optimized as 0.01% concentration of colchicine …
Valproic Acid: Growth Inhibition Of Head And Neck Cancer By Induction Of Terminal Differentiation And Senescence, Gan C. P., Hamid S., Hor S. Y., Rosnah Binti Zain, Ismail S. M., Mustafa W. M. W., Teo S. H., Saunders N., Cheong S. C.
Valproic Acid: Growth Inhibition Of Head And Neck Cancer By Induction Of Terminal Differentiation And Senescence, Gan C. P., Hamid S., Hor S. Y., Rosnah Binti Zain, Ismail S. M., Mustafa W. M. W., Teo S. H., Saunders N., Cheong S. C.
Prof. Dr. Rosnah Binti Zain
Background: There are limited studies on the effects of drugs that modulate epigenetic regulation for head and neck squamous cell carcinoma (HNSCC). This study determined the effect of valproic acid (VPA) on HNSCC. Methods: Growth inhibition effects of VPA alone or in combination with 5-aza-2'deoxycytidine (5-aza-dC) or all-trans retinoic acid (ATRA) was evaluated with MTT and clonogenic assays on 5 HNSCC cell lines. The mechanism of growth inhibition was investigated by looking at markers of terminal differentiation and senescence. Results: Growth inhibition profiles of HNSCC cell lines varied in response to VPA. Inhibition of clonogenic survival in response to VPA …
Intercountry Prevalences And Practices Of Betel-Quid Use In South, Southeast And Eastern Asia Regions And Associated Oral Preneoplastic Disorders: An International Collaborative Study By Asian Betel-Quid Consortium Of South And East Asia, Lee C. H., Ko A. M. S., Warnakulasuriya S., Yin B. L., Rosnah Binti Zain, Ibrahim S. O., Liu Z. W., Li W. H., Zhang S. S., Utomo B., Rajapakse P. S., Warusavithana S. A., Abdul Razak I., Abdullah N., Shrestha P., Kwan A. L., Shieh T. Y., Chen M. K., Ko Y. C.
Intercountry Prevalences And Practices Of Betel-Quid Use In South, Southeast And Eastern Asia Regions And Associated Oral Preneoplastic Disorders: An International Collaborative Study By Asian Betel-Quid Consortium Of South And East Asia, Lee C. H., Ko A. M. S., Warnakulasuriya S., Yin B. L., Rosnah Binti Zain, Ibrahim S. O., Liu Z. W., Li W. H., Zhang S. S., Utomo B., Rajapakse P. S., Warusavithana S. A., Abdul Razak I., Abdullah N., Shrestha P., Kwan A. L., Shieh T. Y., Chen M. K., Ko Y. C.
Prof. Dr. Rosnah Binti Zain
Health risks stemming from betel-quid (BQ) chewing are frequently overlooked by people. Updated epidemiological data on the increased BQ use among Asian populations using comparable data collection methods have not been widely available. To investigate the prevalence, patterns of practice and associated types of oral preneoplastic disorders, an intercountry Asian Betel-quid Consortium study (the ABC study) was conducted for Taiwan, Mainland China, Malaysia, Indonesia, Nepal and Sri Lanka. A random sample of 8,922 subjects was recruited, and the data were analyzed using survey-data modules adjusted for the complex survey design. Chewing rates among men (10.7-43.6%) were significantly higher than women …
Human Platelet Lysate Permits Scale-Up Of Dental Pulp Stromal Cells For Clinical Applications, Govindasamy V., Ronald V. S., Abdullah A. N. B., Ganesan Nathan K. R., Aziz Z. A. C. A., Abdullah M., Rosnah Binti Zain, Kasim N. H. A., Musa S., Bhonde R. R.
Human Platelet Lysate Permits Scale-Up Of Dental Pulp Stromal Cells For Clinical Applications, Govindasamy V., Ronald V. S., Abdullah A. N. B., Ganesan Nathan K. R., Aziz Z. A. C. A., Abdullah M., Rosnah Binti Zain, Kasim N. H. A., Musa S., Bhonde R. R.
Prof. Dr. Rosnah Binti Zain
Background aims. Dental pulp stromal cells (DPSC) are considered to be a promising source of stem cells in the field of regenerative therapy. However, the usage of DPSC in transplantation requires large-scale expansion to cater for the need for clinical quantity without compromising current good manufacturing practice (cGMP). Existing protocols for cell culturing make use of fetal bovine serum (FBS) as a nutritional supplement. Unfortunately, FBS is an undesirable additive to cells because it carries the risk of transmitting viral and prion diseases. Therefore, the present study was undertaken to examine the efficacy of human platelet lysate (HPL) as a …
Advancing Oral Medicine Through Informatics And Information Technology: A Proposed Framework And Strategy, Rosnah Binti Zain
Advancing Oral Medicine Through Informatics And Information Technology: A Proposed Framework And Strategy, Rosnah Binti Zain
Prof. Dr. Rosnah Binti Zain
The implementation of information technology in healthcare is a significant focus for many nations around the world. However, information technology support for clinical care, research and education in oral medicine is currently poorly developed. This situation hampers our ability to transform oral medicine into a 'learning healthcare discipline' in which the divide between clinical practice and research is diminished and, ultimately, eliminated. This paper reviews the needs of and requirements for information technology support of oral medicine and proposes an agenda designed to meet those needs. For oral medicine, this agenda includes analyzing and reviewing current clinical and documentation practices, …
Genetic Polymorphisms And Risk Of Oral Cancer, Zaini Z. M., Karen-Ng L. P., Cheong S. C., Gurdeep S. M., Yuen K. M., Tay K. K., Jalil N., Ismail S. M., Jallaluddin A., Rosnah Binti Zain
Genetic Polymorphisms And Risk Of Oral Cancer, Zaini Z. M., Karen-Ng L. P., Cheong S. C., Gurdeep S. M., Yuen K. M., Tay K. K., Jalil N., Ismail S. M., Jallaluddin A., Rosnah Binti Zain
Prof. Dr. Rosnah Binti Zain
Objectives: This study was done to investigate the role of single nucleotide polymorphisms(SNPs) within genes of phase I (CYP1A1) and phase II (GSTM1, GSTT1,GSTP1) of the xenobiotic metabolism and its association with oral cancer risk.Methods: An unmatched case-control study was conducted using 207 newly diagnosed oral cancer patients and 117 non-cancer subjects selected from the OCRCC database. Peripheral blood was obtained from consented individuals and the CYP1A1, GSTM1, GSTT1 and GSTP1 genotypes were determined using polymerase chain reaction (PCR) and restriction enzyme digestion (RFLP). Simple and multiple logistic regression yielding odds ratio (OR and aOR) were employed to measure the …
Transcriptional Profiling Of Oral Squamous Cell Carcinoma Using Formalin-Fixed Paraffin-Embedded Samples, Saleh A., Rosnah Binti Zain, Hussaini H., Ng F., Tanavde V., Hamid S., Chow A. T., Lim G. S., Abraham M. T., Teo S. H., Cheong S. C.
Transcriptional Profiling Of Oral Squamous Cell Carcinoma Using Formalin-Fixed Paraffin-Embedded Samples, Saleh A., Rosnah Binti Zain, Hussaini H., Ng F., Tanavde V., Hamid S., Chow A. T., Lim G. S., Abraham M. T., Teo S. H., Cheong S. C.
Prof. Dr. Rosnah Binti Zain
Despite the advances in cancer treatment, the 5-year survival rate for oral cancer has not changed significantly for the past 40 years and still remains among the worst of all anatomic sites. Gene expression microarrays have been used successfully in the identification of genetic alterations in cancer development, however, these have hitherto been limited by the need for specimens with good quality intact RNA. Here, we demonstrated the use of formalin-fixed paraffin-embedded tissues in microarray experiments to identify genes differentially expressed between cancerous and normal oral tissues. Forty-three tissue samples were macrodissected and gene expression analyses were conducted using the …
A Bootstrap-Anfis Framework For Oral Cancer Prognosis Based On Clinical And Genomic Markers, Chang S. W., Sameem A. K., Rosnah Binti Zain, Merican A. F. M. A.
A Bootstrap-Anfis Framework For Oral Cancer Prognosis Based On Clinical And Genomic Markers, Chang S. W., Sameem A. K., Rosnah Binti Zain, Merican A. F. M. A.
Prof. Dr. Rosnah Binti Zain
The incidence of oral cancer is high in Malaysia especially for those of Indian ethnic origin. In this research, an oral cancer prognosis prediction model will be developed by using Bootstrap-ANFIS techniques. The aim of this research is to improve the prediction of oral cancer survival and to identify the key clinical and genomic markers for the survival prediction of oral cancer. The proposed model is designed to handle small medical dataset with limitations in terms of time, cost and tissue samples.
Gene Expression In Human Oral Squamous Cell Carcinoma Is Influenced By Risk Factor Exposure, Cheong S. C., Chandramouli G. V. R., Saleh A., Rosnah Binti Zain, Lau S. H., Sivakumaren S., Pathmanathan R., Prime S. S., Teo S. H., Patel V., Gutkind J. S.
Gene Expression In Human Oral Squamous Cell Carcinoma Is Influenced By Risk Factor Exposure, Cheong S. C., Chandramouli G. V. R., Saleh A., Rosnah Binti Zain, Lau S. H., Sivakumaren S., Pathmanathan R., Prime S. S., Teo S. H., Patel V., Gutkind J. S.
Prof. Dr. Rosnah Binti Zain
Oral squamous cell carcinoma (OSCC) is a world health problem and is associated with exposure to different risk factors. In the west, smoking and alcohol consumption are considered to be the main risk factors whilst in India and southeast Asia, betel quid (BQ) chewing is predominant. In this study, we compared the gene expression patterns of oral cancers associated with BQ chewing to those caused by smoking using Affymetrix microarrays. We found that 281 genes were differentially expressed between OSCC and normal oral mucosa regardless of aetiological factors including MMP1, PLAU, MAGE-D4, GNA12, IFITM3 and NMU. Further, we identified 168 …
Cyp1a1, Gstm1 And Gstt1 Polymorphisms And Oral Cancer - Correlation With Patients' Status At 2-Year, Age Of Onset, Nodal Status, Tumor Size And Stage, Abraham M. T., Karen-Ng L. P., Ghani W. M. N., Rosnah Binti Zain, Raja-Latifah R. J., Cheong S. C.
Cyp1a1, Gstm1 And Gstt1 Polymorphisms And Oral Cancer - Correlation With Patients' Status At 2-Year, Age Of Onset, Nodal Status, Tumor Size And Stage, Abraham M. T., Karen-Ng L. P., Ghani W. M. N., Rosnah Binti Zain, Raja-Latifah R. J., Cheong S. C.
Prof. Dr. Rosnah Binti Zain
Altered expression of xenobiotic enzymes such as CYP1A1, GSTM1 and GSTT1 has been reported in some malignant tumors including oral cancer. However, the correlation between these enzymes and clinicopathologic parameters has not been well documented. The aim of this study was to investigate the associations between CYP1A1, GSTM1 and GSTT1 polymorphisms with patients’ status at 2-year, age of onset, nodal status, tumor size and disease stage. A total of 195 oral cancer patients were included in this study. Peripheral blood was obtained from consented individuals and CYP1A1, GSTM1 and GSTT1 genotypes were determined using PCR and restriction enzyme digestion. Chi-square …
Metastatic Follicular Thyroid Carcinoma To The Mandible: A Case Report, Ismail , S. B., Abraham T. , M., Zaini , Z. B., Yaacob , H. B., Rosnah Binti Zain
Metastatic Follicular Thyroid Carcinoma To The Mandible: A Case Report, Ismail , S. B., Abraham T. , M., Zaini , Z. B., Yaacob , H. B., Rosnah Binti Zain
Prof. Dr. Rosnah Binti Zain
Introduction: Metastatic lesions to the oro-facial region may be the first evidence of dissemination of an unknown tumour from its primary site. Case presentation: We described a case of metastatic follicular thyroid carcinoma to the mandible presenting with pain and loosening of teeth in a 70 years old female patient leading to extraction of the loose teeth. Conclusion: The present case emphasizes the importance of considering metastasis in the differential diagnosis of swelling related to loosening of teeth, even though the patient had no history of any malignant disease.
Mdm2 Snp309 Does Not Confer An Increased Risk To Oral Squamous Cell Carcinoma But May Modulate The Age Of Disease Onset, Hamid S., Yang Y. H., Peng K. N. L., Ismail S. M., Rosnah Binti Zain, Lim K. P., Wan Mustafa W. M., Abraham M. T., Teo S. H., Cheong S. C.
Mdm2 Snp309 Does Not Confer An Increased Risk To Oral Squamous Cell Carcinoma But May Modulate The Age Of Disease Onset, Hamid S., Yang Y. H., Peng K. N. L., Ismail S. M., Rosnah Binti Zain, Lim K. P., Wan Mustafa W. M., Abraham M. T., Teo S. H., Cheong S. C.
Prof. Dr. Rosnah Binti Zain
The MDM2 SNP309 has been associated with increased expression of the protein which could suppress p53 function, and has been shown to modulate risk to cancer. We have previously shown that overexpression of MDM2 is a common event in oral cancers. In the present study, we determined the association between the MDM2 SNP309 polymorphism and oral cancer in 207 oral cancer patients and 116 normal subjects. We genotyped the MDM2 SNP309 by PCR-RFLP. Logistic regression was adapted to calculate odds ratios for MDM2 SNP309 polymorphism from univariate and multivariable adjusted models. Our results suggest that MDM2 SNP309 does not confer …
Intracanal Bisphosphonate Does Not Inhibit Replacement Resorption Associated With Delayed Replantation Of Monkey Incisors, Thong Y. L., Messer H. H., Rosnah Binti Zain, Saw L. H., Yoong L. T.
Intracanal Bisphosphonate Does Not Inhibit Replacement Resorption Associated With Delayed Replantation Of Monkey Incisors, Thong Y. L., Messer H. H., Rosnah Binti Zain, Saw L. H., Yoong L. T.
Prof. Dr. Rosnah Binti Zain
Progressive replacement resorption following delayed replantation of avulsed teeth has proved to be an intractable clinical problem. A wide variety of therapeutic approaches have failed to result in the predictable arrest of resorption, with a good long-term prognosis for tooth survival. Bisphosphonates are used in the medical management of a range of bone disorders and topically applied bisphosphonate has been reported to inhibit root resorption in dogs. This study evaluated the effectiveness of a bisphosphonate (etidronate disodium) as an intracanal medicament in the root canals of avulsed monkey teeth, placed before replantation after 1 h of extraoral dry storage. Incisors …
Gstm1, Gstt1 And Cyp1a1 Polymorphisms And Risk Of Oral Cancer: A Case-Control Study In Jakarta, Indonesia, Amtha R., Ching C. S., Rosnah Binti Zain, Razak I. A., Basuki B., Roeslan B. O., Gautama W., Purwanto D.
Gstm1, Gstt1 And Cyp1a1 Polymorphisms And Risk Of Oral Cancer: A Case-Control Study In Jakarta, Indonesia, Amtha R., Ching C. S., Rosnah Binti Zain, Razak I. A., Basuki B., Roeslan B. O., Gautama W., Purwanto D.
Prof. Dr. Rosnah Binti Zain
Purpose: to investigate genetic polymorphisms in GSTM1, GSTT1 and CYP1A1 and the association with the risk of oral cancer in the Jakarta population. Method: A total of 81 cases and 162 controls matched for age and sex were selected from 5 hospitals in Jakarta. Sociodemographic data using questionnaires were obtained and peripheral blood samples were collected with informed consent for PCR-RFLP assay. Conditional logistic regression analysis was performed to obtain the association between the risk of oral cancer and GSTM1, GSTT1 and CYP1A1 polymorphisms. Results: GSTM1 and GSTT1 null were slightly overrepresented among cases (60.5% and 45.7% respectively) compared to …
Establishment And Characterization Of Asian Oral Cancer Cell Lines As In Vitro Models To Study A Disease Prevalent In Asia, Hamid S., Lim K. P., Rosnah Binti Zain, Ismail S. M., Lau S. H., Mustafa W. M. W., Abraham M. T., Nam N. A., Teo S. H., Cheong S. C.
Establishment And Characterization Of Asian Oral Cancer Cell Lines As In Vitro Models To Study A Disease Prevalent In Asia, Hamid S., Lim K. P., Rosnah Binti Zain, Ismail S. M., Lau S. H., Mustafa W. M. W., Abraham M. T., Nam N. A., Teo S. H., Cheong S. C.
Prof. Dr. Rosnah Binti Zain
We have established 3 cell lines ORL-48, -115 and -136 from surgically resected specimens obtained from untreated primary human oral squamous cell carcinomas of the oral cavity. The in vitro growth characteristics, epithelial origin, in vitro anchorage independency, human papillomavirus (HPV) infection, microsatellite instability status, karyotype and the status of various cell cycle regulators and gatekeepers of these cell lines were investigated. All 3 cell lines grew as monolayers with doubling times ranging between 26.4 and 40.8 h and were immortal. Karyotyping confirmed that these cell lines were of human origin with multiple random losses and gains of entire chromosomes …
Oral Lichen Planus And Lichenoid Reactions: Etiopathogenesis, Diagnosis, Management And Malignant Transformation, Ismail S. B., Kumar S. K., Rosnah Binti Zain
Oral Lichen Planus And Lichenoid Reactions: Etiopathogenesis, Diagnosis, Management And Malignant Transformation, Ismail S. B., Kumar S. K., Rosnah Binti Zain
Prof. Dr. Rosnah Binti Zain
Lichen planus, a chronic autoimmune, mucocutaneous disease affects the oral mucosa (oral lichen planus or OLP) besides the skin, genital mucosa, scalp and nails. An immune mediated pathogenesis is recognized in lichen planus although the exact etiology is unknown. The disease most commonly affects middle-aged females. Oral lichenoid reactions (OLR) which are considered variants of OLP, may be regarded as a disease by itself or as an exacerbation of an existing OLP, by the presence of medication (lichenoid drug reactions) or dental materials (contact hypersensitivity). OLP usually presents as white striations (Wickham's striae), white papules, white plaque, erythema, erosions or …
An Adaptive Fuzzy Regression Model For The Prediction Of Dichotomous Response Variables, Dom R. M., Kareem S. A., Rosnah Binti Zain, Abldin B.
An Adaptive Fuzzy Regression Model For The Prediction Of Dichotomous Response Variables, Dom R. M., Kareem S. A., Rosnah Binti Zain, Abldin B.
Prof. Dr. Rosnah Binti Zain
This paper proposes an adaptive technique in the prediction of dichotomous response variable by combining fuzzy concept with statistical logistic regression. The model was tested on an oral cancer dataset in predicting oral cancer susceptibility. In this paper we will present the development, evaluation and validation of the proposed model based on the experiment carried out. Explanatory power of the adaptive model was calculated and compared with fuzzy neural network and statistical logistic regression models using calibration and discrimination techniques. Area under ROC values calculated indicates that the proposed model has compatible predictive ability to both fuzzy neural network and …
Histopathologic Findings And Assessment Of Cervical Lymph Node Metastasis In Oral Squamous Cell Carcinoma Of Malaysian Patients, Ismail S. B., Rosnah Binti Zain, Kipli N. P., Abraham T., Ismail M. S., Rahman Z. A. A., Prepageran N., Jalaludin M. A. B.
Histopathologic Findings And Assessment Of Cervical Lymph Node Metastasis In Oral Squamous Cell Carcinoma Of Malaysian Patients, Ismail S. B., Rosnah Binti Zain, Kipli N. P., Abraham T., Ismail M. S., Rahman Z. A. A., Prepageran N., Jalaludin M. A. B.
Prof. Dr. Rosnah Binti Zain
Purpose: To evaluate some histopathologic parameters and relate with the occurrence of cervical lymph node metastasis in oral squamous cell carcinoma (OSCC) in Malaysian patients. Methods: A total of 44 cases of OSCC resected were included. The resected specimens were examined for the OSCC type, degree of differentiation, pattern of invasion (at the tumour invasive front), greatest dimension and the depth of stromal invasion. Where there is no neck resection, the clinical nodal status was obtained from the Oral Cancer database maintained at the University of Malaya Oral Cancer Research and Coordinating Centre (UM-OCRCC) while the histopathologic data were obtained …
Immunoexpression Of Prb And Egfr Using Tissue Microarray (Tma) Technique - A Preliminary Study, Kipli N. P., Cheong C. S., Rosnah Binti Zain, Hamid S., Lim K. P., Abraham T., Ismail M. S., Hussaini H. M.
Immunoexpression Of Prb And Egfr Using Tissue Microarray (Tma) Technique - A Preliminary Study, Kipli N. P., Cheong C. S., Rosnah Binti Zain, Hamid S., Lim K. P., Abraham T., Ismail M. S., Hussaini H. M.
Prof. Dr. Rosnah Binti Zain
Purpose: To assess the expression of pRb and EGFR in oral epithelial lesions using TMA technique. Methods: Paraffin embedded specimens were selected from the archives of the Diagnostic Laboratories at the Faculty of Dentistry, University of Malaya and Universiti Kebangsaan Malaysia. Selected epithelial areas were cored and developed into tissue microarray blocks. A total of 32 samples for pRb and 50 samples for EGFR were analysed using immunohistochemical techniques. Results: Abnormal pRb staining (1+ or less) was seen in 80% (8/10) OSCC, 38.5% (5/13) dysplastic and 22.2% (2/9) normal/hyperplastic epithelium. Positive EGFR staining was observed in 25/35 (71.4%) OSCC cases …